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Journal of Molecular Histology

, Volume 35, Issue 6, pp 639–646 | Cite as

Expression of prohibitin 3′ untranslated region suppressor RNA alters morphology and inhibits motility of breast cancer cells

  • Sharmila Manjeshwar
  • Megan R. Lerner
  • Xiao-Ping Zang
  • Dannielle E. Branam
  • J. Thomas Pento
  • Mary M. Lane
  • Stan A. Lightfoot
  • Daniel J. Brackett
  • Eldon R. Jupe
Article

Abstract

The prohibitin 3′ untranslated region (3′UTR) belongs to a novel class of non-coding regulatory RNAs. It arrests cell cycle progression by blocking G1-S transition in breast and other cancers. Our previous studies comparing MCF7 derived clones constitutively expressing a common allelic form of prohibitin RNA (UTR/C) to various controls demonstrated that it functions as a tumor suppressor. Here, we further characterized the morphology and motility of these transgenic breast cancer cells when grown in cell culture and on nude mice. In contrast to empty vector (EV) cells, UTR/C cells were observed to grow in an organized manner with more cell-cell contact and differentiate into structures with a duct-like appearance. Computer assisted cytometry to evaluate differences in nuclear morphology was performed on UTR/C and EV tissues from nude mice. Receiver operator curve areas generated using a logistic regression model were 0.8, indicating the ability to quantitatively distinguish UTR/C from EV tissues. Keratinocyte growth factor-induced motility experiments showed that migration of UTR/C cells was significantly reduced (80–90%) compared to EV cells. Together, these data indicate that this novel 3′UTR influences not only the tumorigenic phenotype but also may play a role in differentiation and migration of breast cancer cells.

Keywords

Breast Cancer Cell Nude Mouse Untranslated Region Empty Vector Operator Curve 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Sharmila Manjeshwar
    • 1
  • Megan R. Lerner
    • 2
  • Xiao-Ping Zang
    • 3
  • Dannielle E. Branam
    • 4
  • J. Thomas Pento
    • 3
  • Mary M. Lane
    • 2
  • Stan A. Lightfoot
    • 5
  • Daniel J. Brackett
    • 2
  • Eldon R. Jupe
    • 6
  1. 1.InterGenetics, Inc. and Program in Immunobiology and CancerOklahoma Medical Research FoundationOklahoma CityUSA
  2. 2.Department of SurgeryUniversity of Oklahoma Health Sciences Center and Veterans Affairs Medical CenterOklahoma CityUSA
  3. 3.Department of Pharmaceutical Sciences, College of PharmacyUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA
  4. 4.Program in Immunobiology and CancerOklahoma Medical Research FoundationOklahoma CityUSA
  5. 5.Department of PathologyUniversity of Oklahoma Health Sciences Center and Veterans Affairs Medical CenterOklahoma CityUSA
  6. 6.InterGenetics, Inc.; Program in Immunobiology and CancerOklahoma Medical Research FoundationUSA

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