Transcriptome and physiological analyses reveal that AM1 as an ABA-mimicking ligand improves drought resistance in Brassica napus
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Abscisic acid (ABA) is the most important stress hormone in the regulation of plant adaptation to drought. Owing to the chemical instability and rapid catabolism of ABA, ABA mimic 1 (AM1) is frequently applied to enhance drought resistance in plants, but the molecular mechanisms governed by AM1 on improving drought resistance in Brassica napus are not entirely understood. To investigate the effect of AM1 on drought resistance at the physiological and molecular levels, exogenous ABA and AM1 were applied to the leaves of two B. napus genotypes (Q2 and Qinyou 8) given progressive drought stress. The results showed that the leaves of 50 µM ABA- and AM1-treated plants shared over 60% differential expressed genes and 90% of the enriched functional pathways in Qinyou 8 under drought. AM1 affected the expression of the genes involved in ABA signaling; they down-regulated pyrabactin resistance/PYR1-like (PYR/PYLs), up-regulated type 2C protein phosphatases (PP2Cs), partially up-regulated sucrose non-fermenting 1-related protein kinase 2s (SnRK2s), and down-regulated ABA-responsive element (ABRE)-binding protein/ABRE-binding factors (AREB/ABFs). Additionally, AM1 treatment repressed the expression of photosynthesis-related genes, those mainly associated with the light reaction process. Moreover, AM1 decreased the stomatal conductance, the net photosynthetic rate, and the transpiration rate, but increased the relative water content in leaves and increased survival rates of two genotypes under drought stress. Our findings suggest that AM1 has a potential to improve drought resistance in B. napus by triggering molecular and physiological responses to reduce water loss and impair growth, leading to increased survival rates.
KeywordsAM1 Transcriptome Drought resistance ABA signaling pathway Photosynthesis Survival rate
ABA-responsive element (ABRE)-binding protein/ABRE-binding factors
Differentially expression genes
Kyoto encyclopedia of genes and genomes
Type 2C protein phosphatase
Pyrabactin resistance 1
Relative water content
Sucrose non-fermenting 1-related protein kinase 2
This work was supported by National Natural Science Foundation of China (Nos. 31571619 and 3151101074). We thanked Prof. Jiankang Zhu from Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences to give us chemical (AM1) support. We also thanked Dr. Naeem MS for his assistance in improving the English of the manuscript.
CLZ designed the experiment. JLX, LLD, and NM conducted the experiment and performed data analysis. JLX wrote the manuscript.
Compliance with ethical standards
Conflict of interest
The authors have no conflicts of interest to this work.
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