Plant Growth Regulation

, Volume 84, Issue 2, pp 341–349 | Cite as

Quantitative proteomics analysis of proteins involved in leaf senescence of rice (Oryza sativa L.)

  • Xiangbin Xu
  • Kang Guo
  • Wenwen Liang
  • Qifeng Chen
  • Jun Shi
  • Bo Shen
Original paper

Abstract

Delaying leaf senescence and prolonging the available time for photosynthesis is one of the important approaches to increase grain yield of rice (Oryza sativa L.). Here, iTRAQ-based quantitative proteomics approach was used to comparative analyze the expression profiles of proteins in rice leaves in response to senescence. Totally 5067 proteins were identified. Compared with the proteins in the flag leaves at early stage of grain-filling in rice Liang-You-Pei 9 (LYP9), 240 and 188 proteins were up-regulated and down-regulated in the flag leaves at middle stage of grain-filling, and 387 and 202 proteins were up-regulated and down-regulated in the flag leaves at late stage of grain-filling, respectively. In addition, 39 and 18 identified proteins were constantly up-regulated and down-regulated in the leaves from early to middle and late stages of grain-filling, respectively. Among them, chloroplast chaperonin 10, geranylgeranyl diphosphate reductase, Mg chelatase subunit ChLD, porphobilinogen deaminase, protochlorophyllide reductase B and thioredoxin-like protein CITRX might have involved in the senescence of leaves. This study provided important information for understanding the age-sensitive mechanism of LYP9, and offered a foundation for future studying and improving it.

Keywords

Rice iTRAQ Senescence Proteome Chloroplast 

Notes

Acknowledgements

This work is supported by the National Key Technology R&D Program (Grant No. 2011BAD35B02-03), the State Key Laboratory of Rice Biology (Grant No. 120104) and the Major Program of the Zhejiang Province for Food Crop Breeding (Grant No. 2016C02050-6).

Supplementary material

10725_2017_345_MOESM1_ESM.xls (10.9 mb)
Table S1 Profile of all the identified proteins in leaves of rice (XLS 11172 KB)
10725_2017_345_MOESM2_ESM.xls (205 kb)
Table S2 Profile of the up-regulated proteins in leaves at middle stage of grain-filling of LYP9 compared with that in early stage (XLS 205 KB)
10725_2017_345_MOESM3_ESM.xls (160 kb)
Table S3 Profile of the down-regulated proteins in leaves at middle stage of grain-filling of LYP9 compared with that in early stage (XLS 160 KB)
10725_2017_345_MOESM4_ESM.xls (274 kb)
Table S4 Profile of the up-regulated proteins in leaves at late stage of grain-filling of LYP9 compared with that in middle stage (XLS 274 KB)
10725_2017_345_MOESM5_ESM.xls (109 kb)
Table S5 Profile of the down-regulated proteins in leaves at late stage of grain-filling of LYP9 compared with that in middle stage (XLS 109 KB)
10725_2017_345_MOESM6_ESM.xls (21 kb)
Table S6 The constantly up-regulated proteins in the leaves at the early, middle and late stage of grain-filling of LYP9 (XLS 21 KB)
10725_2017_345_MOESM7_ESM.xls (17 kb)
Table S7 The constantly down-regulated proteins in the leaves at the early, middle and late stage of grain-filling of LYP9 (XLS 17 KB)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Xiangbin Xu
    • 1
  • Kang Guo
    • 1
  • Wenwen Liang
    • 1
  • Qifeng Chen
    • 1
  • Jun Shi
    • 1
  • Bo Shen
    • 1
  1. 1.College of Life and Environmental SciencesHangzhou Normal UniversityHangzhouChina

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