Journal of Molecular Histology

, Volume 44, Issue 3, pp 347–356 | Cite as

Increased PELP1 expression in rat periodontal ligament tissue in response to estrogens treatment

Original Paper


Estrogens and their receptors are important factors involved in periodontal ligament (PDL) tissue health. As a regulator of estrogen receptors (ER), the proline-, glutamic acid-, and leucine-rich protein 1 (PELP1) may play a role in alveolar bone formation and PDL homeostasis. The aim of the present study was to observe PELP1 expression in rat PDL tissue during estrogen levels manipulations. Twenty-one 8-week old normal female Sprague–Dawley rats were randomly divided into three equal groups: sham-operated controls, ovariectomized (OVX) group, and OVX given 17β-estradiol intraperitoneally (OVX + E2) for 16 weeks. PELP1 expression was down-regulated in the OVX group and was up-regulated in the OVX + E2 group. Periodontal ligament fibroblast cells (PDLFCs) were isolated from PDL tissue, and characterized by immunohistochemical staining. Estradiol treatment of PDLFCs induced PELP1 protein level compared to untreated cells. PELP1 mRNA expression in estradiol-treated cells was relatively low at the beginning of treatment and then steadily increased from hour 4. In conclusion, results indicate that PELP1 is expressed in rat PDL tissue and PDLFCs, and that its expression is up-regulated during estrogen treatment.


Estrogen PELP1 Ovariectomized rats Periodontal ligament 



The authors gratefully acknowledge the support of 306 Hospital of PLA. We thank the staff in the Center of Pathology and Experimental Medicine of 306 Hospital of PLA for their skills, knowledge and advice.

Conflict of interest

No potential conflicts of interest were disclosed.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Department of Orthodontics, College of StomatologyForth Military Medical UniversityXi’anChina
  2. 2.Department of UrologicalGeneral Hospital of People’s Liberation ArmyBeijingChina
  3. 3.Department of Pathology and Experimental MedicineBeijingChina
  4. 4.Department of Plastic SurgeryGeneral Hospital of People’s Liberation ArmyBeijingChina

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