A zebrafish mosaic assay to study mammalian stem cells in real time in vivo
The differentiation potentials of stem cells have been evaluated by various in vivo and in vitro assays. However, these assays have different limitations hindering efficient study of mammalian stem cells. Here we describe a rapid and powerful mosaic assay to study the differentiation potentials of stem cells in real time in vivo by using zebrafish embryo. We transplanted mouse neural stem cells into zebrafish embryos at different developmental stages and found that they mainly formed neural tissues while occasionally trans-differentiated into mesoderm- and endoderm-derived tissues. Because zebrafish embryo is transparent, the behaviors of transplanted mouse stem cells can be easily tracked in a real-time manner and at single-cell resolution. We expect that this assay may be widely applied to explore the in vivo behaviors of any stem cells available.
KeywordsZebrafish chimera Transplantation Neural stem cell Trans-differentiation
This work was funded by the National Natural Science Foundation of China (Nos. 81372143 and 31000646).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflictof interest.
- Westerfield M (2000) The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio), 4th edn. University of Oregon Press, EugeneGoogle Scholar