, Volume 30, Issue 1–2, pp 14–27 | Cite as

Differential Expression, Time Course and Distribution of Four PARs in Rats with Endotoxin-induced Acute Lung Injury

  • Subrina Jesmin
  • Satoshi Gando
  • Sohel Zaedi
  • Fumika Sakuraya


The hypothesis that the expression of protease-activated receptors (PARs) protein is regulated at the level of transcription and that PAR isoforms, PAR-1, PAR-2, PAR-3, and PAR-4, in lung tissue show different patterns of expression in lipopolysaccharide (LPS)-induced acute lung injury (ALI) was tested. Male Wistar rats were rendered endotoxemic by intra-peritoneal injection of LPS (15 mg/kg body weight). We examined the expression of protein and mRNA and the immunohistochemical localization of PAR isoforms in lung tissues 1, 3, 6, and 10 h after LPS administration. Induction of ALI by LPS was confirmed based on histopathological changes. LPS administration induced significant increases in the expression of PAR isoforms (protein) at the level of transcription in ALI. While the time course of PAR-1 and -2 expressions were different, those of PAR-3 and -4 were almost similar. An immunohistochemical analysis showed localization of PAR isoforms in the vascular endothelium, alveolar epithelium, and alveolar macrophages. However, the cellular distribution patterns of PAR isoforms were different. We conclude that LPS induces increase in protein expression of PAR isoforms at the level of transcription in rats with ALI. The differential expression patterns (over a time course) and distribution of PAR isoforms suggests a distinct role for each isoform in the pathogenesis of LPS-induced ALI.

Key words

acute lung injury (ALI) coagulation endotoxin inflammation protease-activated receptor (PAR) 



This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (2005-17390479). The authors thank Dr. Chishimba N Mowa for his professional proofreading and helpful suggestions.


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Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Subrina Jesmin
    • 1
  • Satoshi Gando
    • 1
  • Sohel Zaedi
    • 1
  • Fumika Sakuraya
    • 1
  1. 1.Division of Acute and Critical Care Medicine, Department of Anesthesiology and Critical Care MedicineHokkaido University Graduate School of MedicineSapporoJapan

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