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Human urine-derived stem cells play a novel role in the treatment of STZ-induced diabetic mice

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Abstract

Human urine-derived stem cells (hUSCs) are a potential stem cell source for cell therapy. However, the effect of hUSCs on glucose metabolism regulation in type 1 diabetes was not clear. Therefore, the aim of the study was to evaluate whether hUSCs have protective effect on streptozotocin (STZ)-induced diabetes. hUSCs were extracted and cultivated with a special culture medium. Flow cytometry analysis was applied to detect cell surface markers. BALB/c male nude mice were either injected with high-dose STZ (HD-STZ) or multiple low-dose STZ (MLD-STZ). Serum and pancreatic insulin were measured, islet morphology and its vascularization were investigated. hUSCs highly expressed CD29, CD73, CD90 and CD146, and could differentiate into, at least, bone and fat in vitro. Transplantation of hUSCs into HD-STZ treated mice prolonged the median survival time and improved their blood glucose, and into those with MLD-STZ improved the glucose tolerance, islet morphology and increased the serum and pancreas insulin content. Furthermore, CD31 expression increased significantly in islets of BALB/c nude mice treated with hUSCs compared to those of un-transplanted MLD-STZ mice. hUSCs could improve the median survival time and glucose homeostasis in STZ-treated mice through promoting islet vascular regeneration and pancreatic beta-cell survival.

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Acknowledgements

This study was supported by Shanghai Nature Science Fund (16ZR1425800). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Author notes

  1. Tianxue Zhao and Deng Luo contributed equally to this work.

Authors and Affiliations

  1. Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Diabetes Institute, Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 600 Yishan Road, Shanghai, 200233, People’s Republic of China

    Tianxue Zhao, Deng Luo, Yun Sun, Chen Wang & Weiping Jia

  2. Institute of Microsurgery on Extremities, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 600 Yishan Road, Shanghai, 200233, People’s Republic of China

    Xin Niu & Yang Wang

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  1. Tianxue Zhao
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  2. Deng Luo
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  4. Xin Niu
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  5. Yang Wang
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  6. Chen Wang
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Correspondence to Chen Wang.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the Ethics Committee of Shanghai Jiao Tong University Affiliated Six People’s Hospital and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Animal studies were approved by the Animal Care Committee of Shanghai Jiao Tong University Affiliated Six People’s Hospital. All animals received human care in accordance with the Guidelines for the Care and Use of Laboratory Animals (NIH publication No. 86-23, revised 1985).

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Zhao, T., Luo, D., Sun, Y. et al. Human urine-derived stem cells play a novel role in the treatment of STZ-induced diabetic mice. J Mol Hist 49, 419–428 (2018). https://doi.org/10.1007/s10735-018-9772-5

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