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Hydrobiologia

, Volume 757, Issue 1, pp 155–165 | Cite as

New insights towards the establishment of phycocyanin concentration thresholds considering species-specific variability of bloom-forming cyanobacteria

  • Inês P. E. Macário
  • Bruno B. Castro
  • Maria I. S. Nunes
  • Sara C. Antunes
  • Cristina Pizarro
  • Carla Coelho
  • Fernando Gonçalves
  • Daniela R. de Figueiredo
Primary Research Paper

Abstract

In vivo phycocyanin (PC) fluorescence allows assessing cyanobacterial abundance in an easy, fast and cost-effective way. However, the establishment of PC thresholds is necessary for its use in routine monitoring programmes and there has been no consensus regarding their definition. This work aimed: (1) to assess the potential species-specific variation in fluorometric PC content among Microcystis aeruginosa, Nostoc muscorum and Cylindrospermopsis raciborskii; (2) to propose specific PC thresholds based on World Health Organization alert levels; and (3) to evaluate the in vivo PC signal reliability to interferences from massive algal growth and mixtures of bloom-forming cyanobacteria. Strong linear relationships were recorded between PC and cell counts, biovolume and chlorophyll a. However, a significant species-specific variation in PC was observed using cell counts. Increased microalgal densities did not cause significant interference of PC signals. Also, dual mixtures of cyanobacteria revealed strong relationships between measured and expected PC content. Results suggest that fluorometric PC is a good predictor for cyanobacterial biomass but cell number is not the best parameter to define thresholds. Biovolume should be used instead. Nevertheless, species-specific thresholds must be considered, rather than a general cyanobacterial threshold.

Keywords

Cyanobacterial blooms Phycocyanin Fluorometry Monitoring Thresholds 

Notes

Acknowledgments

This work was supported by European Funds through COMPETE and by National Funds through the Portuguese Science Foundation (FCT) within project PEst-C/MAR/LA0017/2013. Daniela de Figueiredo and Bruno B. Castro were also supported by FCT, by means of a post-doctoral Grant (SFRH/BPD/74184/2010) and a research contract (programme Ciência2008, co-funded by National Strategic Reference Framework 2007–2013 and European Social Fund), respectively. The authors thank Victor Galhano for providing one of the cyanobacterial cultures.

Supplementary material

10750_2015_2248_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (DOCX 15 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Inês P. E. Macário
    • 1
    • 2
  • Bruno B. Castro
    • 1
    • 2
  • Maria I. S. Nunes
    • 2
    • 3
  • Sara C. Antunes
    • 2
    • 4
  • Cristina Pizarro
    • 5
  • Carla Coelho
    • 5
  • Fernando Gonçalves
    • 1
    • 2
  • Daniela R. de Figueiredo
    • 1
    • 2
  1. 1.Department of BiologyUniversity of AveiroAveiroPortugal
  2. 2.Centre for Environmental and Marine Studies (CESAM)University of AveiroAveiroPortugal
  3. 3.Department of Environment and PlanningUniversity of AveiroAveiroPortugal
  4. 4.Department of Biology, Faculty of SciencesUniversity of PortoPortoPortugal
  5. 5.Water and Soil Unit, Environmental Health DepartmentNational Health Institute Dr. Ricardo Jorge (INSA)PortoPortugal

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