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Protective effects of Lycium barbarum polysaccharide on neonatal rat primary cultured hippocampal neurons injured by oxygen-glucose deprivation and reperfusion

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Abstract

This study investigated the protective effects of Lycium barbarum polysaccharide (LBP) on alleviating injury from oxygen-glucose deprivation/reperfusion (OGD/RP) in primary cultured rat hippocampal neurons. Cultured hippocampal neurons were exposed to oxygen-glucose deprivation (OGD) for 2 h followed by a 24 h re-oxygenation. The MTT assay and the lactate dehydrogenase (LDH) release were used to determine the neuron viability. Superoxide dismutase (SOD), Glutathione peroxidase (GSH-PX), malondialdehyde (MDA) were determined by spectrophotometry using commercial kits. Mitochondrial membrane potential (MMP) and the intracellular free calcium concentration ([Ca2+]i) in hippocampal neurons were measured using the confocal laser scanning microscope (CLSM). Treatment with LBP (10–40 mg/l) significantly attenuated neuronal damage and inhibited LDH release in a dose-dependent manner. Furthermore, LBP enhanced activities of SOD and GSH-PX but it decreased their MDA content, inhibited [Ca2+]i elevation and decrease of MMP in ischemia–reperfusion treated hippocampal neurons. These findings suggested that LBP may be a potential neuroprotective agent for cerebral ischemia–reperfusion injury.

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Acknowledgments

The study was supported by the National Natural Science Foundation of China (Grant No. 30960506, 81160524). We are indebted to the staff in the animal center and the Science &Technology Centre who provided assistance in the study. The authors would like to thank Dr. Ding-Feng Su, Prof. Zhang Wannian, Miss Wang jie, Miss Wang Shujing and Zhang Yan for their contributions to development and implementation of this study.

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Pharmacology, Ningxia Medical University, Yinchuan, China

    Chen Rui, Hao Yinju, Zhu Qingluan, Wu Yang, Zhao Qipeng & Jiang Yuanxu

  2. College of Nursing, Ningxia Medical University, Yinchuan, China

    Li Yuxiang

  3. College of Pharmacy, Ningxia Medical University, Yinchuan, China

    Wang Hao

  4. Ningxia Key Lab of Craniocerbral Diseases of Ningxia Hui Autonomous Region, Yinchuan, China

    Ma Lin & Sun Tao

  5. School of Basis Medical Science, Ningxia Medical University, Yinchuan, China

    Liu Juan & Zhao Chengjun

  6. Key Laboratory of Fertility Preservation and Maintenance, Yinchuan, China

    Wang Yanrong

  7. Ningxia Medical University, Yinchuan, China

    Dai Xiuying

  8. Ningxia Hui Medicine Modern Engineering Research Center, Yinchuan, China

    Zhang Wannian

  9. Medical Sci-tech Research Center, Ningxia Medical University, Yinchuan, 750004, Ningxia, People’s Republic of China

    Yu Jianqiang

Authors
  1. Chen Rui
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  2. Li Yuxiang
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  3. Hao Yinju
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  4. Zhu Qingluan
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  7. Wang Hao
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  8. Ma Lin
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  9. Liu Juan
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  10. Zhao Chengjun
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  11. Jiang Yuanxu
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  12. Wang Yanrong
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  13. Dai Xiuying
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  14. Zhang Wannian
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  15. Sun Tao
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  16. Yu Jianqiang
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Corresponding author

Correspondence to Yu Jianqiang.

Additional information

Chen Rui and Li Yuxiang contributed equally to this work.

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Rui, C., Yuxiang, L., Yinju, H. et al. Protective effects of Lycium barbarum polysaccharide on neonatal rat primary cultured hippocampal neurons injured by oxygen-glucose deprivation and reperfusion. J Mol Hist 43, 535–542 (2012). https://doi.org/10.1007/s10735-012-9420-4

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  • DOI: https://doi.org/10.1007/s10735-012-9420-4

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