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Developmental expression of plasma glutathione peroxidase during mouse organogenesis

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Abstract

Plasma glutathione peroxidase (pGPx) is an extracellular antioxidative selenoenzyme which has been detected in various adult tissues, but little is known about the expression and distribution of pGPx during embryogenesis. To investigate the expression patterns of pGPx during embryogenesis, we performed quantitative real-time PCR, in situ hybridization, Western blot, and immunohistochemistry analyses in whole embryos or each developing organ of mice on embryonic days (E)7.5–18.5. In whole embryos of E7.5–8.5, pGPx mRNA was more typically expressed in extra-embryonic tissues including ectoplacental cone, trophectoderm, and decidual cells than in embryos. However, after E9.5, pGPx mRNA and protein levels were increased in the embryos with differentiation and growth, but trended to gradually decrease in the extra-embryonic tissues until E18.5. In sectioned embryonic tissues on E13.5–18.5, pGPx mRNA and protein were mainly expressed in the developing nervous tissues, the sensory organs, and the epithelia of lung, skin, and intestine, the heart and artery, and the kidney. In particular, pGPx immunoreactivity was very strong in the developing liver. These results indicate that pGPx is spatio-temporally expressed in various embryonic organs as well as extra-embryonic tissues, suggesting that pGPx may function to protect the embryos against endogenous and exogenous reactive oxygen species during organogenesis.

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Acknowledgments

This work was supported by the Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0029709).

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Authors and Affiliations

  1. Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, 361-763, South Korea

    Ki Youn Jung, Jung-Min Yon, Mi-Ra Kim, Beom Jun Lee, Young Won Yun & Sang-Yoon Nam

  2. Laboratory of Mammalian Molecular Genetics, Department of Biochemistry, College of Science, Yonsei University, Seoul, 120-749, South Korea

    In-Jeoung Baek

  3. CKD Research Institute, Chong Kun Dang Pharm., P.O. Box 74, Chonan, 330-600, South Korea

    Se-Ra Lee

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  1. Ki Youn Jung
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  2. In-Jeoung Baek
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  3. Jung-Min Yon
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  5. Mi-Ra Kim
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Correspondence to Sang-Yoon Nam.

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Ki Youn Jung and In-Jeoung Baek were contributed equally to this article.

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Jung, K.Y., Baek, IJ., Yon, JM. et al. Developmental expression of plasma glutathione peroxidase during mouse organogenesis. J Mol Hist 42, 545–556 (2011). https://doi.org/10.1007/s10735-011-9362-2

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  • DOI: https://doi.org/10.1007/s10735-011-9362-2

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