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Plant Growth Regulation

, Volume 84, Issue 2, pp 237–248 | Cite as

Further insight into the role of KAN1, a member of KANADI transcription factor family in rice

  • Yawo Mawunyo Nevame Adedze
  • Baobing Feng
  • Lei Shi
  • Zhonghua Sheng
  • Shaoqing Tang
  • Xiangjin Wei
  • Peisong Hu
Original paper
  • 293 Downloads

Abstract

The rice EMS-derived mutant leaf adaxialized 1 (lad1) was isolated based on its upward rolling leaf phenotype. Besides the adaxially rolled leaf, many other agronomic traits were also compromised in lad1. The rolling trait was characterized by a noticeable alteration of bulliform cells in the adaxial side of the leaves. Map-based cloning showed a single nucleotide substitution in the promoter region of the KAN1 gene in lad1 mutant. Further, over-expressing and CRISPR/cas9-edited knockdown transgenic plants confirmed that KAN1 was responsible for the mutant phenotype of lad1. Yeast two-hybrid and bimolecular fluorescence complementation assay demonstrated that KAN1 can interact with the auxin response factors ARF3, ARF7 and ARF15. Physiologically, the contents of auxin (IAA), abscisic acid (ABA), jasmonic acid (JA) and gibberellin (GA) were all significantly increased in the lad1 mutant. Moreover, the GA3 content dramatically decrease in wild-type, but increased in lad1 under IAA induction. Additionally, the expression levels of several IAA and GA biosynthesis and responsive-related genes and genes involved in leaf polarity determination were altered in lad1. Therefore, we hypothesized that KAN1/ARFs protein complexes act as auxin-dependent regulatory units that play a conserved role in leaf development.

Keywords

Auxin response transcription factors KANADI transcription factor Leaf adaxialized 1 Leaf rolling Phytohormones 

Notes

Acknowledgements

This work was supported by the National Key Research and Development Program of China (2016YFD0101801), the National Natural Science Foundation of China (31501285, 31521064), and by the National S&T Major Project of China (2016ZX08001006).

Author contributions

YMNA, BF and LS performed the experiments. YMNA and XW analyzed the data. PH and XW designed the project. YMNA and XW draft the manuscript. ZS and ST performed a critical revision of the article. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

10725_2017_335_MOESM1_ESM.pdf (929 kb)
Supplementary material 1 (PDF 929 KB)

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Yawo Mawunyo Nevame Adedze
    • 1
  • Baobing Feng
    • 1
  • Lei Shi
    • 1
  • Zhonghua Sheng
    • 1
  • Shaoqing Tang
    • 1
  • Xiangjin Wei
    • 1
  • Peisong Hu
    • 1
  1. 1.State Key Laboratory of Rice BiologyChina National Rice Research InstituteHangzhouPeople’s Republic of China

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