Journal of Molecular Histology

, Volume 41, Issue 1, pp 19–25 | Cite as

The role of matrix metalloproteinases 2 and 9 during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide

  • Ana Carolina Cuzzuol Fracalossi
  • Sandra Regina Miranda
  • Celina Tijuko Fujiyama Oshima
  • Marcello Franco
  • Daniel Araki Ribeiro
Original Paper


Matrix metalloproteinases (MMPs) are implicated in a wide range of physiological and pathological processes, including morphogenesis, wound healing, angiogenesis, inflammation, and cancer. The purpose of this study was to characterize the role of MMPs as depicted by the expression of MMP-2 and MMP-9 during 4-nitroquinoline 1-oxide-induced rat tongue carcinogenesis. Male Wistar rats were distributed into three groups of 10 animals each and treated with 4-nitroquinoline 1-oxide solution at 50 ppm through their drinking water for 4, 12, and 20 weeks. Ten animals were used as control group. No histopathological abnormalities were induced in the epithelium after 4 weeks of carcinogen exposure; however, immunoexpression of MMP-2 was noticed. The same picture occurred to MMP-9, in which positive expression was detected for this immunomarker. MMP-2 and MMP-9 showed positive expression either in pre-neoplastic lesions at 12 weeks following carcinogen exposure or in well-differentiated squamous cell carcinoma induced after 20 weeks of treatment with 4NQO. Taken together, our results support the belief that MMP-2 and MMP-9 play important role during malignant transformation and conversion of oral mucosa as assessed by immunohistochemistry.


Oral squamous cell carcinoma 4-nitroquinoline 1-oxide Immunohistochemistry Matrix metalloproteinase 



This work was supported by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) (Grant numbers: 07/01228-4 and 07/08210-3), CNPq and CAPES. DAR is a recipient of the CNPq fellowship.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Ana Carolina Cuzzuol Fracalossi
    • 1
  • Sandra Regina Miranda
    • 1
  • Celina Tijuko Fujiyama Oshima
    • 1
  • Marcello Franco
    • 1
  • Daniel Araki Ribeiro
    • 1
    • 2
  1. 1.Department of PathologyFederal University of Sao Paulo, UNIFESPSao PauloBrazil
  2. 2.Department of BiosciencesFederal University of Sao Paulo, UNIFESPSantosBrazil

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