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Increased expression of nitric oxide synthase interacting protein (NOSIP) following traumatic spinal cord injury in rats

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Abstract

Previous studies indicated that nitric oxide (NO) is involved in secondary damage of spinal cord injury (SCI), which worsens the primary physical injury to the central nervous systems. Recently, nitric oxide synthase interacting protein (NOSIP) has been identified to interact with neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase by inhibiting the NO production. However, its expression and function after a central nervous system injury remains unclear. In this study, we examined the expression and cellular localization of NOSIP in the spinal cord of an adult rat. Western blot analysis indicated that NOSIP protein levels increased at day1 post-injury and peaked at day 14. Double immunofluorescence staining showed that NOSIP was primarily expressed in neurons and glial cells in the intact spinal cord. Interestingly, this study also showed that the expression of NOSIP significantly increased in astrocytes after injury. Furthermore, injury-induced expression of NOSIP was co-expressed with proliferating cell nuclear antigen (PCNA) positive astrocytes after injury. We also showed the NOSIP was co-localized with nNOS in gray matter and white matter after SCI. All these data taken together suggested that NOSIP may play an important roles in astrogliogenesis after a spinal cord injury.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81071487, No. 31070723, and No. 31270802); Natural Science Foundation of Jiangsu Province, China (No. BK2009449); Social Development Program of Jiangsu Province, China (No. BE2010744); and Medical Scientific Research Foundation of Jiangsu Province, China (No. H201008).

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Authors and Affiliations

  1. Department of Orthopaedics, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, 210011, China

    Xiaowei Yu, Yi Zhong, Tianyi Wu & Ye Huang

  2. Department of Geriatrics, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, 210011, China

    Aiguo Shen

  3. Department of Orthopaedics, Shanghai Sixth People’s Hospital, Shanghai Jiaotong University, Shanghai, 200233, China

    Xiaowei Yu & Zhenzhong Zhu

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  1. Xiaowei Yu
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  2. Yi Zhong
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Correspondence to Aiguo Shen or Ye Huang.

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Xiaowei Yu and Yi Zhong contributed equally to this work.

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Yu, X., Zhong, Y., Zhu, Z. et al. Increased expression of nitric oxide synthase interacting protein (NOSIP) following traumatic spinal cord injury in rats. J Mol Hist 43, 661–668 (2012). https://doi.org/10.1007/s10735-012-9460-9

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