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Hydrobiologia

, Volume 782, Issue 1, pp 127–143 | Cite as

Environmental factors affecting the balance of autotrophs versus heterotrophs in the microbial food web of temporary ponds

  • Núria Àvila
  • Rocio López-Flores
  • Dani Boix
  • Stéphanie Gascón
  • Xavier D. Quintana
MEDITERRANEAN TEMPORARY PONDS

Abstract

The relative abundance of autotrophic (A) and heterotrophic (H) organisms in the microbial food web of temporary ponds and its relationship to environmental variables were analysed. Four localities (43 temporary ponds) were selected for study. They were located in unaltered, protected basins without intensive agriculture or strong human pressure. Because the supply of allochthonous organic matter is expected to be higher than inorganic nutrient inputs in temporary ponds, we hypothesized that the microbial food web in these unaltered basins was dominated by heterotrophic organisms. Our results showed that the log A:H biomass ratio (A/H) was always negative, indicating strong dominance by heterotrophic organisms in these ponds. Moreover, A/H was negatively related to DOC concentrations, but this relationship was weaker in the locality with the highest DOC concentration. No significant relationships were found between A/H and the other environmental variables analysed, namely inorganic nutrients, fulvic acids, chlorophyll-a concentrations, macrophyte biomass or total zooplankton biomass. However, when the different groups of zooplankton were considered separately, A/H correlated negatively with cladoreran biomass and positively with harpacticoid biomass. Thus, temporary ponds differ from deeper ecosystems, in which the greater importance of autochthonous energy inputs leads to microbial food web that is more dominated by autotrophs.

Keywords

Phytoplankton Bacterioplankton Shallow lakes Temporary waters Dissolved organic carbon Compositional data analysis 

Notes

Acknowledgments

We want to thank the “Departamento de Conservação da Natureza e Florestas do Algarve”, the “Departament d’Agricultura, Ramaderia Pesca, Alimentació i Medi Natural”, the Empordà Wetlands Natural Park and the Authorities of the “Base Militar General Álvarez de Castro” for the permissions to perform our study in the ponds under their management. We also want to thank Simonetta Bagella, Luis Cancela da Fonseca, Maria Carmela Caria, Jordi Compte, Helena Costa, Laura Guirado, Margarida Machado, Albert Ruhí and Lluís Zamora for field assistance. The first author held a PhD grant from the University of Girona (BR2012/02). The financial support was provided by “Ministerio de Ciencia e Innovación” (CGL2011-23907) and the Generalitat de Catalunya (Ref. 2014 SGR 484).

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© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.GRECO, Institute of Aquatic Ecology, Faculty of SciencesUniversity of GironaGironaSpain
  2. 2.Área de Ecología, Departamento de Ciencias Agrarias y del Medio Natural, Escuela Politécnica Superior de Huesca, Instituto de Investigación en Ciencias Ambientales (IUCA)Universidad de ZaragozaHuescaSpain

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