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Ozone Therapy in Induced Endotoxemic Shock. II. The Effect of Ozone Therapy Upon Selected Histochemical Reactions in Organs of Rats in Endotoxemic Shock

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Abstract

Mitochondria and lysosomes were evaluated by assessment of changes in activity of selected enzymes: lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), adenosinetriphosphatase (ATPase), acid phosphatase (AcPase) and β-glucuronidase (BG) in rats under profound hypoxia induced by endotoxemic shock. The study was conducted on adult male Wistar rats. The animals formed the following four groups of 15 rats each: control animals (C);—rats receiving intraperitonally O2/O3 (CO), rats receiving of Escherichia coli toxin (LPS) (CL); rats receiving LPS plus oxygen–ozone mixture (OL). Histoenzymatic examinations of liver, kidney, lungs, and heart muscle were performed. Lipopolysaccharide suppressed activities of all the enzymes except for LDH, the activity of which as high as a fourfold increase. The results demonstrated potent, stabilizing and regenerative effects of ozone therapy on body enzymatic processes in course of induced endotoxemic shock in rats, which might prove to be of clinical significance.

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Authors and Affiliations

  1. Department and Clinic of Gynaecological Endocrynology, Medical University of Silesia, ul. Medyków 14, 40-752, Katowice, Poland

    Paweł Madej & Wojciech Mroczka

  2. Department of Protein Chemistry and Enzymology, Medical University of Silesia, ul. Narcyzów 1, 41-200, Sosnowiec, Poland

    Andrzej Plewka

  3. Department of Pathological Anatomy and Forensic Medicine, Veterinary Academy, ul. Norwida 31, 50-375, Wrocław, Poland

    Janusz A. Madej

  4. Department of Histology and Embryology, Medical University of Silesia, ul. Medyków 18, 40-752, Katowice, Poland

    Danuta Plewka

  5. II Department and Clinic of Gynaecology and Obstetrics, Medical University of Silesia, ul. Medyków 14, 40-752, Katowice, Poland

    Krzysztof Wilk

  6. Department of Pathomorphology, Medical University of Silesia, ul. Medyków 18, 40-752, Katowice, Poland

    Zuzanna Dobrosz

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  1. Paweł Madej
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Correspondence to Paweł Madej.

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Madej, P., Plewka, A., Madej, J.A. et al. Ozone Therapy in Induced Endotoxemic Shock. II. The Effect of Ozone Therapy Upon Selected Histochemical Reactions in Organs of Rats in Endotoxemic Shock. Inflammation 30, 69–86 (2007). https://doi.org/10.1007/s10753-007-9023-5

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