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Hydrobiologia

, Volume 743, Issue 1, pp 109–125 | Cite as

Trophic ecology of dusky grouper Epinephelus marginatus (Actinopterygii, Epinephelidae) in littoral and neritic habitats of southern Brazil as elucidated by stomach contents and stable isotope analyses

  • Mario V. Condini
  • David J. Hoeinghaus
  • Alexandre M. Garcia
Primary Research Paper

Abstract

Groupers are large-bodied fishes with broad global distribution and commercial and ecological importance. Many grouper species are endangered, but information on trophic ecology of groupers outside the Mediterranean Sea is limited. We integrated stomach contents analyses (SCA) and stable isotope analyses (SIA) to test the hypotheses that diets of dusky grouper Epinephelus marginatus differ between littoral and neritic zones in the southwestern Atlantic, but exhibit similar ontogenetic shifts in prey size and consumption of fishes. SCA were dominated by fishes, crabs, and shrimp and differed significantly between habitats, but prey size and consumption of fishes increased with grouper body size at both sites. Grouper stable isotope ratios were similar between sites and among size classes, but integrating SCA and SIA distinguished ingested versus assimilated dietary components, improved dietary resolution, and provided a more comprehensive assessment of grouper in the food webs. Dusky grouper integrate benthic and pelagic trophic pathways, as well as estuarine and marine food webs via consumption of migratory prey. Our findings have applications to other threatened grouper populations, for example, in managing fragmented populations or for reserve designs considering inclusion of artificial substrates as grouper habitat, and more broadly for approaches examining trophic ecology of generalist predators.

Keywords

Food web IUCN red list Man-made habitats Migration Ontogenetic shifts Subsidies 

Notes

Acknowledgments

This study received financial support from the Fundação O Boticário de Proteção a Natureza (0831–20091). MVC and AMG acknowledge fellowship support from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; 140570/2013-6 and 305888/2012-9, respectively). The authors are grateful to L. Madureira for the 3D bathymetric map of Carpinteiro Bank. This is a contribution of the research group “Grupo de Análises de Isótopos Estáveis em Ambientes Aquáticos” (GAIA/CNPq).

Supplementary material

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Supplementary material 4 (DOC 90 kb)

References

  1. Abreu, L. & J. L. Calliari, 2005. Paleocanais na plataforma continental interna do Rio Grande do Sul: evidências de uma drenagem fluvial pretérita. Revista Brasileira de Geofísica 23: 123–132.CrossRefGoogle Scholar
  2. Acha, E. M., 1990. Estudio anatômico-ecologico de La Lisa (Mugil Liza) durante su primer año de vida. Frente Maritimo 7: 37–43.Google Scholar
  3. Alaniz, M., M. F. Heberle & F. D’incao, 2011. Fecundity variation and abundance of female blue crabs Callinectes sapidus Rathbun, 1896 (Decapoda, Brachyura, Portunidae) in the Patos Lagoon estuary, RS, Brazil. Atlântica 33: 141–148.CrossRefGoogle Scholar
  4. Andriguetto, J. & M. Haimovici, 1996. Reproductive cycle of Loligo sanpaulensis Brakoniecki, 1984 (Cephalopoda; Loliginidae) in southern Brazil. Scientia Marina 60: 443–450.Google Scholar
  5. Azevedo, J. M. N., J. B. Rodríguez, M. Mendizábal & L. M. Arruda, 1995. Study of a sample of dusky groupers, Epinephelus marginatus (Lowe, 1834), caught in the tide pool at Lajes do Pico, Azores. Boletim do Museu Municipal do Funchal 4: 55–64.Google Scholar
  6. Barreiros, J. P. & R. S. Santos, 1998. Notes on the food habits and predatory behavior of the dusky grouper, Epinephelus marginatus (Lowe, 1834) (Pisces: Serranidae) in the Azores, Arquipélago. Life and Marines Sciences 16: 29–35.Google Scholar
  7. Beckley, L. E., 2000. Species composition and recruitment of fishes to a tidal pool in KwaZulu-Natal, South Africa. African Zoology 35: 29–34.Google Scholar
  8. Begossi, A. & R. A. Silvano, 2008. Ecology and ethnoecology of dusky grouper [garoupa, Epinephelus marginatus (Lowe, 1834)] along the coast of Brazil. Journal of Ethnobiology and Ethnomedicine 4: 1–14.CrossRefGoogle Scholar
  9. Bergamino, L., D. Lercari & O. Defeo, 2011. Food web structure of sandy beaches: Temporal and spatial variation using stable isotope analysis. Estuarine, Coastal and Shelf Science 91: 536–543.CrossRefGoogle Scholar
  10. Bond, A. L. & A. W. Diamond, 2011. Recent Bayesian stable-isotope mixing models are highly sensitive to variation in discrimination factors. Ecological Applications 21: 1017–1023.PubMedCrossRefGoogle Scholar
  11. Bouillon, S., P. Chandra Mohan, N. Sreenivas & F. Dehairs, 2000. Sources of suspended organic matter and selective feeding by zooplankton in an estuarine mangrove eco-system as traced by stable isotopes. Marine Ecology Progress Series 208: 79–92.CrossRefGoogle Scholar
  12. Brulé, T., E. Puerto-Novelo, E. Pérez-Díaz & X. Renán-Galindo, 2005. Diet composition of juvenile black grouper (Mycteroperca bonaci) from coastal nursery areas of the Yucatán Peninsula, Mexico. Bulletin of Marine Science 77: 441–452.Google Scholar
  13. Bruslé, J., 1985. Expose synoptique des donnés biologiques sur les mérous Epinephelus aeneus (Geoffroy Saint Hilaire, 1809) et Epinephelus guaza (Linnaeus, 1758) de l’océan Atlantique et de le Méditerraneé. FAO Fisheries Synopsis 129: 1–64.Google Scholar
  14. Buchmann, F. S. C., M. Seeliger, L. Zanella, L. S. P. Madureira, L. J. Tomazelli & L. J. Calliari, 2001. Análise batimétrica e sedimentológica no estudo do Parcel do Carpinteiro, uma paleolinha de praia pleistocênica na antepraia do Rio Grande do Sul, Brasil. Pesquisas 28: 109–115.Google Scholar
  15. Canuel, E. A., J. E. Cloern, D. B. Ringelberg, J. B. Guckert & G. H. Rau, 1995. Molecular and isotopic tracers used to examine sources of organic matter and its incorporation into the food webs of San Francisco Bay. Limnology and Oceanography 40: 67–81.CrossRefGoogle Scholar
  16. Capítoli, R. R., 1996. Rubble structures and hard substrates. In Seeliger, U., C. Odebrecht & J. P. Castello (eds), Subtropical Convergence Environments: The Coast and Sea in the Southwestern Atlantic. Springer-Verlag, Berlim: 86–89.Google Scholar
  17. Caut, S., E. Angulo & F. Courchamp, 2009. Variation in discrimination factors (Delta N-15 and Delta C-13): the effect of diet isotopic values and applications for diet reconstruction. Journal of Applied Ecology 46: 443–453.CrossRefGoogle Scholar
  18. Claudino, M. C., P. C. Abreu & A. M. Garcia, 2013. Stable isotopes reveal temporal and between-habitat changes in trophic pathways in a southwestern Atlantic estuary. Marine Ecology Progress Series 489: 29–42.CrossRefGoogle Scholar
  19. Coleman, F. C., C. C. Koenig, G. R. Huntsman, J. A. Musick, A. M. Eklund, J. C. McGovern, R. W. Chapman, G. R. Sedberry & C. B. Grimes, 2000. Long-lived reef fishes: the grouper–snapper complex. Fisheries 25: 14–21.CrossRefGoogle Scholar
  20. Condini, M. V., A. M. Garcia & J. P. Vieira, 2007. Perfil do pescador e descrição da pesca da garoupa (Pisces, Serranidae) no Molhe Oeste da Barra de Rio Grande, RS. Pan-American Journal of Aquatic Sciences 2: 279–287.Google Scholar
  21. Condini, M. V., E. Seyboth, J. P. Vieira & A. M. Garcia, 2011. Diet and feeding strategy of the dusky grouper Mycteroperca marginata (Actinopterygii: Epinephelidae) in a man-made rocky habitat in southern Brazil. Neotropical Ichthyology 9: 161–168.CrossRefGoogle Scholar
  22. Condini, M. V., E. Seyboth, J. P. Vieira, A. S. Varela Jr, J. P. Barreiros, L. F. Fávaro & A. M. Garcia, 2013. First record of the dusky grouper Epinephelus marginatus (Actinopterygii: Epinephelidae) undergoing sexual transition in the South Western Atlantic, Brazil. Hidrobiológica 23: 448-45.Google Scholar
  23. Condini, M. V., L. F. Fávaro, A. S. Varela Jr & A. M. Garcia, 2014. Reproductive biology of the dusky grouper (Epinephelus marginatus) at the southern limit of its distribution in the south-western Atlantic. Marine and Freshwater Research 65: 142–152.CrossRefGoogle Scholar
  24. Connolly, R. M., D. Gorman & M. A. Guest, 2005. Movement of carbon among estuarine habitats and its assimilation by invertebrates. Oecologia 144: 684–691.PubMedCrossRefGoogle Scholar
  25. Cornish, A. & M. Harmelin-Vivien, 2004. Epinephelus marginatus. In 2006 IUCN Red List of Threatened Species [www.iucnredlist.org]. Accessed 5 December 2013.
  26. Craig, M. T., Y. J. Sadovy de Mitcheson & P. C. Heemstra, 2011. Groupers of the World: A Field and Market Guide. Boca Raton, Florida.Google Scholar
  27. Davis, A. M., M. L. Blanchette, B. J. Pusey, T. D. Jardine & R. G. Pearson, 2012. Gut content and stable isotope analyses provide complementary understanding of ontogenetic dietary shifts and trophic relationships among fishes in a tropical river. Freshwater Biology 57: 2156–2172.CrossRefGoogle Scholar
  28. Fennessy, Y. S. T., 2006. Reproductive biology and growth of the yellow-belly rockcod Epinephelus marginatus (Serranidae) from South-east Africa. African Journal of Marine Science 28: 1–11.CrossRefGoogle Scholar
  29. Figueiredo, J. L. & N. A. Menezes, 1980. Manual de peixes marinhos do sudeste do Brasil. III. Teleostei (2). Museu de Zoologia, Universidade de São Paulo, São Paulo.Google Scholar
  30. France, R. L., 1995. Differentiation between littoral and pelagic food webs in lakes using stable carbon isotopes. Limnology and Oceanography 40: 1310–1313.CrossRefGoogle Scholar
  31. Fry, B., 2006. Stable Isotope Ecology. Springer, New York.CrossRefGoogle Scholar
  32. Fry, B. & M. M. Chumchal, 2011. Sulfur stable isotope indicators of residency in estuarine fish. Limnology and Oceanography 56: 1563–1576.CrossRefGoogle Scholar
  33. Garcia, A. M., D. J. Hoeinghaus, J. P. Vieira & K. O. Winemiller, 2007. Isotopic variation of fishes in freshwater and estuarine zones of a large subtropical coastal lagoon. Estuarine, Coastal and Shelf Science 73: 399–408.CrossRefGoogle Scholar
  34. Grover, J. J., 1993. Trophic ecology of pelagic early-juvenile Nassau grouper, Epinephelus striatus, during an early phase of recruitment into demersal habitats. Bulletin of Marine Science 53: 1117–1125.Google Scholar
  35. Haimovici, M. & J. A. Álvarez-Pérez, 1990. Distribución y maduración sexual del calamar argentino, Illex argentinus (Castellanos, 1960) (Cephalopoda: Ommastrephidae), en el sur de Brasil. Scientia Marina 54: 179–185.Google Scholar
  36. Harmelin, J. & M. Harmelin-Vivien, 1999. A review on habitat, diet and growth of the dusky grouper Epinephelus marginatus (Lowe, 1834). Marine Life 9: 11–20.Google Scholar
  37. Harrod, C. G., J. Grey, T. K. McCarthy & M. Morrissey, 2005. Stable isotope analyses provide new insights into ecological plasticity in a mixohaline population of European eel. Oecologia 144: 673–683.PubMedCrossRefGoogle Scholar
  38. Heemstra, C. P. & J. E. Randall, 1993. FAO Species catalogue. Groupers of the word (Family Serranidae, Subfamily Epinephelinae, An Annotated and Illustred Catalogue of the Grouper, Rockcod, Hind, Coral grouper Lyretail Species. FAO Fisheries Synopsis 16: 186–189.Google Scholar
  39. Herrán, R. A., 1988. Analisis de contenidos estomacales em peces. Revision bibliografica de los objetivos y la metodologia. Informes Técnicos – Instituto Español de Oceanografía 63: 1–73.Google Scholar
  40. Hobson, K. A., 1999. Tracing origins and migration of wildlife using stable isotopes: a review. Oecologia 120: 314–326.CrossRefGoogle Scholar
  41. Hoeinghaus, D. J. & S. E. Davis III, 2007. Size-based trophic shifts of saltmarsh dwelling blue crabs elucidated by dual stable C and N isotope analyses. Marine Ecology Progress Series 334: 199–204.CrossRefGoogle Scholar
  42. Hoeinghaus, D. J., J. P. Vieira, C. S. Costa, C. E. Bemvenuti, K. O. Winemiller & A. M. Garcia, 2011. Estuary hydrogeomorphology affects carbon sources supporting aquatic consumers within and among ecological guilds. Hydrobiologia 673: 79–92.CrossRefGoogle Scholar
  43. Horta, P. A., F. Bucchmann, A. Souza, Z. L. Bouzon & E. C. Oliveira, 2008. Macroalgas do Parcel do Carpinteiro – com a adiação de Rhodymenia delicatula (Rhodophyta) à flora brasileira. Ínsula 37: 53–65.Google Scholar
  44. Huntsman, G. R., J. Potts, R. W. Mays & D. Vaughan, 1999. Groupers (Serranidae, Epinephelinae): Endangered apex predators of reef com-munities. In Musick, J. A. (ed.), Life in the Slow Lane: Ecology and Conservation of Long-lived Marine Animals. American Fisheries Society Symposium, Bethesda: 217–232.Google Scholar
  45. Hyslop, E. J., 1980. Stomach contents analysis – a review of methods and their application. Journal of Fish Biology 17: 411–429.CrossRefGoogle Scholar
  46. Ingram, T., B. Matthews, C. Harrod, T. Stephens, J. Grey, R. Markel & A. Mazumder, 2007. Lipid extraction has little effect on the δ15N of aquatic consumers. Limnology and Oceanography 5: 338–343.CrossRefGoogle Scholar
  47. Irigoyen, A. J., D. E. Galván & L. A. Venerus, 2005. Occurrence of dusky grouper Epinephelus marginatus (Lowe, 1834) in gulfs of northern Patagonia, Argentina. Journal of Fish Biology 67: 1741–1745.CrossRefGoogle Scholar
  48. Jardine, T.D., S. A. McGeachy, C. M. Paton, M. Savoie & R. A. Cunjak, 2003. Stable isotopes in aquatic systems: sample preparation, analysis, and interpretation. Canadian Manuscript Report of Fisheries and Aquatic Sciences No 265.Google Scholar
  49. Kaldy, J. E., L. A. Cifuentes & D. Brock, 2005. Using stable isotope analyses to assess carbon dynamics in a shallow subtropical estuary. Estuaries 28: 86–95.CrossRefGoogle Scholar
  50. Kulbicki, M., Y. Bozec, P. Labrosse, Y. Letourneur, G. Mou-Tham & L. Wantiez, 2005. Diet composition of carnivorous fishes from coral reef lagoons of New Caledonia. Aquatic Living Resource 18: 231–250.CrossRefGoogle Scholar
  51. Lembo, G., I. A. Flemming, F. Økland, P. Carbonara & M. T. Spedicato, 2002. Site fidelity of the dusky grouper Epinephelus marginatus (Lowe, 1834) studied by acoustic telemetry. Marine Life 9: 37–43.Google Scholar
  52. Linde, M., A. M. Grau, F. Riera & E. Massutí-Pascual, 2004. Analysis of trophic ontogeny in Epinephelus marginatus (Serranidae). Cybium 28: 27–35.Google Scholar
  53. López, V. G. & F. C. Orvay, 2005. Food habits of groupers Epinephelus marginatus (Lowe, 1834) and Epinephelus costae (Steindachner, 1878) in the Mediterranean Coast of Spain. Hidrobiológica 15: 27–34.Google Scholar
  54. Machado, L. M., F. A. M. L. Daros, A. A. Bertoncini, M. Hostim-Silva & J. P. Barreiros, 2008. Feeding strategy and trophic ontogeny in Epinephelus marginatus (Serranidae) from Southern Brazil. Cybium 32: 33–41.Google Scholar
  55. Manooch, C. S. & D. L. Mason, 1987. Age and growth of the warsaw grouper and black grouper from the southeast region of the United States. Northeast Gulf Science 9: 65–75.Google Scholar
  56. Marino, G., E. Azzurro, A. Massari, M. G. Finoia & A. Mandich, 2001. Reproduction is the dusky grouper from the southern Mediterranean. Journal of Fish Biology 58: 909–927.CrossRefGoogle Scholar
  57. McCarthy, I. D. & S. Waldron, 2000. Identifying migratory Salmo trutta using carbon and nitrogen stable isotope ratios. Rapid Communications in Mass Spectrometry 14: 1325–1331.PubMedCrossRefGoogle Scholar
  58. Möller, O. O., P. Casting, L. C. Salomon & P. Lazure, 2001. The influence of local and non-local forcing effects on the subtidal circulation of Patos Lagoon. Estuaries 24: 297–311.CrossRefGoogle Scholar
  59. Nelson, J., R. Wilson, F. Coleman, C. Koenig, D. De Vries, C. Gardner & J. Chanton, 2012. Flux by fin: fish-mediated carbon and nutrient flux in the northeastern Gulf of Mexico. Marine Biology 159: 365–372.CrossRefGoogle Scholar
  60. Oksanen, J., F. G. Blanchet, R. Kindt, P. Legendre, P. R. Minchin, R. B. O’Hara, G. L. Simpson, P. Solymos, M. H. H. Stevens & H. Wagner. 2013. Vegan: Community Ecology Package. R package version 2.0-9 [http://CRAN.R-project.org/package=vegan].
  61. Oliveira, M. C. L. M., R. F. Bastos, M. C. Claudino, C. M. Assumpção & A. M. Garcia, 2014. Transport of marine-derived nutrients to subtropical freshwater food webs by juvenile mullets: a case study in southern Brazil. Aquatic Biology 20: 91–100.CrossRefGoogle Scholar
  62. Parrish, J. D., 1987. The trophic biology of snappers and groupers. In Polovina, J. J. & S. Ralston (eds), Tropical Snappers and Groupers: Biology and Fisheries Management. Westview Press, Boulder, CO: 405–463.Google Scholar
  63. Pastor, J., M. Verdoit-Jarraya, P. Astruch, N. Dalias, J. S. N. Pasqual, G. Saragoni & P. Lenfant, 2009. Acoustic telemetry survey of the dusky grouper (Epinephelus marginatus) in the Marine Reserve of Cerbère-Banyuls: informations on the territoriality of this emblematic species. C. R. Biologies 332: 732–740.PubMedCrossRefGoogle Scholar
  64. Peterson, B. J. & B. Fry, 1987. Stable isotopes in ecosystem studies. Annual Review of Ecology and Systematics 18: 293–320.CrossRefGoogle Scholar
  65. Peterson, B. J., B. Fry, M. A. Hullar, S. Saupe & R. Wright, 1994. The distribution and stable carbon isotopic com-position of dissolved organic carbon in estuaries. Estuaries 17: 111–121.CrossRefGoogle Scholar
  66. Pinkas, L., M. S. Oliphant & I. L. K. Iverson, 1971. Food habits of albacore, bluefin tuna, and bonito in California waters. Fish Bulletin 152: 1–105.Google Scholar
  67. Post, D. M., 2002. Using stable isotopes to estimate trophic position: models, methods, and assumptions. Ecology 83: 703–718.CrossRefGoogle Scholar
  68. Post, D. M., C. A. Layman, D. A. Arrington, G. Takimoto, J. Quattrochi & C. G. Montaña, 2007. Getting to the fat of the matter: models, methods and assumptions for dealing with lipids in stable isotope analyses. Oecologia 152: 179–189.PubMedCrossRefGoogle Scholar
  69. Reñones, O., N. V. C. Polunin & R. Goni, 2002. Size related dietary shifts of Epinephelus marginatus in a western Mediterranean littoral ecosystem: an isotope and stomach content analysis. Journal of Fish Biology 61: 122–137.CrossRefGoogle Scholar
  70. Reñones, O., A. Grau, X. Mas, F. Riera & F. Saborido-Rey, 2010. Reproductive pattern of an exploited dusky grouper Epinephelus marginatus (Lowe 1834) (Pisces: Serranidae) population in the western Mediterranean. Scientia Marina 74: 523–537.CrossRefGoogle Scholar
  71. Sadovy de Mitcheson, Y., M. T. Craig, A. A. Bertoncini, K. E. Carpenter, W. W. L. Cheung, J. H. Choat, A. S. Cornish, S. T. Fennessy, B. P. Ferreira, P. C. Heemstra, M. Liu, R. F. Myers, D. A. Pollard, K. L. Rhodes, L. A. Rocha, B. C. Russell, M. A. Samoilys & J. Sanciangco, 2013. Fishing groupers towards extinction: a global assessment of threats and extinction risks in a billion dollar fishery. Fish and fisheries 14: 119–136.CrossRefGoogle Scholar
  72. Santos, R. A. & M. Haimovici, 2002. Cephalopods in the trophic relations off southern Brazil. Bulletin of Marine Science 71: 753–770.Google Scholar
  73. Seyboth, E., M. V. Condini, C. Q. Albuquerque, A. S. Varela Jr, G. Velasco, J. P. Vieira & A. M. Garcia, 2011. Age, growth, and reproductive aspects of the dusky grouper Mycteroperca marginata (Actinopterygii: Epinephelidae) in a man-made rocky habitat in southern Brazil. Neotropical Ichthyology 9: 849–856.CrossRefGoogle Scholar
  74. Sluka, R. D., M. Chiappone & K. M. Sullivan Sealey, 2001. Influence of habitat on grouper abundance in the Florida Keys, USA. Journal of Fish Biology 58: 682–700.CrossRefGoogle Scholar
  75. Vanderklift, M. A. & S. Ponsard, 2003. Sources of variation in consumer-diet d15N enrichment: a meta-analysis. Oecologia 136: 169–182.PubMedCrossRefGoogle Scholar
  76. Vieira, J. P., 1991. Juvenile mullets (Pisces: Mugilidae) In the Estuary of Lagoa dos Patos, RS - Brazil. Copeia 2: 409–418.CrossRefGoogle Scholar
  77. Winemiller, K. O., 2005. Life history strategies, population regulation, and implications for fisheries management. Canadian Journal of Fisheries and Aquatic Sciences 62: 872–885.CrossRefGoogle Scholar
  78. Winemiller, K. O., S. C. Zeug, C. R. Robertson, B. K. Winemiller & R. L. Honeycutt, 2011. Food-web structure of coastal streams in Costa Rica revealed by dietary and stable isotope analyses. Journal of Tropical Ecology 27: 463–476.CrossRefGoogle Scholar
  79. Wyatt, A. S. J., A. M. Waite & S. Humphries, 2010. Variability in isotope discrimination factors in coral reef fishes: implications for diet and food web reconstruction. Plos One 5(10): 1–10.CrossRefGoogle Scholar
  80. Zabala, M., P. Louisy, A. Garcia-Rubies & V. Gracia, 1997. Socio-behavioral context of reproduction in the Mediterranean dusky grouper Epinephelus marginatus (Lowe, 1834) (Pisces, Serranidae) in the Medes Islands Marine Reserve (NW Mediterranean, Spain). Scientia Marina 61: 78–79.Google Scholar
  81. Zar, J. H., 1994. Biostatistical Analysis, 2ª ed. Prentice Hall, Upper saddle river.Google Scholar

Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Mario V. Condini
    • 1
  • David J. Hoeinghaus
    • 2
  • Alexandre M. Garcia
    • 1
  1. 1.Laboratory of Ichthyology, Institute of OceanographyFederal University of Rio GrandeRio GrandeBrazil
  2. 2.Department of Biological Sciences and the Institute of Applied SciencesUniversity of North TexasDentonUSA

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