Abstract
MT1-MMP and TIMP-2 are well known for their roles in remodelling of extracellular matrix components. However, reports are emerging on the involvement of these molecules in cell kinetics. In the rat incisor tooth, a shortening treatment increases the eruption and cell proliferation rates. However, the role of MT1-MMP and TIMP-2 proteins in these processes is still to be evaluated. Male Wistar rats were divided in two groups. In the normofunctional group (NF) the lower teeth of the rats remained in a normal eruption process. In the hypofunctional group (HP) rats their lower left incisor tooth was shortened every 2 days during 12 days. The eruption rate was estimated during the shortening period and MT1-MMP, TIMP-2 and Ki-67 protein expression from the odontogenic region was measured after the treatment. In HP groups an increase in eruption rate, and in MT1-MMP/TIMP-2 and Ki-67 expression were observed. We conclude that there is a relationship between the increase in eruption rate, and in levels of MT1-MMP, TIMP-2 and Ki-67 in the HP group. This suggests that MT1-MMP and TIMP-2 may have some role in cell proliferation during the eruption of the rat incisor tooth.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barbolina MV, Stack MS (2008) Membrane type 1-matrix metalloproteinase: substrate diversity in pericellular proteolysis. Semin Cell Dev Biol 19(1):24–33
Bar-Lev M, Michaeli Y, Zajicek G, Weinreb MM (1977) Role of attrition and occlusal contact in the physiology of the rat incisor: XI Kinetics of inner enamel epithelium during severely impeded eruption. J Dent Res 56(2):135–139
Bartlett JD, Simmer JP (1999) Proteinases in developing dental enamel. Crit Rev Oral Biol Med 10(4):425–441
Bartlett JD, Zhou Z, Skobe Z, Dobeck JM, Tryggvason K (2003) Delayed tooth eruption in membrane type-1 matrix metalloproteinase deficient mice. Connect Tissue Res 44(Suppl 1):300–304
Beertsen W, Holmbeck K, Niehof A, Bianco P, Chrysovergis K, Birkedal-Hansen H, Everts V (2002) On the role of MT1-MMP, a matrix metalloproteinase essential to collagen remodeling, in murine molar eruption and root growth. Eur J Oral Sci 110(6):445–451
Beertsen W, Holmbeck K, Niehof A, Bianco P, Chrysovergis K, Birkedal-Hansen H, Everts V (2003) Inhibition of molar eruption and root elongation in MT1-MMP-deficient mice. Connect Tissue Res 44(Suppl 1):298–299
Berkovitz BK, Thomas NR (1969) Unimpeded eruption in the root-resected lower incisor of the rat with a preliminary note on root transection. Arch Oral Biol 14(7):771–780
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Burn-Murdoch RA (1999) The length and eruption rates of incisor teeth in rats after one or more of them had been unimpeded. Eur J Orthod 21(1):49–56
Caron C, Xue J, Bartlett JD (1998) Expression and localization of membrane type 1 matrix metalloproteinase in tooth tissues. Matrix Biol 17(7):501–511
Casasco A, Calligaro A, Marchetti C, Poggi P, Brugnatelli S, Danova M, Fiocca R (1989) Immunocytochemical detection of proliferating cells in the rat tooth germ by monoclonal antibodies against 5-bromo-2’-deoxyuridine. Arch Oral Biol 34(1):65–69
Casasco A, Casasco M, Cornaglia AI, Mazzini G, De Renzis R, Tateo S (1995) Detection of bromo-deoxyuridine- and proliferating cell nuclear antigen-immunoreactivities in tooth germ. Connect Tissue Res 32(1–4):63–70
Chiba M, Tashiro T, Tsuruta M, Eto K (1976) Acceleration and circadian rhythm of eruption rates in the rat incisor. Arch Oral Biol 21(4):269–271
Chun YH, Yamakoshi Y, Yamakoshi F, Fukae M, Hu JC, Bartlett JD, Simmer JP (2010) Cleavage site specificity of MMP-20 for secretory-stage ameloblastin. J Dent Res 89(8):785–790
D’Alessio S, Ferrari G, Cinnante K et al (2008) Tissue inhibitor of metalloproteinases-2 binding to membrane-type 1 matrix metalloproteinase induces MAPK activation and cell growth by a non-proteolytic mechanism. J Biol Chem 283(1):87–99
Endo K, Takino T, Miyamori H, Kinsen H, Yoshizaki T, Furukawa M, Sato H (2003) Cleavage of syndecan-1 by membrane type matrix metalloproteinase-1 stimulates cell migration. J Biol Chem 278(42):40764–40770
Espírito Santo AR, Bartlett JD, Gibson CW, Li Y, Kulkarni AB, Line SR (2007) Amelogenin- and enamelysin (Mmp-20)-deficient mice display altered birefringence in the secretory-stage enamel organic extracellular matrix. Connect Tissue Res 48(1):39–45
Goldberg M, Septier D, Bourd K, Hall R, George A, Goldberg H, Menashi S (2003) Immunohistochemical localization of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the forming rat incisor. Connect Tissue Res 44(3–4):143–153
Harada H, Mitsuyasu T, Toyono T, Toyoshima K (2002) Epithelial stem cells in teeth. Odontology 90:1–6
Hayakawa T, Yamashita K, Ohuchi E, Shinagawa A (1994) Cell growth-promoting activity of tissue inhibitor of metalloproteinases-2 (TIMP-2). J Cell Sci 107(Pt 9):2373–2379
Hernandez-Barrantes S, Toth M, Bernardo MM et al (2000) Binding of active (57 kDa) membrane type 1-matrix metalloproteinase (MT1-MMP) to tissue inhibitor of metalloproteinase (TIMP)-2 regulates MT1-MMP processing and pro-MMP-2 activation. J Biol Chem 275(16):12080–12089
Holmbeck K, Bianco P, Caterina J et al (1999) MT1-MMP-deficient mice develop dwarfism, osteopenia, arthritis, and connective tissue disease due to inadequate collagen turnover. Cell 99(1):81–92
Lu Y, Papagerakis P, Yamakoshi Y, Hu JC, Bartlett JD, Simmer JP (2008) Functions of KLK4 and MMP-20 in dental enamel formation. Biol Chem 389(6):695–700
Nagano T, Kakegawa A, Yamakoshi Y, Tsuchiya S, Hu JC, Gomi K, Arai T, Bartlett JD, Simmer JP (2009) Mmp-20 and Klk4 cleavage site preferences for amelogenin sequences. J Dent Res 88(9):823–828
Ness AR (1965) Eruption rates of impeded and unimpeded mandibular incisors of the adult laboratory mouse. Arch Oral Biol 10(3):439–451
Neves JS, Salmon CR, Omar NF, Narvaes EA, Gomes JR, Novaes PD (2009) Immunolocalization of CSF-1, RANKL and OPG in the enamel-related periodontium of the rat incisor and their implications for alveolar bone remodeling. Arch Oral Biol 54(7):651–657
Novaes PD, Barbosa SC, Omar NF, Neves JS, Narvaes EAO, Soares MAM, Gomes JR (2009) Ultrastructural Aspects of Frequency of Ciliated Fibroblasts in the Periodontal Ligament of Incisor Tooth of Rats Submitted to Different Eruption Conditions. Animal Biol J 1(2):59–69
Ohshima H, Nakasone N, Hashimotoa E, Sakaia H, Nakakura OK, Harada H (2005) The eternal tooth germ is formed at the apical end of continuously growing teeth. Arch Oral Biol 50:153–157
Prasad M, Butler WT, Qin C (2010) Dentin sialophosphoprotein in biomineralization. Connect Tissue Res. Apr2. Epub ahead of print
Scholzen T, Gerdes J (2000) The Ki-67 protein: from the known and the unknown. J Cell Phys 182:311–322
Schwab W, Harada H, Goetz W, Nowicki M, Witt M, Kasper M, Barth K (2007) Immunocytochemical and biochemical detection of EMMPRIN in the rat tooth germ: differentiation-dependent co-expression with MMPs and co-localization with caveolin-1 in membrane rafts of dental epithelial cells Histochem. Cell Biol 128(3):195–203
Sternlicht MD, Werb Z (2001) How matrix metalloproteinases regulate cell behavior. Annu Rev Cell Dev Biol 17:463–516
Stetler-Stevenson WG (2008) Tissue inhibitors of metalloproteinases in cell signaling: metalloproteinase-independent biological activities. Sci Signal 8; 1(27):re6
Stetler-Stevenson WG, Bersch N, Golde DW (1992) Tissue inhibitor of metalloproteinase-2 (TIMP-2) has erythroid-potentiating activity. FEBS Lett 20; 296 (2):231–234
Sun Z, Carpiaux W, Fan D, Fan Y, Lakshminarayanan R, Moradian-Oldak J (2010) Apatite reduces amelogenin proteolysis by MMP-20 and KLK4 in vitro. J Dent Res 89(4):344–348
Wick M, Burger C, Brusselbach S, Lucibello FC, Muller R (1994) A novel member of human tissue inhibitor of metalloproteinases (TIMP) gene family is regulated during G1 progression, mitogenic stimulation, differentiation and senescence. J Biol Chem 269:18953–18960
Xie M, Jiao T, Chen Y, Xu C, Li J, Jiang X, Zhang F (2010) EMMPRIN (basigin/CD147) is involved in the morphogenesis of tooth germ in mouse molars. Histochem Cell Biol 133(5):585–594
Yamakoshi Y (2009) Dentinogenesis and Dentin Sialophosphoprotein (DSPP). J Oral Biosci 10; 51(3):134
Yamakoshi Y, Hu JC, Iwata T, Kobayashi K, Fukae M, Simmer JP (2006) Dentin ialophosphoprotein is processed by MMP-2 and MMP-20 in vitro and in vivo. Biol Chem 281(50):38235–38243
Yoshiba N, Yoshiba K, Stoetzel C, Perrin-Schmitt F, Cam Y, Ruch JV, Hosoya A, Ozawa H, Lesot H (2003) Temporospatial gene expression and protein localization of matrix metalloproteinases and their inhibitors during mouse molar tooth development. Dev Dyn 228:105–112
Yoshiba N, Yoshiba K, Stoetzel C, Perrin-Schmitt F, Cam Y, Ruch JV, Hosoya A, Ozawa H, Lesot H (2006) Differential regulation of TIMP-1, -2, and -3 mRNA and protein expressions during mouse incisor development. Cell Tissue Res 324(1):97–104
Zajicek G (1976) The rodent incisor tooth proliferon. Cell Tissue Kinet 9(3):207–214
Zajicek G, Michaeli Y, Weinreb MM (1972) Kinetics of the inner enamel epithelium in the adult rat incisor during accelerated eruption. Cell Tissue Kinet 5:35–39
Acknowledgments
Our thanks for FAPESP—SÃO PAULO, Brazil, by the financial support of this research as well as to Dr. Ann J. Stocker from Centre for Environmental Stress and Adaptation Research (CESAR), Department of Genetics, University of Melbourne, Australia, by your kindly in prepare the English revision of this manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gomes, J.R., Omar, N.F., dos Santos Neves, J. et al. Increase of MT1-MMP, TIMP-2 and Ki-67 proteins in the odontogenic region of the rat incisor post-shortening procedure. J Mol Hist 41, 333–341 (2010). https://doi.org/10.1007/s10735-010-9295-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10735-010-9295-1