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Inflammation

, Volume 35, Issue 1, pp 183–191 | Cite as

Effect of Scrophularia buergeriana Extract on the Degranulation of Mast Cells and Ear Swelling Induced by Dinitrofluorobenzene in Mice

  • Jin-Kyung Kim
  • Yoon Hee Kim
  • Hee Hwan Lee
  • Soon Sung Lim
  • Kyung Woo Park
Article

Abstract

Scrophularia buergeriana Miquel (Scrophulariaceae, SB) is a biennial plant native to Korea, northern China, and Japan that plays an important role in traditional medicine. The dried root of SB has long been used in oriental medicine for treatment of fever, swelling, constipation, pharyngitis, neuritis, and laryngitis. In the present study, we evaluated the ethanol extract of SB (SBE) to determine if it exerted any anti-allergic effects that had not previously been demonstrated. SBE markedly inhibited β-hexosaminidase and histamine release and suppressed the expression of tumor necrosis factor-α and interleukin-4 cytokines by RBL-2H3 mast cells. In addition, topical treatment with SBE effectively reduced allergic inflammation in a dinitrofluorobenzene-induced contact hypersensitivity mouse model. These results strongly suggest that SBE is a promising source of anti-allergic agents.

KEY WORDS

Scrophularia buergeriana allergy inflammation RBL-2H3 cell 

Notes

ACKNOWLEDGMENTS

This research was supported by research grants from Catholic University of Daegu in 2010.

Supplementary material

10753_2011_9304_MOESM1_ESM.doc (320 kb)
Supplementary Fig. 1 (DOC 319 kb)

REFERENCES

  1. 1.
    Duke, J.A., and Ayensu, E.S., 1985. Medicinal Plants of China (2 Vols). 599–601. Reference Publications Inc.: Michigan.Google Scholar
  2. 2.
    Quin, J., D. Hunkler, and H. Rimpler. 1992. Iridoid-related aglycone and its glycosides from Scrophularia ningpoensis. Phytochemistry 31: 905–911.Google Scholar
  3. 3.
    Calis, I., G. Gross, and O. Sticher. 1987. Phenylpropanoid glycosides isolated from Scrophularia scopolii. Phytochemistry 26: 2057–2061.CrossRefGoogle Scholar
  4. 4.
    Kajimoto, T., M. Hidaka, K. Shoyama, and T. Nohara. 1989. Iridoids from Scrophularia ninpoensis. Phytochemistry 28: 2701–2704.CrossRefGoogle Scholar
  5. 5.
    Li, Y.M., S.H. Jiang, W.Y. Gao, and D.Y. Zhu. 2000. Phenylpropanoid glycosides from Scrophularia ningpoensis. Phytochemistry 54: 923–925.PubMedCrossRefGoogle Scholar
  6. 6.
    Kim, S.J., J.S. Park, N.Y. Myung, P.D. Moon, I.Y. Choi, H.J. An, N.H. Kim, H.J. Na, D.H. Kim, M.C. Kim, N.H. An, I.K. Kim, J.Y. Lee, H.J. Jeong, J.Y. Um, H.M. Kim, and S.H. Hong. 2009. Scrophularia buergeriana regulates cytokine production in vitro. Immunopharmacology and Immunotoxicology 31: 246–252.PubMedCrossRefGoogle Scholar
  7. 7.
    Jeong, E.J., K.Y. Lee, S.H. Kim, S.H. Sung, Y. Kim, and C. Kim. 2008. Cognitive-enhancing and antioxidant activities of iridoid glycosides from Scrophularia buergeriana in scopolamine-treated mice. European Journal of Pharmacology 588: 78–84.PubMedCrossRefGoogle Scholar
  8. 8.
    Kim, S.R., K.Y. Lee, K.A. Koo, S.H. Sung, N.G. Lee, J. Kim, and Y.C. Kim. 2002. Four new neuroprotective iridoid glycosides from Scrophularia buergeriana roots. Journal of Natural Products 65: 1696–1699.PubMedCrossRefGoogle Scholar
  9. 9.
    Kim, S.R., S.Y. Kang, K.Y. Lee, S.H. Kim, G.J. Markelonis, T.H. Oh, and Y.C. Kim. 2003. Anti-amnestic activity of E-p-methoxycinnamic acid from Scrophularia buergeriana. Brain Research. Cognitive Brain Research 17: 454–461.PubMedCrossRefGoogle Scholar
  10. 10.
    Lee, E.J., S.R. Kim, J. Kim, and Y.C. Kim. 2002. Hepatoprotective phenylpropanoids from Scrophularia buergeriana roots against CCl4-induced toxicity: action mechanism and structure–activity relationship. Planta Medica 68: 407–411.PubMedCrossRefGoogle Scholar
  11. 11.
    Rajan, T.V. 2003. The Gell–Coombs classification of hypersensitivity reactions: a re-interpretation. Trends in Immunology 24: 376–379.PubMedCrossRefGoogle Scholar
  12. 12.
    Andersson, K., and J. Lidholm. 2003. Characteristics and immunobiology of grass pollen allergens. International Archives of Allergy and Immunology 130: 87–107.PubMedCrossRefGoogle Scholar
  13. 13.
    Gould, H.J., B.J. Sutton, A.J. Beavil, R.L. Beavil, N. McCloskey, H.A. Coker, D. Fear, and L. Smurthwaite. 2003. The biology of IgE and the basis of allergic disease. Annual Review of Immunology 21: 579–628.PubMedCrossRefGoogle Scholar
  14. 14.
    Broide, D.H. 2001. Molecular and cellular mechanisms of allergic disease. The Journal of Allergy and Clinical Immunology 108(2): S65–S71.PubMedCrossRefGoogle Scholar
  15. 15.
    Kabu, K., S. Yamasaki, D. Kamimura, Y. Ito, A. Hasegawa, E. Sato, H. Kitamura, K. Nishida, and T. Hirano. 2006. Zinc is required for Fc epsilon RI-mediated mast cell activation. Journal of Immunology 177: 1296–1305.Google Scholar
  16. 16.
    Gilfillan, A.M., and J. Rivera. 2009. The tyrosine kinase network regulating mast cell activation. Immunological Reviews 228: 149–169.PubMedCrossRefGoogle Scholar
  17. 17.
    Matsuda, H., S. Tewtrakul, T. Morikawa, A. Nakamura, and M. Yoshikawa. 2004. Anti-allergic principles from Thai zedoary: structural requirements of curcuminoids for inhibition of degranulation and effect on the release of TNF-α and IL-4 in RBL-2H3 cells. Bioorganic & Medicinal Chemistry 12: 5891–5898.CrossRefGoogle Scholar
  18. 18.
    Theoharidies, T.C., and D. Kalogeromitros. 2006. The critical role of mast cells in allergy and inflammation. Annals of the New York Academy of Sciences 1088: 78–92.CrossRefGoogle Scholar
  19. 19.
    Asher, M.I., Montefort, S., Björkstén, B., Lai, C.K., Strachan, D.P., and Weiland, S.K., ISAAC Phase Three Study Group. 2006. Worldwide time trends in the prevalence of symptoms of asthma, allergic rhinoconjunctivitis, and eczema in childhood: ISAAC phases one and three repeat multicountry cross-sectional surveys. Lancet 26: 733–743.Google Scholar
  20. 20.
    Holgate, S.T., and R. Polosa. 2008. Treatment strategies for allergy and asthma. Nature Reviews. Immunology 8: 218–230.PubMedCrossRefGoogle Scholar
  21. 21.
    Metzger, H. 1992. The receptor with high affinity for IgE. Immunological Reviews 125: 37–48.PubMedCrossRefGoogle Scholar
  22. 22.
    Olmos, A., R.R. Giner, M.C. Recio, J.L. Rios, J.M. Cerdá-Nicolás, and S. Máñez. 2007. Effects of plant alkylphenols on cytokine production, tyrosine nitration and inflammatory damage in the effect phase of contact hypersensitivity. British Journal of Pharmacology 152: 366–373.PubMedCrossRefGoogle Scholar
  23. 23.
    Kawai, M., T. Hirano, S. Higa, J. Arimitsu, M. Maruta, Y. Kuwahara, T. Ohkawara, K. Hagihara, T. Yamadori, Y. Shima, A. Ogata, I. Kawase, and T. Tanaka. 2007. Flavonoids and related compounds as anti-allergic substances. Allergology International 56: 113–123.PubMedCrossRefGoogle Scholar
  24. 24.
    Rivera, J., and A.M. Gilfillan. 2006. Molecular regulation of mast cell activation. The Journal of Allergy and Clinical Immunology 117: 1214–1225.PubMedCrossRefGoogle Scholar
  25. 25.
    Ross, R., and A.B. Reske-Kunz. 2001. The role of NO in contact hypersensitivity. International Immunopharmacology 1: 1469–1478.PubMedCrossRefGoogle Scholar
  26. 26.
    Grabbe, S., and T. Schwarz. 1998. Immunoregulatory mechanisms involved in elicitation of allergic contact hypersensitivity. Immunology Today 19: 37–44.PubMedCrossRefGoogle Scholar
  27. 27.
    Chen, B., Y. Liu, H.W. Liu, N.L. Wang, B.F. Yang, and X.S. Yao. 2008. Iridoid and aromatic glycosides from Scrophularia ningpoensis Hemsl. and their inhibition of [Ca2+](i) increase induced by KCl. Chemistry & Biodiversity 5: 1723–1735.CrossRefGoogle Scholar
  28. 28.
    Li, J., X. Huang, X. Du, W. Sun, and Y. Zhang. 2009. Study of chemical composition and antimicrobial activity of leaves and roots of Scrophularia ningpoensis. Natural Product Research 23: 775–780.PubMedCrossRefGoogle Scholar
  29. 29.
    Tasdemir, D., N.D. Güner, R. Perozzo, R. Brun, A.A. Dönmez, I. Calis, and P. Rüedi. 2005. Anti-protozoal and plasmodial FabI enzyme inhibiting metabolites of Scrophularia lepidota roots. Phytochemistry 66: 355–362.PubMedCrossRefGoogle Scholar
  30. 30.
    Díaz, A.M., M.J. Abad, L. Fernández, A.M. Silván, J. De Santos, and P. Bermejo. 2004. Phenylpropanoid glycosides from Scrophularia scorodonia: in vitro anti-inflammatory activity. Life Sciences 74: 2515–3226.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Jin-Kyung Kim
    • 1
    • 4
  • Yoon Hee Kim
    • 2
  • Hee Hwan Lee
    • 2
  • Soon Sung Lim
    • 3
  • Kyung Woo Park
    • 1
  1. 1.Department of Biomedical ScienceCatholic University of DaeguGyeongsan-SiSouth Korea
  2. 2.Center for Efficacy Assessment and Development of Functional Foods and DrugsHallym UniversityChuncheonSouth Korea
  3. 3.Department of Food Science and NutritionHallym UniversityChuncheonSouth Korea
  4. 4.Department of Biomedical ScienceGyeongsan-SiSouth Korea

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