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Inhibitory Effects of the Methylene Chloride Fraction of JP05 on the Production of Inflammatory Mediators in LPS-activated BV2 Microglia

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Abstract

Excessive production of inflammatory mediators such as nitric oxide (NO) and proinflammatory cytokines from activated microglia in the central nervous system contributes to uncontrolled inflammation in neurodegenerative disorders. In this study, we investigated the anti-inflammatory activities of the methylene chloride fraction of JP05 (JP05-MC) on the production of inflammatory mediators in lipopolysaccharide (LPS)-stimulated BV2 mouse microglial cells, and its mechanism of action. JP05-MC significantly inhibited LPS-induced production of NO and the proinflammatory cytokines, TNF-α and IL-6, in BV2 cells. JP05-MC also attenuated the mRNA expression and protein levels of inducible nitric oxide synthase in LPS-induced BV2 cells. JP05-MC significantly attenuated LPS-elicited phosphorylation of the mitogen-activated protein kinase (MAPK), extracellular-signal-regulated kinase 1/2, and nuclear factor-κB (NF-κB) nuclear translocation in BV2 cells. Our results indicate that JP05-MC exerts anti-inflammatory properties via downregulation of inflammatory mediator gene transcription by suppressing the MAPK and NF-κB pathways, suggesting that JP05-MC may have therapeutic potential as an anti-inflammatory agent in neurodegenerative diseases.

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Acknowledgments

This study was supported by a grant from the Oriental Medicine R&D Project, Ministry of Health, Welfare and Family Affairs (B080027) and by the National Research Foundation Grant funded by the Korean Government (KRF-2009-353-E00035), Republic of Korea.

Conflict of Interest

The authors have declared that there is no conflict of interest.

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Authors and Affiliations

  1. Oriental Medicine R&D Center, College of Oriental Medicine, Dongguk University, Gyeongju, 780-714, Republic of Korea

    Hyo Won Jung, Je-Hyun Lee & Yong-Ki Park

  2. Department of Herbology, College of Oriental Medicine, Dongguk University, Gyeongju, 780-714, Republic of Korea

    Tae Woo Oh, Jin Ki Jung, Je-Hyun Lee & Yong-Ki Park

  3. Department of Oriental Internal Medicine, Dongguk University Bundang Oriental Hospital, Gyeoggido, 463-865, Republic of Korea

    Gil Jo Shin

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  1. Hyo Won Jung
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Correspondence to Yong-Ki Park.

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Jung, H.W., Oh, T.W., Jung, J.K. et al. Inhibitory Effects of the Methylene Chloride Fraction of JP05 on the Production of Inflammatory Mediators in LPS-activated BV2 Microglia. Inflammation 35, 332–341 (2012). https://doi.org/10.1007/s10753-011-9323-7

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