Journal of Molecular Histology

, Volume 35, Issue 6, pp 581–587 | Cite as

Laser-assisted microdissection of the kidney: Fundamentals and applications

  • Gregory L. Blakey
  • Zoltan G. Laszik


Laser-assisted microdissection (LAM) permits the procurement of relatively pure cell populations from histological sections. When applied to the kidney, LAM combined with molecular biological techniques has expanded our understanding of renal biology and pathology. Both frozen and fixed renal tissues can be microdissected. However, sample type and tissue processing can influence the quality of molecular data generated. Data analysis may also be complicated by relative variations in gene expression levels. Importantly, preliminary studies have shown that molecular data obtained following LAM on the kidney can offer new diagnostic and prognostic information. Thus, LAM and molecular markers may eventually become incorporated into the routine kidney biopsy examination.


Gene Expression Data Analysis Cell Population Molecular Marker Gene Expression Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Baboolal K, Jones GA, Janezic A, Griths DR, Jurewicz WA (2002)Molecular and structural consequences of early renal allo-graft injury.Kidney Int 61:686-696.CrossRefPubMedGoogle Scholar
  2. Banks RE, Dunn MJ, Forbes MA, Stanley A, Pappin D, Naven T, Gough M, Harnden P, Selby PJ (1999)The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis-preliminary ndings.Electrophoresis 20:689-700.CrossRefPubMedGoogle Scholar
  3. Cohen CD, Gro¨ne HJ, Gro¨ne EF, Nelson PJ, Schlo¨ndor.D, Kret-zler M (2002)Laser microdissection and gene expression analysis on formaldehyde-xed archival tissue.Kidney Int 61:125-132.CrossRefGoogle Scholar
  4. Craven RA, Totty N, Harnden P, Selby PJ, Banks RE (2002)Laser capture microdissection and two-dimensional polyacrylamide gel electrophoresis:Evaluation of tissue preparation and sample limi-tations.Am J Pathol 160:815-822.PubMedGoogle Scholar
  5. Dimmler A, Haas CS, Cho S, Hattler M, Forster C, Peters H, Scho¨cklmann HO, Amann K (2003)Laser capture microdissec-tion and real-time PCR for analysis of glomerular endothelin-1 gene expression in mesangiolysis of rat anti-thy 1.1 and murine habu snake venom glomerulonephritis.Diagn Mol Pathol 12: 108-117.CrossRefPubMedGoogle Scholar
  6. Eikmans M, Baelde HJ, Hagen EC, Paul LC, Eilers PH, de Heer E, Bruijn JA (2003)Renal mRNA levels as prognostic tools in kidney diseases.J Am Soc Nephrol 14:899-907.CrossRefPubMedGoogle Scholar
  7. Eikmans M, Sijpkens YW, Baelde HJ, de Heer E, Paul LC, Bruijn JA (2002)High transforming growth factor-beta and extracellular matrix mRNA response in renal allografts during early acute rejec-tion is associated with absence of chronic rejection.Transplanta-tion 73:573-579.CrossRefGoogle Scholar
  8. Fries JWU, Pakula A, Roth T, Dienes H-P, Odenthal M (2002) Quantitation of in.ammatory and proliferative genes as disease markers in laser-microdissected formalin-xed and para.nized glomeruli from human renal biopsies.Gene Funct Dis 3:98-108.CrossRefGoogle Scholar
  9. Inoue K, Sakurada Y, Murakami M, Shirota M, Shirota K (2003) Detection of gene expression of vascular endothelial growth factor and.k-1 in the renal glomeruli of the normal rat kidney using the laser microdissection system.Virchows Arch 442:159-162.PubMedGoogle Scholar
  10. Karbowniczek M, Yu J, Henske EP (2003)Renal angiomyolipomas from patients with sporadic lymphangiomyomatosis contain both neoplastic and non-neoplastic vascular structures.Am J Pathol 162:491-500.PubMedGoogle Scholar
  11. Kim D, Garrett SH, Sens MA, Somji S, Sens DA (2002)Metallothi-onein isoform 3 and proximal tubule vectorial active transport. Kidney Int 61:464-472.CrossRefPubMedGoogle Scholar
  12. Kohda Y, Murakami H, Moe OW, Star RA (2000)Analysis of seg-mental renal gene expression by laser capture microdissection. Kidney Int 57:321-331.CrossRefPubMedGoogle Scholar
  13. Mitterbauer C, Schwarz C, Hauser P, Steininger R, Regele HM, Rosenkranz A, Oberbauer R (2003)Impaired tubulointerstitial expression of endothelin-1 and nitric oxide isoforms in donor kid-ney biopsies with postischemic acute renal failure.Transplantation 76:715-720.CrossRefPubMedGoogle Scholar
  14. Murakami H, Liotta L, Star RA (2000)IF-LCM:Laser capture microdissection of immuno.uorescently de ned cells for mRNA analysis.Kidney Int 58:1346-1353.CrossRefPubMedGoogle Scholar
  15. Nagasawa Y, Takenaka M, Matsuoka Y, Imai E, Hori M (2000) Quantitation of mRNA expression in glomeruli using laser-manip-ulated microdissection and laser pressure catapulting.Kidney Int 57:717-723.PubMedGoogle Scholar
  16. Nakajima H, Takenaka M, Kaimori J-Y, Nagasawa Y, Kosugi A, Kawamoto S, Imai E, Hori M, Okubo K (2002)Gene expression pro le of renal proximal tubules regulated by proteinuria.Kidney Int 61:1577-1587.CrossRefPubMedGoogle Scholar
  17. Schmid H, Cohen CD, Henger A, Irrgang S, Schlo¨ndor.D, Kretzler M (2003a,b)Validation of endogenous controls for gene expres-sion analysis in microdissected human renal biopsies.Kidney Int 64:356-360.CrossRefPubMedGoogle Scholar
  18. Schmid H, Henger A, Cohen CD, Frach K, Gro¨ne HJ, Schlo¨ndor. D, Kretzler M (2003a, b)Gene expression pro les of podocyte-associated molecules as diagnostic markers in acquired proteinuric diseases.J Am Soc Nephrol 14:2958-2966.CrossRefPubMedGoogle Scholar
  19. Selvey S, Thompson EW, Matthaei K, Lea RA, Irving MG, Griffths LR (2001)b actin-an unsuitable internal control for RT-PCR. Mol Cell Probes 15:307-311.CrossRefPubMedGoogle Scholar
  20. Silva FG, Nadasdy T, Laszik Z (1993)Immunohistochemical and lectin dissection of the human nephron in health and disease.Arch Pathol Lab Med 117:1233-1239.PubMedGoogle Scholar
  21. Striker LJ, Striker GE (2003)Windows on renal biopsy interpreta-tion:Does mRNA analysis represent a new gold standard?JAm Soc Nephrol 14:1096-1098.CrossRefGoogle Scholar
  22. Tanji N, Ross MD, Cara A, Markowitz GS, Klotman PE, D'Agati VD (2001)Effect of tissue processing on the ability to recover nucleic acid from speci c renal tissue compartments by laser capture microdissection.Exp Nephrol 9:229-234.CrossRefPubMedGoogle Scholar
  23. Thellin O, Zorzi W, Lakaye B, de Borman B, Coumans B, Hennen G, Grisar T, Igout A, Heinen E (1999)Housekeeping genes as internal standards:Use and limits.J Biotechnol 75:291-295.CrossRefPubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.Department of PathologyUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA

Personalised recommendations