Plant Growth Regulation

, Volume 85, Issue 1, pp 133–142 | Cite as

Rice gene, BBH/Lsi1, regulates the color of rice hull by reducing the absorption and deposition of silicon and accumulating excess flavonoid

  • Chengcong Yang
  • Dongdong Zeng
  • Ran Qin
  • Md. Alamin
  • Xiaoli Jin
  • Chunhai Shi
Original paper


The color of grain hull is one of the remarkable traits which can be easily identified after heading, especially at the mature stage. In present study, a discoloration hull mutant named black–brown hull (bbh), was obtained from an indica cultivar Zhenong 34 by mutagenesis with ethyl methane sulfonate (EMS). The hull color of bbh mutant initially appeared black–brown on the 15th day and became completely black–brown on the 30th day after heading. The root of bbh was shorter than that of WT during the whole growth period. Furthermore, the total flavonoids content in the hulls and the silicon deposition in the leaves of bbh were both remarkably higher than WT. The results of map-based cloning indicated that the mutant trait of bbh was controlled by a single recessive nuclear gene, which was located on the long arm of chromosome 2 with a physical distance of 64.9 kb. The comparison of gDNA sequence between bbh and WT showed that there was a substitution from T to C (+ 2360) in the third exon of LOC_Os02g51110. BBH was an allelic gene of Lsi1, which encoded a water channel protein (aquaporin) involving in silicon uptake. The expression levels of the genes related to silicon absorption, transportation and distribution were detected to be up- or down-regulated at different stages in bbh root. Moreover, the transcripts of structural enzymatic genes involved in flavonoid biosynthesis in hulls also had apparent differences between WT and bbh mutant. Above all, the results revealed that BBH/Lsi1 was responsible to the black–brown hull by reducing the absorption and deposition of silicon and accumulating overmuch flavonoid.


Rice (Oryza sativa L.) Black–brown hull Silicon Flavonoid Gene mapping 



This work was supported by the Science and Technology Office of Zhejiang Province (2012C12901-2, 2016C32085 and 2016C02050-6) and the National Key Research and Development of China (2017YFD0100300-5).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10725_2018_379_MOESM1_ESM.docx (16.3 mb)
Supplementary material 1 (DOCX 16727 KB)


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Chengcong Yang
    • 1
  • Dongdong Zeng
    • 1
  • Ran Qin
    • 1
  • Md. Alamin
    • 1
  • Xiaoli Jin
    • 1
  • Chunhai Shi
    • 1
  1. 1.Department of Agronomy, College of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina

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