Adenosine 5′-monophosphate decreases citrate exudation and aluminium resistance in Tamba black soybean by inhibiting the interaction between 14-3-3 proteins and plasma membrane H+-ATPase
- 62 Downloads
Our previous study suggested that aluminium (Al) stress increased plasma membrane (PM) H+-ATPase activity and citrate secretion and simultaneously enhanced the interaction between 14-3-3 proteins and phosphorylated PM H+-ATPase in Al-resistant Tamba black soybean (RB). Adenosine 5′-monophosphate (AMP) is known as an inhibitor of the interaction between 14-3-3 proteins and PM H+-ATPases. To investigate the effects of AMP on Al resistance, PM H+-ATPase activity and citrate exudation, AMP was used to treat Al-stressed RB. The results showed that after treatment with either 100 μM AMP or 50 μM Al for 8 h, RB root growth was inhibited by approximately 50 and 30%, respectively. However, simultaneous treatment with 100 μM AMP and 50 μM Al for 8 h resulted in a 60% inhibition of RB root growth, indicating that the presence of AMP reduced Al tolerance in RB. The interaction of PM H+-ATPase and 14-3-3 proteins in the root tips of Al-treated RB was stronger than that in the untreated control. However, the interaction of the two proteins was greatly reduced (lower than that in the control) after co-treatment with Al and AMP, suggesting that the presence of AMP under Al stress reduced the Al-enhanced interaction between PM H+-ATPase and 14-3-3 proteins. Consequently, PM H+-ATPase activity decreased by approximately 50%, which led to a significant decrease in H+ efflux and citrate secretion in RB roots under Al stress. Collectively, these results indicate that AMP reduced citrate exudation and Al resistance in RB by inhibiting the interaction between 14-3-3 proteins and PM H+-ATPases under Al stress.
KeywordsAl stress Tamba black soybean Plasma membrane H+-ATPase activity Adenosine 5′-monophosphate Citrate secretion
This work was supported in part by the grant of the National Natural Science Foundation of China (No. 31260063), the grant of the National Basic Research Program of China (No. 2014CB138701) and by the grant of Yunnan Applied Basic Research Projects of China (No. 2013FZ120). We are grateful for the instrument assistance of Prof Kunzhi Li, Kunming University of Science and Technology for this study.
- Chen Q, Guo CL, Wang P, Chen XQ, Wu KH, Li KZ, Yu YX, Chen LM (2013) Up-regulation and interaction of the plasma membrane H+-ATPase and the 14-3-3 protein are involved in the regulation of citrate exudation from the broad bean (Vicia faba L.) under Al stress. Plant Physiol Bioch 70:504–511CrossRefGoogle Scholar
- Kaiser WM, Huber S (1994) Modulation of nitrate reductase in vivo and in vitro: effects of phosphoprotein phosphatase inhibitors, free Mg2 + and 5′-. AMPPlanta 193:358–364Google Scholar
- Reggiani R, Mattana M, Aurisano N, Bertani A (1993) Utilization of stored nitrate during the anaerobic germination of rice seeds. Plant Cell Physiol 34:379–383Google Scholar
- Sabina RL, Patterson D, Holmes EW (1985) 5-Amino-4-imidazolecarboxamide riboside (Z-riboside) metabolism in eukaryotic cells. J Bio Chem 260:6107–6114Google Scholar
- Shen H, He L, Sasaki T, Yamamoto Y, Zheng S, Ligaba A, Yan X, Ahn S, yamaguchi M, Sasakawa H, Matsumoto H (2005) Citrate secretion coupled with the modulation of soybean root tip under aluminum stress: up-regulation of transcription, translation, and threonine-oriented phosphorylation, of plasma membrane H+-ATPase. Plant Physiol 138:287–296CrossRefPubMedPubMedCentralGoogle Scholar