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Journal of Molecular Histology

, Volume 43, Issue 2, pp 137–143 | Cite as

High-frequency microsatellite instability and BRAF mutation (V600E) in unselected Serbian patients with colorectal cancer

  • Srdjan Markovic
  • Jadranka Antic
  • Neda Dragicevic
  • Richard Hamelin
  • Zoran Krivokapic
Article

Abstract

Microsatellite instability (MSI) is a genetic consequence of a MisMatch Repair defect in colorectal cancer (CRC). We compared clinicopathohistological features with MSI status of CRC and evaluated prognostic significance of MSI status and BRAF mutation in the group of MSI-H tumors. 155 primary CRCs were excised surgically, 2006–2008. MSI analysis was carried out using a fluorescence-based pentaplex polymerase chain reaction technique. BRAF mutation (V600E) was analyzed by direct sequencing in MSI-H tumors. For all patients were evaluated: age, gender, localization, tumor cell type, tumor differentiation, mucin production, lymphocytic infiltration (TILs) and TNM stage. Patients’ disease-free survival (DFS) was compared according to MSI and BRAF status using Kaplan–Meier test. Of the 155 CRCs, 19 (12.3%) were MSI-H, and 136 (87.7%) were MSS/L. BRAF mutations were found in 4 of the MSI-H tumors. Patients with MSI-H CRC had lower recurrence rate (log rank test; P = 0.04) than MSS/L group. Patients with MSI-H tumor and BRAF mutation had worse DFS than MSI-H tumors without this mutation (log rank test; P = 0.01). Most of the clinicopathologic characteristics of MSI-H CRC in Serbian patients are similar to those reported in previous studies. Patients with MSI tumor phenotype had favourable prognosis, but in those with BRAF mutation higher recurrence rate was observed.

Keywords

Microsatellite instability Colon cancer Clinicopathohistological features BRAF mutation 

Notes

Acknowledgments

This work was supported by Ministry of Science; Republic of Serbia: Grant No 41033.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Srdjan Markovic
    • 1
  • Jadranka Antic
    • 2
  • Neda Dragicevic
    • 2
  • Richard Hamelin
    • 3
    • 4
  • Zoran Krivokapic
    • 5
  1. 1.Center for Gastroenterology and Hepatology, Zvezdara University Clinical CenterBelgradeSerbia
  2. 2.Institute of Endocrinology, Diabetes and Metabolic Diseases, Clinical Center of SerbiaBelgradeSerbia
  3. 3.Université Pierre et Marie Curie-Paris6ParisFrance
  4. 4.INSERM, UMRS 938, Centre de Recherche Saint Antoine, Equipe “Instablité des Microsatellites et Cancers”ParisFrance
  5. 5.Center for Colorectal Surgery, Clinical Center of SerbiaBelgradeSerbia

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