Comparison of genetic diversity in four Typha species (Poales, Typhaceae) from China
- 265 Downloads
Life history traits play an important role in the level and distribution of genetic diversity, and comparing closely related species with similar life histories can provide insight into the determinants of genetic variation in plant populations. In this study, we used variations of one chloroplast DNA fragment, one nuclear gene, and six microsatellites to compare the levels and distributions of genetic diversity in four widespread Typha species from China. Surveys were conducted on 898 individuals from 120 sites. The individuals of all four species formed monophyletic clades and distinct genetic clusters, suggesting no hybridization between T. angustifolia and T. latifolia in China. The levels of cpDNA nucleotide diversity followed the order T. latifolia > T. laxmannii > T. angustifolia > T. orientalis, whereas the genetic diversity in nDNA and nSSR of T. laxmannii and T. angustifolia was higher than that of T. latifolia. In T. angustifolia, T. laxmannii, and T. orientalis, more than half of genetic variation occurred within populations, and in T. latifolia, most of genetic variation occurred among populations. The variation in the levels and distributions of genetic diversity among the four species can be attributed to differences in inflorescence characteristics which either limit or enhanced outcrossing rates.
KeywordsTypha Genetic diversity Chloroplast DNA Nuclear DNA Hybridization China
This study was supported by grants from the National Natural Science Foundation of China to Xinwei Xu (31070190 and 31270265) and Dan Yu (30930011). We thank the members of Dan Yu’s group for field assistance.
Compliance with ethical standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- Alm, C. G. & H. Weimarck, 1933. Typha angustifolia L. × latifolia L. funnen i Skåne. Botaniska Notiser 1933: 279–284.Google Scholar
- Cook, C. D. K., 1990. Aquatic plant book. SPB Academic Publishing, Hague.Google Scholar
- Fér, T., 2008. Study of plant dispersal in river corridors using molecular markers. Ph.D. dissertation, Charles University Prague, Prague.Google Scholar
- Figert, E., 1890. Botanische Mitteilungen aus Schlesien. III. Typha latifolia × Typha angustifolia. Deutsche Botanische Monatsschrift 8: 55–57.Google Scholar
- Goudet, J., 1995. FSTAT: a computer program to calculate statistics, version 1.2. Journal of Heredity 86: 485–486.Google Scholar
- Hamrick, J. L. & M. J. W. Godt, 1989. Allozyme diversity in plant species. In Brown, A. H. D., M. T. Clegg, A. L. Kahler & B. S. Weir (eds), Plant population genetics, breeding and genetic resources. Sinauer Associates, Sunderland: 43–63.Google Scholar
- Hamrick, J. L. & M. J. W. Godt, 1996. Effects of life history traits on genetic diversity in plant species. Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 351: 1291–1298.Google Scholar
- Hartl, D. L., 2000. A primer of population genetics. Sinaue, Sunderland.Google Scholar
- Luther, H. E., 1947. Typha angustifolia × latifolia L. (T. × glauca Godr.) I Ostfennoskandien. Memoranda Societatis Flora et Fauna Fennica 23: 66–75.Google Scholar
- Martins, E., R. Lamont, G. Martinelli, C. Lira-Medeiros, A. Quinet & A. Shapcott, 2014. Genetic diversity and population genetic structure in three threatened Ocotea species (Lauraceae) from Brazil’s Atlantic Rainforest and implications for their conservation. Conservation Genetics 16: 1–14.CrossRefGoogle Scholar
- Ng, W. L., Y. Onishi, N. Inomata, K. M. Teshima, H. T. Chan, S. Baba, S. Changtragoon, I. Z. Siregar & A. E. Szmidt, 2015. Closely related and sympatric but not all the same: genetic variation of Indo-West Pacific Rhizophora mangroves across the Malay Peninsula. Conservation Genetics 16: 137–150.CrossRefGoogle Scholar
- Shaw, J., E. B. Lickey, J. T. Beck, S. B. Farmer, W. Liu, J. Miller, K. C. Siripun, C. T. Winder, E. E. Schilling & R. L. Small, 2005. The tortoise and the hare II: relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis. American Journal of Botany 92: 142–166.CrossRefPubMedGoogle Scholar
- Smith, S. G., 1987. Typha: its taxonomy and the ecological significance of hybrids. Archiv fü Hydrobiologie 27: 129–138.Google Scholar
- Snow, A. A., S. E. Travis, R. Wildová, T. Fér, P. M. Sweeney, J. E. Marburger, S. Windels, B. Kubátová, D. E. Goldberg & E. Mutegi, 2010. Species-specific SSR alleles for studies of hybrid cattails (Typha latifolia × T. angustifolia; Typhaceae) in North America. American Journal of Botany 97: 2061–2067.CrossRefPubMedGoogle Scholar
- Sun, K. & D. Simpson, 2010. Typhaceae. In Wu, Z. Y. & P. H. Raven (eds), Flora of China. vol. 23. Science Press, Beijing: 158–163.Google Scholar