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Journal of Molecular Histology

, Volume 47, Issue 2, pp 135–143 | Cite as

IGF1 3′UTR functions as a ceRNA in promoting angiogenesis by sponging miR-29 family in osteosarcoma

  • Shuming Gao
  • Cai Cheng
  • Hanwen Chen
  • Min Li
  • Kehun Liu
  • Guangya Wang
Original Paper

Abstract

Osteosarcoma is one of the most common malignant bone tumors in human worldwide. Angiogenesis is a pivotal process during osteosarcoma development. Insulin-like growth factor 1 (IGF1) has been reported to promote angiogenesis. However, the role of 3′ untranslational region (3′UTR) of IGF1 mRNA in angiogenic activity in osteosarcomas is still unknown. In the present study, we performed gain-of-function assays to investigate the role of IGF1-3′UTR in angiogenesis. For the first time, we demonstrated that IGF1 3′UTR increased VEGF expression and promotes angiogenesis in osteosarcoma cells. In addition, RNA-immunoprecipitation and luciferase reporter assays showed that IGF1 3′UTR was a direct target of miR-29s. Our data also demonstrated that there existed a competition of miR-29s between IGF1-3′UTR and VEGF mRNA, and IGF1-3′UTR promoted angiogenesis at least in part via sponging miR-29s. Taken together, our study suggests that IGF1-3′UTR functions as a ceRNA in promoting angiogenesis by sponging miR-29s in osteosarcoma.

Keywords

Osteosarcoma IGF1 ceRNA VEGF miR-29a miR-29b miR-29c Angiogenesis 

Notes

Acknowledgments

Thanks for the contribution of all authors.

Compliance with ethical standards

Conflict of interest

None.

Supplementary material

10735_2016_9659_MOESM1_ESM.xlsx (12 kb)
Supplementary material 1 (XLSX 12 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Shuming Gao
    • 1
  • Cai Cheng
    • 1
  • Hanwen Chen
    • 1
  • Min Li
    • 1
  • Kehun Liu
    • 1
  • Guangya Wang
    • 1
  1. 1.Department of orthopaedicsCangzhou Central HospitalCangzhouChina

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