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Journal of Molecular Histology

, Volume 43, Issue 1, pp 89–94 | Cite as

Cytogenetic instability of dental pulp stem cell lines

  • Monica Talarico Duailibi
  • Leslie Domenici Kulikowski
  • Silvio Eduardo Duailibi
  • Monica Vannucci Nunes Lipay
  • Maria Isabel Melaragno
  • Lydia Masako Ferreira
  • Joseph Phillip Vacanti
  • Pamela Crotty Yelick
Original Paper

Abstract

Human adult stem cells (hASCs) offer a potentially renewable source of cell types that are easily isolated and rapidly expanded for use in regenerative medicine and cell therapies without the complicating ethical problems that are associated with embryonic stem cells. However, the eventual therapeutic use of hASCs requires that these cells and their derivatives maintain their genomic stability. There is currently a lack of systematic studies that are aimed at characterising aberrant chromosomal changes in cultured ASCs over time. However, the presence of mosaicism and accumulation of karyotypic abnormalities within cultured cell subpopulations have been reported. To investigate cytogenetic integrity of cultured human dental stem cell (hDSC) lines, we analysed four expanded hDSC cultures using classical G banding and fluorescent in situ hybridisation (FISH) with X chromosome specific probe. Our preliminary results revealed that about 70% of the cells exhibited karyotypic abnormalities including polyploidy, aneuploidy and ring chromosomes. The heterogeneous spectrum of abnormalities indicates a high frequency of chromosomal mutations that continuously arise upon extended culture. These findings emphasise the need for the careful analysis of the cytogenetic stability of cultured hDSCs before they can be used in clinical therapies.

Keywords

Chromosomal abnormalities Cell transplantation Tooth tissue engineering Human dental stem cells 

Notes

Acknowledgments

We wish to thank the INCT- Biofabrication Institute, CNPq 573661/2008-1, FAPESP 08/57860-3, and the Rede Biofab, Ibero-American Network of Biofabrication—BIOFAB-CYTED (208RT0340). This work was supported by FAPESP grants 07- 58856-7, 07-51227-4, 07-59488-1, and NIH/NIDCR R01DE016132 (PCY), and R03TW007665 (PCY, MTD, SED) awards. We are also grateful to Dr. Waldyr Antonio Jorge, Ph.D., Oral Maxillofacial Surgery Course of Dental School, FFO–FOUSP Professor of University of São Paulo for contribution of the biological material access.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Monica Talarico Duailibi
    • 1
    • 2
  • Leslie Domenici Kulikowski
    • 4
    • 7
  • Silvio Eduardo Duailibi
    • 1
    • 2
    • 3
  • Monica Vannucci Nunes Lipay
    • 8
  • Maria Isabel Melaragno
    • 4
  • Lydia Masako Ferreira
    • 1
  • Joseph Phillip Vacanti
    • 5
  • Pamela Crotty Yelick
    • 6
  1. 1.Department of Plastic SurgeryFederal University of São Paulo, UNIFESP CTCMol, Center of Cellular and Molecular TherapySão PauloBrazil
  2. 2.National Institute of Science and Technology—Biofabrication Institute, INCT-BiofabrisSão PauloBrazil
  3. 3.Science and Technology Institute—Biomedical Engineering-UNIFESP-Sao José dos CamposSão PauloBrazil
  4. 4.Department of Morphology and GeneticsFederal University of São PauloSão PauloBrazil
  5. 5.Laboratory for Tissue Engineering and Organ Fabrication, Massachusetts General Hospital and Department of Surgery, and Harvard Medical SchoolBostonUSA
  6. 6.Department of Oral and Maxillofacial PathologyTufts UniversityBostonUSA
  7. 7.Department of Pathology, Cytogenomics LaboratoryUniversity of São Paulo, LIM 03São PauloBrazil
  8. 8.Division of EndocrinologyFederal University of São PauloSão PauloBrazil

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