Journal of Molecular Histology

, Volume 45, Issue 3, pp 293–302 | Cite as

Expression and localization of laminin 5, laminin 10, type IV collagen, and amelotin in adult murine gingiva

  • Takashi SawadaEmail author
  • Takaki Yamazaki
  • Kazuko Shibayama
  • Kaido Kumazawa
  • Yoko Yamaguchi
  • Mitsuhiro Ohshima
Original Paper


The biochemical composition of the internal and external basal laminae in the junctional epithelium differs significantly, and the precise cellular origin of their respective molecules remains to be determined. In the present study, the expression and localization of three basement membrane-specific molecules—laminin 5 (γ2 chain), type IV collagen (α1 chain), and laminin 10 (α5 chain)—and one tooth-specific molecule, amelotin, was analyzed in adult murine gingiva by using in situ hybridization and immunohistochemistry. The results showed that the outermost cells in junctional epithelium facing the tooth enamel strongly expressed laminin 5 mRNA, supporting the immunohistochemical staining data. This suggests that laminin 5 is actively synthesized in junctional epithelial cells and that the products are incorporated into the internal basal lamina to maintain firm epithelial adhesion to the tooth enamel throughout life. Conversely, no amelotin mRNA signals were detected in the junctional epithelial cells, suggesting that the molecules localized on the internal basal lamina are mainly derived from maturation-stage ameloblasts. Weak and sporadic expression of type IV collagen in addition to laminin 10 in the gingiva indicates that these molecules undergo turnover less frequently in adult animals.


In situ hybridization Immunohistochemistry Laminins Type IV collagen Amelotin Gingiva 



We are grateful to Dr. Ganss, University of Toronto, for AMTN antibody, and Professor Jeremy Williams, Tokyo Medical University, for his assistance with the English of this manuscript. This work was supported by a Grant-in-Aid for Scientific Research (24592779; T.S.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Takashi Sawada
    • 1
    Email author
  • Takaki Yamazaki
    • 1
  • Kazuko Shibayama
    • 2
  • Kaido Kumazawa
    • 3
  • Yoko Yamaguchi
    • 4
  • Mitsuhiro Ohshima
    • 5
  1. 1.Department of Ultrastructural ScienceTokyo Dental CollegeChiyoda-kuJapan
  2. 2.Department of MicrobiologyTokyo Dental CollegeChiyoda-kuJapan
  3. 3.Department of Pediatric DentistryTokyo Dental CollegeChiyoda-kuJapan
  4. 4.Department of BiochemistryNihon University School of DentistryChiyoda-kuJapan
  5. 5.Department of BiochemistryOhu University School of Pharmaceutical SciencesKoriyamaJapan

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