Journal of Molecular Histology

, Volume 38, Issue 1, pp 45–52 | Cite as

Expression profiling of liver receptor homologue 1 (LRH-1) in mouse tissues using tissue microarray

  • Hiroyuki Higashiyama
  • Mine Kinoshita
  • Satoshi Asano
Original Paper


Liver receptor homologue 1 (LRH-1) is a nuclear receptor that plays important roles in lipid homeostasis and embryogenesis. To elucidate systemic physiological functions of LRH-1, we used tissue microarray-based immunohistochemistry to examine the tissue distribution and localization of LRH-1 in adult mouse tissues. LRH-1 immunoreactivity was observed in the nucleus of multiple epithelial lineage cells in the digestive system (including absorptive epithelial cells in the small and large intestines, goblet cells, acinar cells of the exocrine glands, chief cells and mucus neck cells in the stomach, granular and prickle layer cells in the tongue and forestomach, and gall bladder epithelium); respiratory system (alveolar type II cells); and urinary system (transitional epithelium). Nuclear LRH-1 immunoreactivity was also localized in cells involved in fatty acid/glucose metabolism, including hepatocytes, brown adipocytes, and cardiomyocytes, and neurons involved in the regulation of food intake, including the arcuate nucleus in the hypothalamus and paraventricular nucleus of thalamus. Additionally, LRH-1 immunoreactivity was observed in testicular Leydig cells and ovarian follicular cells. These data suggest that LRH-1 functions in multiple organ systems to regulate epithelial cell physiology and differentiation, energy metabolism, and reproduction.


LRH-1 Tissue microarray Immunohistochemistry Localization Nuclear receptor 



We thank Dr. Bryan Goodwin and Michael A. Luther for reviewing this manuscript.

Supplementary material

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ESM 1 (XLS 26 kb)
10735_2007_9077_MOESM2_ESM.ppt (874 kb)
ESM 2 (PPT 874 kb)


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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Hiroyuki Higashiyama
    • 1
  • Mine Kinoshita
    • 1
  • Satoshi Asano
    • 1
  1. 1.Pharmacology DepartmentTsukuba Research LaboratoriesTsukubaJapan

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