Ecotoxicity of nanosized magnetite to crustacean Daphnia magna and duckweed Lemna minor
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Along with the development of nanotechnology, an increase in production and application of nanosized magnetite (Fe3O4) is expected. Though magnetite is considered relatively safe, information concerning potential hazards of synthetic magnetite nanoparticles with unique physico-chemical characteristics to aquatic organisms is still limited. In this study, we evaluated the toxicity of nanosized (27.2 ± 9.8 nm) and bulk (144.2 ± 67.7 nm) magnetite particles to different life stages of the aquatic crustacean Daphnia magna. In addition, phytotoxicity of the magnetite was evaluated using duckweed Lemna minor. The study did not reveal any statistically significant differences between the biological effects of nanosized and bulk magnetite particles. Both forms of magnetite induced very low toxicity (EC50 > 100 ppm) to D. magna and L. minor in the standard acute assays. However, it was demonstrated that at acutely subtoxic magnetite concentrations (10 and 100 ppm), the number of neonates hatched from D. magna ephippia was decreased. Moreover, short-term (48 h) exposure of neonate daphnids to these concentrations may significantly affect the long-term survival and reproductive potential of daphnids. These results indicate that substantial contamination of aquatic ecosystems by magnetite may disrupt the stability of cladoceran populations.
KeywordsFe3O4 Iron oxide nanoparticles Ecotoxicity Daphnia magna Lemna minor Pollution
This research was supported by the European Regional Development Fund project TERIKVANT, Estonian Targeted Funding project IUT23-5, Estonian Science Foundation project ETF9347. The authors are grateful to Meeri Visnapuu for providing the TEM images of iron oxide particles and to editor A. Petrusek, anonymous reviewers, M. Heinlaan and V. Aruoja for comments and text correction.
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