Therapeutic Effect of Recombinant Human Catalase on H1N1 Influenza-induced Pneumonia in Mice
- 209 Downloads
Reactive oxygen species (ROS) are believed to play a key role in the induction of lung damage caused by pneumonia and therapeutic agents that could effectively scavenge ROS may prevent or reduce the deleterious effects of influenza-induced pneumonia. In this study, we first demonstrated that human catalase could attenuate acute oxidative injury in lung tissues following influenza-induced pneumonia. Mice were infected with influenza virus H1N1 (FM1 strain) and treated with recombinant human catalase (50,000 U/kg) by inhalation. The survival time and survival rates of H1N1 induced pneumonia mice were increased by treatment with recombinant human catalase. Protective efficacy of catalase was also observed in lung histology, anti-oxidant parameters, pulmonary pathology and influenza viral titer in lungs in mice. These observations were associated with increased serum superoxide and hydroxyl radical anion scavenging capacities. This study strongly indicated that recombinant catalase might be a potential therapy for H1N1 influenza-induced pneumonia.
KEY WORDShuman catalase influenza pneumonia oxidative injury
This work was supported by grants from the National Nature Science Foundation of China (No: 30572276, 2006–2008), Shanghai Science and Technology Funds (No: 09XD1421800, 06DZ22906) and National Science and Technology Major Project for Drug Discovery of Ministry of Science and Technology of China (2009ZX09303).
- 13.Hyoudou, K., M. Nishikawa, Y. Umeyama, Y. Kobayashi, F. Yamashita, and M. Hashida. 2004. Inhibition of metastatic tumor growth in mouse lung by repeated administration of polyethylene glycol-conjugated catalase: Quantitative analysis with firefly luciferase-expressing melanoma cells. Clin Cancer Res 10: 7685–7691.CrossRefPubMedGoogle Scholar
- 18.Tao, P., M.C. Luo, R.G. Pan, D.W. Ling, S.Y. Zhou, P. Tien, and Z.S. Pan. 2009. Enhanced protective immunity against H5N1 influenza virus challenge by vaccination with DNA expressing a chimeric hemagglutinin in combination with an MHC class I-restricted epitope of nucleoprotein in mice. Antiv Res 81: 253–260.CrossRefGoogle Scholar
- 20.World Health Organization Weekly Epidemiological Record. 2009. Global influenza surveillance network: laboratory surveillance and response to pandemic H1N1 2009. 84: 361–372.Google Scholar
- 21.Fraser, C., C.A. Donnelly, S. Cauchemez, W.P. Hanage, M.D. Van Kerkhove, T.D. Hollingsworth, J. Griffin, R.F. Baggaley, H.E. Jenkins, E.J. Lyons, T. Jombart, W.R. Hinsley, N.C. Grassly, F. Balloux, A.C. Ghani, N.M. Ferguson, A. Rambaut, O.G. Pybus, H. Lopez-Gatell, C.M. Alpuche-Aranda, I.B. Chapela, E.P. Zavala, D.M. Espejo Guevara, F. Checchi, E. Garcia, S. Hugonnet, and C. Roth. 2009. Pandemic potential of a strain of Influenza A (H1N1): Early findings. Science 324: 1557–1561.CrossRefPubMedGoogle Scholar
- 26.Kim, D.W., H.J. Jeong, H.W. Kang, M.J. Shin, E.J. Sohn, M.J. Kim, E.H. Ahn, J.J. An, S.H. Jang, K.Y. Yoo, M.H. Won, T.C. Kang, I.K. Hwang, O.S. Kwon, S.W. Cho, J. Park, W.S. Eum, and S.Y. Choi. 2009. Transduced human PEP-1-catalase fusion protein attenuates ischemic neuronal damage. Free Radic Biol Med 47: 941–952.CrossRefPubMedGoogle Scholar