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Salivary gland expression level of IκBα regulatory protein in Sjögren’s syndrome

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Abstract

Diagnosis and therapeutic strategies in Sjögren’s syndrome (SS) might greatly benefit of the present multidisciplinary approach to studying the molecular pathogenesis of the disease. A deregulated inflammatory response has been described in the SS. The research in the last years sheds light on the importance of the NF-κB pathway regulating the pro-inflammatory cytokine production and leukocyte recruitment. These are important contributors to the inflammatory response during the development of SS. In this study we examine the expression of the NF-κB inhibitory protein termed IκBα in salivary glands epithelial cells (SGEC) comparing it with SGEC from healthy controls, to test the hypothesis that an altered expression of IκBα occurs in SGEC from SS biopsies. Real-Time PCR, western blot and immunohistochemistry demonstrated that the expression level of IκBα was significantly lower in SS with respect to healthy controls leading to an increased NF-κB activity. Our results suggest that the analysis of IκBα expression at salivary gland epithelial cell level could be a potential new hallmark of SS progression and sustain a rationale to more deeply investigate the therapeutic potential of specific NF-κB inhibitors in SS.

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Acknowledgments

We are grateful to M.V.C. Pragnell, B.A., for critical reading of the manuscript.

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Authors and Affiliations

  1. Laboratory of Cell Biology, Section of Human Anatomy and Histology, Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari Medical School, piazza Giulio Cesare 1, 70124, Bari, Italy

    Margherita Sisto, Sabrina Lisi & Raffaella De Lucro

  2. Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy

    Dario Domenico Lofrumento

  3. Department of Pathology, University of Bari Medical School, Bari, Italy

    Giuseppe Ingravallo

  4. Section of Rheumatology, Department of Internal Medicine and Public Medicine, University of Bari Medical School, Bari, Italy

    Massimo D’Amore

Authors
  1. Margherita Sisto
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  2. Sabrina Lisi
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  3. Dario Domenico Lofrumento
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  4. Giuseppe Ingravallo
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  5. Raffaella De Lucro
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  6. Massimo D’Amore
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Correspondence to Margherita Sisto.

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Margherita Sisto and Sabrina Lisi have equal contribution in this work and both are equally considered as “first author”.

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Sisto, M., Lisi, S., Lofrumento, D.D. et al. Salivary gland expression level of IκBα regulatory protein in Sjögren’s syndrome. J Mol Hist 44, 447–454 (2013). https://doi.org/10.1007/s10735-013-9487-6

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  • DOI: https://doi.org/10.1007/s10735-013-9487-6

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