The interactions of plant growth regulators and H2O2 during germination improvement of sweet corn seed through spermidine application

  • Yutao Huang
  • Yuchan Zhang
  • Canhong Gao
  • Zhan Li
  • Yajing Guan
  • Weimin Hu
  • Jin Hu
Original paper
  • 2 Downloads

Abstract

Low seed vigor was the main constraint on the production of sweet corn in China. Spermidine (Spd) was proved to enhance sweet corn seed germination. However, little was known about the metabolisms and interactions of plant growth regulators (PGRs) and H2O2 in the enhancement of Spd upon sweet corn seed germination. Spd, GA, C2H4 and H2O2 soaking treatments significantly enhanced seed vigor; while their respective biosynthesis inhibitors and ABA significantly declined seed vigor. Besides, as compared with control, seed vigor showed no significant difference in Spd+ProG (prohexadione-calcium, the inhibitor of GA), however it decreased significantly in Spd+ABA. The seed vigor treated by Spd+AVG (aviglycine hydrochloride, the inhibitor of C2H4) and Spd+NAC (n-acetyl-l-cysteine, a scavenger of H2O2) were significantly lower than those soaked in Spd solution, but still significantly higher than the control. Spd+NAC with significantly lower H2O2 content still up-regulated GA and C2H4 contents and down-regulated ABA content during seed germination. The results suggested that it was Spd rather than H2O2 (produced through Spd) made a direct effect on PGRs metabolism regulation in seed germination enhancement by Spd. The metabolism of GA and ABA played crucial rolesas compared with C2H4 and H2O2. Besides, complicated PGRs interactions and crosstalk between H2O2 and PGRs existed during sweet corn seed germination after Spd soaking, and ABA might be a key hormone in this process.

Keywords

Seed vigor Zea mays H2O2 Plant growth regulation Polyamines Crosstalk 

Notes

Acknowledgements

This study was funded by the National Natural Science Foundation of China (Nos. 31671774, 31371708, 31201279), Zhejiang Provincial Natural Science Foundation (Nos. LY15C130002, LZ14C130002), Dabeinong Funds for Discipline Development and Talent Training in Zhejiang University and Jiangsu Collaborative Innovation Center for Modern Crop Production, P. R. China.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Arc E, Sechet J, Corbineau F, Rajjou L, Marion-Poll A (2013) ABA crosstalk with ethylene and nitric oxide in seed dormancy and germination. Front Plant Sci 4:63PubMedPubMedCentralGoogle Scholar
  2. Argueso CT, Hansen M, Kieber JJ (2007) Regulation of ethylene biosynthesis. J Plant Growth Regul 26:92–105CrossRefGoogle Scholar
  3. Barba-Espín G, Diaz-Vivancos P, Belghazi M, Job C, Hernández J (2011) Understanding the role of H2O2 during pea seed germination: a combined proteomic and hormone profiling approach. Plant Cell Environ 34:1907–1919CrossRefPubMedGoogle Scholar
  4. Bewley JD, Bradford KJ, Hilhorst HWM, Nonogaki H (2013) Seeds: physiology of development, germination and dormancy. Seed Sci Res 23:289CrossRefGoogle Scholar
  5. Cadman CS, Toorop PE, Hilhorst HW, Finch-Savage WE (2006) Gene expression profiles of Arabidopsis Cvi seeds during dormancy cycling indicate a common underlying dormancy control mechanism. Plant J 46:805–822CrossRefPubMedGoogle Scholar
  6. Calvo AP, Nicolas C, Nicolas G, Rodriguez D (2004) Evidence of a cross-talk regulation of a GA20-oxidase (FsGA20ox1) by gibberellins and ethylene during the breaking of dormancy in Fagussylvatica seeds. Physiol Plant 120:623–630CrossRefPubMedGoogle Scholar
  7. Cao DD, Hu J, Gao CH, Guan YJ, Zhang S, Xiao JF (2008) Chilling tolerance of maize (Zea mays L.) can be improved by seed soaking in putrescine. Seed Sci Technol 36:191–197CrossRefGoogle Scholar
  8. Carrera E, Holman T, Medhurst A, Dietrich D, Footitt S, Theodoulou FL, Holdsworth MJ (2008) Seed after-ripening is a discrete developmental pathway associated with specific gene networks in Arabidopsis. Plant J 53:214–224CrossRefPubMedPubMedCentralGoogle Scholar
  9. Deng XP, Xia Y, Hu W, Zhang HX, Shen ZG (2010) Cadmium-induced oxidative damage and protective effects of N-acetyl-l-cysteine against cadmium toxicity in Solanumnigrum, L. J Hazard Mater 180:722–729CrossRefPubMedGoogle Scholar
  10. Finkelstein RR, Gampala SSL, Rock CD (2002) Abscisic acid signaling in seeds and seedlings. Plant Cell 14:S15-S45CrossRefPubMedCentralGoogle Scholar
  11. Finkelstein R, Reeves W, Ariizumi T, Steber C (2008) Molecular aspects of seed dormancy. Plant Bio l59:387CrossRefGoogle Scholar
  12. Fontaine O, Himmelbach A, Yang Y, Grill E (2003) Relay and control of abscisic acid signaling. Curr Opin Plant Bio l6:470–479Google Scholar
  13. Galston AW (1983) Polyamines as modulators of plant development. Bioscience 33:382–388CrossRefGoogle Scholar
  14. Gómez-Jiménez MDC, García-Olivares E, Matilla AJ (2001) 1-Aminocyclopropane-1-carboxylate oxidase from embryonic axes of germinating chick-pea (Cicerarietinum L.) seeds: cellular immunolocalization and alterations in its expression by indole-3-acetic acid, abscisic acid and spermine. Seed Sci Res 11:243–253Google Scholar
  15. Gonzalez ME, Marco F, Minguet EG, Carrasco-Sorli P, Blázquez MA, Carbonell J, RuizOA PieckenstainFL (2011) Perturbation of spermine synthase gene expression and transcript profiling provide new insights on the role of the tetraamine spermine in Arabidopsis thaliana defense against Pseudomonas viridiflava. Plant Physiol 156:2266–2277CrossRefPubMedPubMedCentralGoogle Scholar
  16. Hedden P, Thomas SG (2012) Gibberellin biosynthesis and its regulation. Biochem J 444:11–25CrossRefPubMedGoogle Scholar
  17. Hilhorst HWM (1995) A critical update on seed dormancy. I. Primary dormancy. Seed Sci Res 5:61–73CrossRefGoogle Scholar
  18. Holdsworth MJ, Bentsink L, Soppe WJJ (2008) Molecular networks regulating Arabidopsis seed maturation, afterripening, dormancy and germination. New Phytol 179:33–54CrossRefPubMedGoogle Scholar
  19. Huang AX, She XP, Cao BH, Ren Y (2011) Distribution of hydrogen peroxide during adventitious roots initiation and development in mung bean hypocotyls cuttings. Plant Growth Regul 64:109–118CrossRefGoogle Scholar
  20. Huang YT, Lin C, He F, Li Z, Guan YJ, Hu QJ, Hu J (2017) Exogenous spermidine improves seed germination of sweet corn via involvement in phytohormone interactions, H2O2 and relevant gene expression. BMC Plant Biol 17:1–16CrossRefPubMedPubMedCentralGoogle Scholar
  21. Jones RL (1974) The structure of the lettuce endosperm. Planta1 21:133–146CrossRefGoogle Scholar
  22. Koornneef M, Bentsink L, Hilhorst H (2002) Seed dormancy and germination. Curr Opin Plant Biol 5:33–36CrossRefPubMedGoogle Scholar
  23. Kozarewa I, Cantliffe DJ, Nagata RT, Stoffella PJ (2006) High maturation temperature of lettuce seeds during development increased ethylene production and germination at elevated temperatures. J Am Soc Hortic Sci 131:564–570Google Scholar
  24. Kucera B, Cohn MA, Leubner-Metzger G (2005) Plant hormone interactions during seed dormancy release and germination. Seed Sci Res 15:281–307CrossRefGoogle Scholar
  25. Kuwabara A, Ikegami K, Koshiba T, Nagata T (2003) Effects of ethylene and abscisic acid upon heterophylly in Ludwigiaarcuata (Onagraceae). Planta 217:880–887CrossRefPubMedGoogle Scholar
  26. Leubner-Metzger G (2003) Functions and regulation of b-1,3-glucanase during seed germination, dormancy release and after-ripening. Seed Sci Res 13:17–34CrossRefGoogle Scholar
  27. Leubner-Metzger G, Petruzzelli L, Waldvogel R, Vogeli-Lange R, Meins F (1998) Ethylene-responsive element binding protein (EREBP) expression and the transcriptional regulation of class I b-1,3-glucanase during tobacco seed germination. Plant Mol Biol 38:785–795CrossRefPubMedGoogle Scholar
  28. Li Z, Peng Y, Zhang XQ, Ma X, Huang LK, Yan YH (2014) Exogenous spermidine improves seed germination of white clover under water stress via involvement in starch metabolism, antioxidant defenses and relevant gene expression. Molecules 19:18003–18024CrossRefPubMedGoogle Scholar
  29. Linkies A, Muller K, Morris K, Turekova V, Wenk M, Cadman CSC, Corbineau F, Strnad M, Lynn JR, Finch-Savage WE, Leubner-Metzger G (2009) Ethylene interacts with abscisic acid to regulate endosperm rupture during germination: a comparative approach using Lepidium sativum and Arabidopsis thaliana. Plant Cell 21:3803–3822CrossRefPubMedPubMedCentralGoogle Scholar
  30. Liu Y, Ye N, Liu R, Chen M, Zhang J (2010) H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination. J Exp Bot 61:2979–2990CrossRefPubMedPubMedCentralGoogle Scholar
  31. Luciana P, Immacolata C, Gerhard LM (2000) Ethylene promotes ethylene biosynthesis during pea seed germination by positive feedback regulation of 1-aminocyclo-propane-1-carboxylic acid oxidase. Planta 211:144–149CrossRefGoogle Scholar
  32. Matilla AJ (2000) Ethylene in seed formation and germination. Seed Sci Res 10:111–126CrossRefGoogle Scholar
  33. Nambara E, Marion-PollA (2005) Abscisic acid biosynthesis and catabolism. Annu Rev Plant Biol 56:165–185CrossRefPubMedGoogle Scholar
  34. Nambara E, Okamoto M, Tatematsu K, Yano R, Seo M, Kamiya Y (2010) Abscisic acid and the control of seed dormancy and germination. Seed Sci Res 20:55–67CrossRefGoogle Scholar
  35. Ogawa M, Hanada A, Yamauchi Y, Kuwahara A, Kamiya Y, Yamaguchi S (2003) Gibberellin biosynthesis and response during Arabidopsis seed germination. Plant Cell 15:1591–1604CrossRefPubMedPubMedCentralGoogle Scholar
  36. Peng J, Harberd NP (2002) The role of GA-mediated signaling in the control of seed germination. Curr Opin Plant Bio l5:376–381CrossRefGoogle Scholar
  37. Rajjou L, Duval M, Gallardo K, Catusse J, Bally J, Job C, Job D (2012) Seed germination and vigor. Annu Rev Plant Biol 63:507–533CrossRefPubMedGoogle Scholar
  38. Razem FA, Baron K, Hill RD (2006) Turning on gibberellin and abscisic acid signaling. Curr Opin Plant Biol 9:454–459CrossRefPubMedGoogle Scholar
  39. Saini HS, Consolacion ED, Bassi PK, Spencer MS (1989) Control processes in the induction and relief of thermoinhibition of lettuce seed germination. Actions of phytochrome and endogenous ethylene. Plant Physiol 90:311–315CrossRefPubMedPubMedCentralGoogle Scholar
  40. Strader LC, Ritchie S, Soule JD, McGinnis KM, Steber CM (2004) Recessive-interfering mutations in the gibberellin signaling gene SLEEPY1 are rescued by over-expression of its homologue. SNEEZY Proc Natl Acad Sci 101:12771–12776CrossRefPubMedGoogle Scholar
  41. Subbiah V, Reddy KJ (2010) Interactions between ethylene, abscisic acid and cytokinin during germination and seedling establishment in Arabidopsis. J Biosci 35:451–458CrossRefPubMedGoogle Scholar
  42. Sun TP, Gubler F (2004) Molecular mechanism of gibberellin signaling in plants. Annu Rev Plant Biol 55:197–223CrossRefPubMedGoogle Scholar
  43. Yamaguchi S, Kamiya Y (2002) Gibberellins and light stimulated seed germination. J Plant Growth Regul 20:369–376CrossRefGoogle Scholar
  44. Yamauchi Y, Ogawa M, Kuwahara A, Hanada A, Kamiya Y, Yamaguchi S (2004) Activation of gibberellin biosynthesis and response pathways by low temperature during imbibition of Arabidopsis thaliana seeds. Plant Cell 16:367–378CrossRefPubMedPubMedCentralGoogle Scholar
  45. Zhang S, Hu J, Zhang Y, Xie XJ, Knapp A (2007) Seed priming with brassinolide improves lucerne (Medicago sativa L.) seed germination and seedling growth in relation to physiological changes under salinity stress. Crop Pasture Sci 58:811–815CrossRefGoogle Scholar
  46. Zhang Y, Chen B, Xu Z, Shi Z, Chen S, Huang X, Chen S, Wang X (2014) Involvement of reactive oxygen species in endosperm cap weakening and embryo elongation growth during lettuce seed germination. J Exp Bot 65:3189–3200CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Yutao Huang
    • 1
  • Yuchan Zhang
    • 1
  • Canhong Gao
    • 2
  • Zhan Li
    • 1
  • Yajing Guan
    • 1
  • Weimin Hu
    • 1
  • Jin Hu
    • 1
  1. 1.Seed Science Center, Institute of Crop Science, College of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina
  2. 2.College of AgronomyAnhui Agriculture UniversityHefeiChina

Personalised recommendations