, Volume 722, Issue 1, pp 183–198 | Cite as

Evidence for consumer regulation of biofilm–nutrient interactions among hardwater streams (Pennsylvania, USA)

Primary Research Paper


Experimental studies evaluating the simultaneous effects of consumers, nutrients, and other biotic/abiotic factors on intact, natural food webs are rare, particularly among ecosystems of varying trophic conditions. We conducted a series of in situ studies that used nutrient-diffusing substrata with nitrogen (N) and phosphorus (P) concentrations in a full factorial design in three temperate, limestone streams in Pennsylvania across a trophic gradient (mesotrophic, eutrophic, and hypereutrophic streams). We assessed differences in algal and macroinvertebrate biomass, taxonomic composition, and functional groups relative to amended nutrients across the trophic gradient; as such, these results facilitated predictions about regulators of food web structure. All factors varied significantly among the streams (e.g., algal biomass P = 0.005, macroinvertebrate biomass P < 0.001, algal diversity P = 0.006, macroinvertebrate diversity P < 0.001, algal group P < 0.001, macroinvertebrate guilds P < 0.001); the streams, however, did not exhibit simple responses to nutrient amendment. Algal and macroinvertebrate biomass and diversity responded greatest in the mesotrophic stream while grazing seemed to be a strong factor preventing algal nutrient response in the eutrophic and hypereutrophic streams. Brillouin’s Evenness Index was most influenced by nutrient amendment (nutrient effect on algae and macroinvertebrates P = 0.021). As such, we concluded that biomass and diversity were mediated by complexity within intermediate trophic levels.


Diversity Macroinvertebrate Grazing Algae Chironomidae 



The authors would like to thank J. Stauffer and G. Hoover for constructive feedback and suggestions on this work as well as K. Price for comments on a previous version of this manuscript. Funding for this project was provided by the Pennsylvania Department of Environmental Protection Grant 4300227693. The paper is contribution number 33, Institute for Great Lakes, Central Michigan University.


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© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Department of Ecosystem Science and ManagementThe Pennsylvania State UniversityUniversity ParkUSA
  2. 2.Department of Biology & Institute for Great Lakes ResearchCentral Michigan UniversityMount PleasantUSA

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