Journal of Molecular Histology

, Volume 41, Issue 4–5, pp 193–197 | Cite as

Immunohistochemical analysis of ageing and osteoarthritic articular cartilage

  • Neeru Goyal
  • Madhur Gupta
  • Kusum Joshi
  • Onkar Nath Nagi
Original Paper


Articular cartilage degeneration seen in osteoarthritis is primarily the consequence of events within the articular cartilage that leads to the production of proteases by chondrocytes. 22 osteoarthritic cartilage specimens were obtained from patients with primary osteoarthritis (46–81 years) undergoing total knee replacement. 12 age-matched (41–86 years) and 16 young (16–40 years) non-osteoarthritic control cartilage specimens were obtained from the cadavers in the department of Anatomy and from patients undergoing lower limb amputation in Trauma center of PGIMER, Chandigarh. 5 μ thick paraffin sections were stained for osteocalcin, osteopontin, osteonectin and alkaline phosphatase to analyze their expression in hypertrophied chondrocytes and osteoarthritic cartilage matrix and to compare the staining intensity with that of normal ageing articular cartilage. Immunohistochemical staining of tissue sections revealed moderate to strong cytoplasmic staining for all four stains in all the specimens of the osteoarthritic group compared to age-matched control. The immunohistochemical scores were significantly higher in the osteoarthritic group for all four stains. The features of the osteoarthritic articular cartilage were markedly different from the non-osteoarthritic age-matched articular cartilage suggesting that osteoarthritis is not an inevitable feature of aging.


Cartilage Collagen Osteoarthritis Osteocalcin Osteonectin Osteopontin 



The authors wish to thank the Indian Council of Medical Research, New Delhi for providing financial assistance for this research work.


  1. Balcerzak M, Hamade E, Zhang L, Pikula S, Azzar G, Radisson J, Bandorowicz-Pikula J, Buchet R (2003) The roles of annexins and alkaline phosphatase in mineralization process. Acta Biochim Pol 50:1019–1038PubMedGoogle Scholar
  2. Brambilla E, Negoescu A, Gazzeri S, Lantuejoul S, Moro D, Brambilla C, Coll JC (1996) Apoptosis related factors p53, Bcl-2, Bax in neuroendocrine Luna tumors. Am J Pathol 149:1941–1952PubMedGoogle Scholar
  3. Goepfert C, Lutz V, Lünse S, Kittel S, Wiegandt K, Kammal M, Püschel K, Pörtner R (2010) Evaluation of cartilage specific matrix synthesis of human articular chondrocytes after extended propagation on microcarriers by image analysis. Int J Artif Organs 33(4):204–219PubMedGoogle Scholar
  4. Goldring MB, Goldring SR (2010) Articular cartilage and subchondral bone in the pathogenesis of osteoarthritis. Ann N Y Acad Sci 1192:230–237CrossRefPubMedGoogle Scholar
  5. Goyal N, Gupta M, Joshi K, Nagi ON (2006) Osteoarthritic femoral articular cartilage of knee joint in man. Nepal Med Coll J 8:88–92PubMedGoogle Scholar
  6. Hughes LC, Archer CW, Gwynn I (2005) The ultrastructure of mouse articular cartilage: collagen orientation and implications for tissue functionality. A polarized light and scanning electron microscope study and review. Eur Cell Mater 9:68–84PubMedGoogle Scholar
  7. Kamihagi K, Katayama M, Ouchi R, Kato I (1994) Osteonectin/SPARC regulates cellular secretion rates of fibronectin and laminin extracellular matrix proteins. Biochem Biophys Res Commun 200:423–428CrossRefPubMedGoogle Scholar
  8. Kirsch T (2006) Determinants of pathological mineralization. Curr Opin Rheumatol 18(2):174–180CrossRefPubMedGoogle Scholar
  9. Lian JB, McKee MD, Todd AM, Gerstenfeld LC (1993) Induction of bone-related proteins, osteocalcin and osteopontin and their matrix ultrastructural localization with development of chondrocyte hypertrophy in vitro. J Cell Biochem 52:206–219CrossRefPubMedGoogle Scholar
  10. Nakamura S, Kamihagi K, Satkeda H, Katayama M, Pan H, Okamota H, Noshira M, Takahashi K, Yoshira Y, Shimmei M, Okada Y, Kato Y (1996) Enhancement of SPARC (Osteonectin) synthesis in arthritic cartilage. Arthritis Rheum 39:539–551CrossRefPubMedGoogle Scholar
  11. Nakase T, Takaoka K, Hirakawa K, Hirota S, Takemura T, Onoue H, Takebayashi K, Kitamura Y, Nomura S (1994) Alterations in the expression of osteonectin, osteopontin and osteocalcin mRNAs during the development of skeletal tissues in vivo. Bone Miner 26:109–122CrossRefPubMedGoogle Scholar
  12. Pullig O, Weseloh G, Gauer S, Swoboda B (2000a) Osteopontin is expressed by adult human osteoarthritic chondrocytes: protein and mRNA analysis of normal and osteoarthritic cartilage. Matrix Biol 19:245–255CrossRefPubMedGoogle Scholar
  13. Pullig O, Weseloh G, Ronneberger DL, Kakonen SM, Swoboda B (2000b) Chondrocyte differentiation in human osteoarthritis: expression of Osteocalcin in normal and osteoarthritic cartilage and bone. Calcify Tissue Int 67:230–240CrossRefGoogle Scholar
  14. Rosenthal AK, Gohr CM, Uzuki M, Masuda I (2007) Osteopontin promotes pathologic mineralization in articular cartilage. Matrix Biol 26:96–105CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Neeru Goyal
    • 1
  • Madhur Gupta
    • 2
  • Kusum Joshi
    • 3
  • Onkar Nath Nagi
    • 4
  1. 1.Department of AnatomyChristian Medical CollegeLudhianaIndia
  2. 2.Department of AnatomyGian Sagar Medical CollegeBanurIndia
  3. 3.Department of HistopathologyPostgraduate Institute of Medical Education and ResearchChandigarhIndia
  4. 4.Department of OrthopaedicsShri Ganga Ram HospitalNew DelhiIndia

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