Odontoblastic inductive potential of epithelial cells derived from human deciduous dental pulp
- 399 Downloads
For the dentin regeneration, dental epithelial cells are indispensible and must possess odontoblastic induction capability. Epithelial cell-like stem cells were recently identified in human deciduous dental pulp (DPESCs). However, their cellular characteristics remain poorly defined. The purpose of this study was to characterize DPESCs compared to HAT-7 ameloblastic cells. Expression levels of ameloblast-specific markers [odontogenic ameloblast-associated protein (Odam), matrix metalloproteinase (Mmp)-20, amelogenin, and ameloblastin] were detected in DPESCs. Co-culturing odontoblastic MDPC-23 cells with DPESCs increased expression of odontoblast differentiation markers (Dmp1 and Dspp) from days 4 to 10, while the expression of bone sialoprotein rapidly decreased. MDPC-23 cells cultured in DPESC-conditioned medium (CM) showed increased Dspp promoter activity compared with control MDPC-23 cultures. Mineralization was first observed in the CM groups from day 4 and proceeded rapidly until day 14, whereas mineralized nodules were found from day 7 in control media-cultured cells. In conclusion, DPESCs in human deciduous pulp possess ameloblast-like characteristics and differentiation properties, and substances derived from DPESCs promote odontoblastic differentiation. Thus, our results indicate that DPESCs can be a realistic epithelial source for use in odontoblastic induction and dentin formation of dental mesenchymal cells.
KeywordsDental epithelial cell Deciduous pulp Induction Epithelial-mesenchymal interaction Odontoblast
This research was supported by the Bio and Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MSIP) (No. NRF-2013M3A9B2076480).
- Ikeda E, Morita R, Nakao K, Ishida K, Nakamura T, Takano-Yamamoto T, Ogawa M, Mizuno M, Kasugai S, Tsuji T (2009) Fully functional bioengineered tooth replacement as an organ replacement therapy. Proc Natl Acad Sci USA 106:13475–13480. doi: 10.1073/pnas.0902944106 CrossRefPubMedPubMedCentralGoogle Scholar
- Iohara K, Zheng L, Ito M, Tomokiyo A, Matsushita K, Nakashima M (2006) Side population cells isolated from porcine dental pulp tissue with self-renewal and multipotency for dentinogenesis, chondrogenesis, adipogenesis, and neurogenesis. Stem Cells 24:2493–2503. doi: 10.1634/stemcells.2006-0161 CrossRefPubMedGoogle Scholar
- Ishimatsu H, Kitamura C, Morotomi T, Tabata Y, Nishihara T, Chen KK, Terashita M (2009) Formation of dentinal bridge on surface of regenerated dental pulp in dentin defects by controlled release of fibroblast growth factor-2 from gelatin hydrogels. J Endod 35:858–865. doi: 10.1016/j.joen.2009.03.049 CrossRefPubMedGoogle Scholar
- Lee DS, Park JT, Kim HM, Ko JS, Son HH, Gronostajski RM, Cho MI, Choung PH, Park JC (2009) Nuclear factor I-C is essential for odontogenic cell proliferation and odontoblast differentiation during tooth root development. J Biol Chem 284:17293–17303. doi: 10.1074/jbc.M109.009084 CrossRefPubMedPubMedCentralGoogle Scholar
- Lee HK, Park JT, Cho YS, Bae HS, Cho MI, Park JC (2012) Odontogenic ameloblasts-associated protein (ODAM), via phosphorylation by bone morphogenetic protein receptor type IB (BMPR-IB), is implicated in ameloblast differentiation. J Cell Biochem 113:1754–1765. doi: 10.1002/jcb.24047 PubMedGoogle Scholar