, Volume 692, Issue 1, pp 67–81 | Cite as

Nutrient addition retards decomposition and C immobilization in two wet grasslands

  • Eva Kaštovská
  • Tomáš Picek
  • Jiří Bárta
  • Jiří Mach
  • Tomáš Cajthaml
  • Keith Edwards


Eutrophication is one of the biggest environmental problems facing wetlands. However, its effect on soil functioning is not yet well understood. We tested the hypothesis that increased nutrient loading into wet grassland ecosystems accelerates soil C and N cycles and decreases microbial immobilization of C and N. Experimental sites were established on two wet grasslands, with either mineral or peaty soils, and fertilized by NPK fertilizer for 3 years. Soils were analyzed for soluble and microbial C and N contents and their transformations, profile of phospholipid fatty acids and number of nirK denitrifiers. Fertilization affected C more than N transformations. Opposite to what was predicted, decomposition was retarded, the soil C cycle was based more on labile C compounds, and the soil was more susceptible to C losses in fertilized versus unfertilized treatments in both soils. Fertilization resulted in lower microbial biomass C and microbial C immobilization and also decreased the activity of lignin-degrading enzymes. Shifts in the composition of the microbial communities led to decreased (1) decomposition of complex organic compounds and (2) immobilization of transformed C. Net nitrification and microbial N immobilization tended to increase in fertilized treatments indicating an acceleration of soil N cycling and losses, but only in the more vulnerable organic soil.


Wet meadows Eutrophication Lignin-degrading enzymes qPCR 



This study was supported by Projects No. 526/09/1545 (awarded to Dr. Keith Edwards), No. 526/08/0751 of the Grant Agency of the Czech Republic and No. GAJU04-142/2010/P of the Grant Agency of University of South Bohemia. We also thank our technician Terezia Říhová, and our students Pavla Staňková and Veronika Špátová, for their perfect work on the project.


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Eva Kaštovská
    • 1
  • Tomáš Picek
    • 1
  • Jiří Bárta
    • 1
  • Jiří Mach
    • 1
  • Tomáš Cajthaml
    • 2
  • Keith Edwards
    • 1
  1. 1.Department of Ecosystem Biology, Faculty of ScienceUniversity of South BohemiaCeske BudejoviceCzech Republic
  2. 2.Laboratory of Environmental MicrobiologyInstitute of Microbiology, Academy of Science Czech Republic, VviPrague 4Czech Republic

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