Protective effects of Lycium barbarum polysaccharide on neonatal rat primary cultured hippocampal neurons injured by oxygen-glucose deprivation and reperfusion
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This study investigated the protective effects of Lycium barbarum polysaccharide (LBP) on alleviating injury from oxygen-glucose deprivation/reperfusion (OGD/RP) in primary cultured rat hippocampal neurons. Cultured hippocampal neurons were exposed to oxygen-glucose deprivation (OGD) for 2 h followed by a 24 h re-oxygenation. The MTT assay and the lactate dehydrogenase (LDH) release were used to determine the neuron viability. Superoxide dismutase (SOD), Glutathione peroxidase (GSH-PX), malondialdehyde (MDA) were determined by spectrophotometry using commercial kits. Mitochondrial membrane potential (MMP) and the intracellular free calcium concentration ([Ca2+]i) in hippocampal neurons were measured using the confocal laser scanning microscope (CLSM). Treatment with LBP (10–40 mg/l) significantly attenuated neuronal damage and inhibited LDH release in a dose-dependent manner. Furthermore, LBP enhanced activities of SOD and GSH-PX but it decreased their MDA content, inhibited [Ca2+]i elevation and decrease of MMP in ischemia–reperfusion treated hippocampal neurons. These findings suggested that LBP may be a potential neuroprotective agent for cerebral ischemia–reperfusion injury.
KeywordsLycium barbarum polysaccharide (LBP) Hippocampal neurons Oxygen-glucose deprivation and reperfusion Neuroprotection
The study was supported by the National Natural Science Foundation of China (Grant No. 30960506, 81160524). We are indebted to the staff in the animal center and the Science &Technology Centre who provided assistance in the study. The authors would like to thank Dr. Ding-Feng Su, Prof. Zhang Wannian, Miss Wang jie, Miss Wang Shujing and Zhang Yan for their contributions to development and implementation of this study.
Conflict of interest
The authors declare that they have no competing interests.
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