Carbon Nanotubes Elicit DNA Damage and Inflammatory Response Relative to Their Size and Shape
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Carbon nanotubes (CNTs) have been one of the most extensively researched and developed nanomaterials. However, little concern has been placed on their safety. The biological effects of CNTs are believed to differ relative to size and shape. Thus, the relationship between the characteristics of CNTs and their safety needs to be evaluated. In this study, we examined the biological effects of different-sized multi-walled CNTs (MWCNTs) and single-walled CNTs (SWCNTs). Long and thick MWCNTs induced the strongest DNA damage while similar SWCNTs caused little effect. Comparison of inflammatory responses of various types of CNTs found that peritoneal CNT administration of long and thick MWCNTs increased the total cell number in abdominal lavage fluid in mice. These results indicate that long and thick MWCNT, but not short and thin MWCNT, cause DNA damage and severe inflammatory effects. These findings might provide useful information for constructing novel CNTs with safety.
KEY WORDScarbon nanotubes cytotoxicity DNA damage inflammation nanomaterial
This study was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and from the Japan Society for the Promotion of Science (JSPS). This study was also supported in part by Health Labor Sciences Research Grants from the Ministry of Health, Labor, and Welfare of Japan; by Health Sciences Research Grants for Research on Health Sciences focusing on Drug Innovation from the Japan Health Sciences Foundation; by a Grant from the Minister of the Environment; and by The Nagai Foundation Tokyo.
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