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Expression and localization of laminin 5, laminin 10, type IV collagen, and amelotin in adult murine gingiva

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Abstract

The biochemical composition of the internal and external basal laminae in the junctional epithelium differs significantly, and the precise cellular origin of their respective molecules remains to be determined. In the present study, the expression and localization of three basement membrane-specific molecules—laminin 5 (γ2 chain), type IV collagen (α1 chain), and laminin 10 (α5 chain)—and one tooth-specific molecule, amelotin, was analyzed in adult murine gingiva by using in situ hybridization and immunohistochemistry. The results showed that the outermost cells in junctional epithelium facing the tooth enamel strongly expressed laminin 5 mRNA, supporting the immunohistochemical staining data. This suggests that laminin 5 is actively synthesized in junctional epithelial cells and that the products are incorporated into the internal basal lamina to maintain firm epithelial adhesion to the tooth enamel throughout life. Conversely, no amelotin mRNA signals were detected in the junctional epithelial cells, suggesting that the molecules localized on the internal basal lamina are mainly derived from maturation-stage ameloblasts. Weak and sporadic expression of type IV collagen in addition to laminin 10 in the gingiva indicates that these molecules undergo turnover less frequently in adult animals.

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Acknowledgments

We are grateful to Dr. Ganss, University of Toronto, for AMTN antibody, and Professor Jeremy Williams, Tokyo Medical University, for his assistance with the English of this manuscript. This work was supported by a Grant-in-Aid for Scientific Research (24592779; T.S.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Department of Ultrastructural Science, Tokyo Dental College, Misaki-cho 2-9-18, Chiyoda-ku, Tokyo, 101-0061, Japan

    Takashi Sawada & Takaki Yamazaki

  2. Department of Microbiology, Tokyo Dental College, Misaki-cho 2-9-18, Chiyoda-ku, Tokyo, 101-0061, Japan

    Kazuko Shibayama

  3. Department of Pediatric Dentistry, Tokyo Dental College, Misaki-cho 2-9-18, Chiyoda-ku, Tokyo, 101-0061, Japan

    Kaido Kumazawa

  4. Department of Biochemistry, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan

    Yoko Yamaguchi

  5. Department of Biochemistry, Ohu University School of Pharmaceutical Sciences, Misumido 31-1, Tomita-machi, Koriyama, Fukushima, 963-8611, Japan

    Mitsuhiro Ohshima

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  1. Takashi Sawada
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  2. Takaki Yamazaki
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  3. Kazuko Shibayama
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  4. Kaido Kumazawa
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Correspondence to Takashi Sawada.

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Sawada, T., Yamazaki, T., Shibayama, K. et al. Expression and localization of laminin 5, laminin 10, type IV collagen, and amelotin in adult murine gingiva. J Mol Hist 45, 293–302 (2014). https://doi.org/10.1007/s10735-013-9559-7

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  • DOI: https://doi.org/10.1007/s10735-013-9559-7

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