Journal of Molecular Histology

, Volume 38, Issue 4, pp 373–376 | Cite as

YKL-40 protein expression in normal adult human tissues – an immunohistochemical study

  • Merete Ringsholt
  • Estrid V. S. Høgdall
  • Julia S. Johansen
  • Paul A. Price
  • Lise H. Christensen

Erratum to: J Mol Hist DOI 10.1007/s10735-006-9075-0

Due to an unfortunate mistake, Figs. 1–6 were printed in black and white.
Fig. 1

(AD) Parathyroid tissue stained for YKL-40 with the primary specific antibody (mAb 201F9) (A) and for a positive control antibody (mAb 115F6D10) (B). Both antibodies give an intense cytoplasmic staining of the endocrine chief cells. An island of fibrous tissue in the centre containing just a few stained chief cells remains unstained (×40) C, D. Appendix mucosa incubated with (C) and without (D) preabsorption of the mAb 201F9 antibody. No staining is seen when the mAB 201F9 was preabsorbed with the YKL-40 protein (C) (×40)

Fig. 2

(A) Fibers of a peripheral nerve. The cells (arrows) are intensely stained (×40). (B) Brain tissue, gray matter. The neurons are intensely stained (black arrows). The microglia are less intensely stained or not stained at all (white arrows). The dark colour of the background is due to the entanglement of YKL-40 positive neurofibrils and dendrites, which are normally demonstrated by silver stain (×40). (C) Placental tissue. Both trophoblast (arrow) and the endothelium of capillaries (arrowheads) are intensely stained (×40). (D) An area from the appendix wall containing both stained (black arrows) and unstained (white arrows) polymorph nuclear granulocytes. Some stained debris is seen in the background to the left. Two vessels with positive endothelium and negative muscle walls (m) are seen in the centre (×40)

Fig. 3

(A) Parasympathetic ganglion of the myenteric plexus of the colon. Individual cells are intensely stained (arrow) (×40). (B) Pancreas section with intensely stained islet cells (arrow) and more weakly stained acinar and ductal cells (×40). (C) Thyroid tissue. The follicular epithelial cells are weakly to moderately stained (arrows) (×40). (D) Liver tissue with a portal tract centrally. The hepatocytes are moderately stained (white arrow), whereas the bile duct epithelial cells are intensely stained (black arrow) (×20)

Fig. 4

(A) Parotic gland. The ducts are intensely stained (arrows) in contrast to the weakly stained serous alveolar cells (×40). (B) Breast lobule. The secretory tubules are moderately to intensely stained (arrows). The ducts are negative or weakly positive (arrowhead) (×40). (C) Corpus endometrium of the uterus in proliferation phase. The proliferating glands are moderately to intensely stained (arrows) (×40). (D) Kidney parenchyme. Some of the tubules are moderately to intensely stained (arrows). The glomerulus is negative (arrowhead). Endothelial cells of a muscular artery (top left) are positive, whereas the muscle cells of the wall (m) are negative (×40)

Fig. 5

(A) Squamous epithelium of palatine tonsil and underlying lymphatic tissue (lt). The intermediate and lower parts of the epithelium are stained (arrows). The superficial layers are negative (n) (×40). (B) Respiratory epithelium of bronchus. Some cells are intensely stained (arrow), others are weakly to moderately positive (×40). (C) Transformation zone of the cervical canal/portio. The mucous glands of the cervix are negative (arrows). The emerging squamous epithelium is moderately positive (arrowhead) (×40). (D) Gastric mucosa. The parietal cells are intensely stained (black arrows). The chief cells and the surface mucous epithelial cells are more weakly stained (white arrows) (×40)

Fig. 6

(A) Synovial lining cells showing a weak to moderate staining (black arrows). Scattered plasma cells are stained as well (white arrows) (×40). (B) Cartilage abutting compact bone tissue (upper left). The chondrocytes are weakly to moderately stained (arrows) (×40). (C) Trabecula of bone tissue separated by fibrous tissue. Both osteoblasts and osteocytes (arrows) are moderately stained (×40). (D) Bone marrow section. Most hematogenous precursors, including megacaryocytes, are positive (arrows). Only some lymphocytes (l) and all the erythrocytes (e) are negative (×40)

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Merete Ringsholt
    • 1
    • 2
  • Estrid V. S. Høgdall
    • 1
    • 3
  • Julia S. Johansen
    • 4
  • Paul A. Price
    • 5
  • Lise H. Christensen
    • 1
  1. 1.Department of PathologyBispebjerg Hospital, University of CopenhagenCopenhagenDenmark
  2. 2.Faculty of Medical Laboratory ScienceUniversity College OeresundCopenhagen NDenmark
  3. 3.Department of Virus, Hormones and CancerDanish Cancer SocietyCopenhagenDenmark
  4. 4.Department of RheumatologyHerlev Hospital, University of CopenhagenCopenhagenDenmark
  5. 5.Department of BiologyUniversity of California, San DiegoLa Jolla, San DiegoUSA

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