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Imbalance of RANK, RANKL and OPG expression during tibial fracture repair in diabetic rats

  • Fernanda Penna Lima Guedes de Amorim
  • Sócrates Souza Ornelas
  • Sandoval Felicíssimo Diniz
  • Aline Carvalho Batista
  • Tarcília Aparecida da Silva
Original Paper

Abstract

To clarify the mechanisms of altered bone repair in the diabetic state, we investigated RANK, RANKL and OPG expression by immunohistochemistry and RT-PCR in the fracture sites of rats that were either healthy or made diabetic by alloxan. Histomorphometric analysis of the fracture site at 7 days after fracture revealed that diabetic rats (db) have significantly less hard tissue formation at the fracture site, compared to controls. The number of RANK, RANKL and OPG positive cells was decreased in the db group; however, the RANKL/OPG ratio was similar in controls and db at this time. At day 14, numbers of RANKL and OPG positive cells and the mRNA expression for these markers were higher in the control group than in db (P = 0.008). The RANKL/OPG ratio in the db group was greater than in controls. Our results demonstrate an imbalance of RANKL/OPG expression associated with diabetes that may contribute to the delay of fracture repair during the course of diabetes.

Keywords

RANK RANKL OPG Diabetes Facture repair 

Notes

Acknowledgments

The authors are grateful to Gabriela Mariângela Farias de Oliveira, Édelyn Cristina Nunes Silva and Renata Ribeiro de Souza for their helpful technical assistance. This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Fernanda Penna Lima Guedes de Amorim
    • 1
  • Sócrates Souza Ornelas
    • 2
  • Sandoval Felicíssimo Diniz
    • 1
  • Aline Carvalho Batista
    • 3
  • Tarcília Aparecida da Silva
    • 4
    • 5
  1. 1.Department of Pathology, Faculty of MedicineUniversity of BrasíliaBrasilia, Distrito FederalBrazil
  2. 2.Department of Molecular Pathology, Faculty of MedicineUniversity of BrasíliaBrasilia, Distrito FederalBrazil
  3. 3.Department of Stomatology (Oral Pathology), Dental SchoolUniversidade Federal de GoiásGoianiaBrazil
  4. 4.Department of Oral Pathology and Surgery, Faculty of DentistryUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  5. 5.Departamento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de OdontologiaUniversidade Federal de Minas GeraisBelo HorizonteBrazil

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