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Up-regulated expression of Bnip3L after intracerebral hemorrhage in adult rats

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Abstract

Bnip3L, also known as NIX, is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3 which can bind to Bcl-2 to elaborate that effect. In tumor cells, Bnip3L played a role in tumor growth inhibition, but some studies argued hypoxia-induced autophagy via Bnip3L was a survival mechanism that promoted tumor progression. In heart muscle, it related to decreased myocardial function. However, its function in intracerebral hemorrhage (ICH) is still not clear. In this frame, we found the Bnip3L expression increased in the perihematomal region in adult rats after performed ICH. Double immunofluorenscence staining manifested that Bnip3L co-located with neurons, not astrocytes or oligodendrocytes. Furthermore, we detected that neuronal apoptosis marker active caspase-3 had colocalizations with Bnip3L. In addition, colocalizations and co-immunoprecipitation between Bnip3L and Bcl-2, consistent with previous study, were also found. All our findings suggested that Bnip3L might be involved in the pathophysiology of ICH.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81070992) and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People’s Republic of China

    Ying Rui, Kaifu Ke, Lei Li, Heyi Zheng, Wei Xu, Xiang Tan, Jianhua Cao, Xiaoyan Wu, Gang Cui, Guangwei Zhao, Yilu Gao & Maohong Cao

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  1. Ying Rui
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  2. Kaifu Ke
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  3. Lei Li
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  4. Heyi Zheng
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  5. Wei Xu
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  7. Jianhua Cao
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  8. Xiaoyan Wu
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  9. Gang Cui
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  10. Guangwei Zhao
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  11. Yilu Gao
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Correspondence to Yilu Gao or Maohong Cao.

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Ying Rui and Kaifu Ke contributed equally to this work.

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Rui, Y., Ke, K., Li, L. et al. Up-regulated expression of Bnip3L after intracerebral hemorrhage in adult rats. J Mol Hist 44, 497–505 (2013). https://doi.org/10.1007/s10735-013-9506-7

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  • DOI: https://doi.org/10.1007/s10735-013-9506-7

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