OsSNAP32, a SNAP25-type SNARE protein-encoding gene from rice, enhanced resistance to blast fungus
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SNAREs play important roles in plant responses to various biotic and abiotic stresses. OsSNAP32 is a SNAP25-type SNARE protein-encoding gene isolated from rice. OsSNAP32 is ubiquitously expressed in the various tissues of blast-resistant rice landrace Heikezijing and blast-susceptible Suyunuo, with higher expression in Heikezijing and induced expression in rice seedlings inoculated by the blast pathogen (Magnaporthe oryzae) race Hoku1. OsSNAP32-overexpressing transgenic lines in Suyunuo increased resistance to blast, with fewer lesions in the inoculated leaves compared with the non-transgenic control Suyunuo. OsSNAP32 RNAi transgenic lines in Heikezijing decreased resistance to blast, with some typical lesions in the inoculated leaves compared with the non-transgenic control Heikezijing. These results suggested that OsSNAP32 might be involved in rice resistance to blast.
KeywordsOryza sativa L. OsSNAP32 Magnaporthe oryzae Transgenic rice Resistance
Days past inoculation
Hours past inoculation
Hexadecyl trimethyl ammonium bromide
This research has been supported by Grants the National Key Project for Transgenic Crops (2014ZX08009-001B, 2014ZX08009-003-001-010), the Natural Science Foundation of China (30900888 and 31171516), the Fundamental Research Funds for the Central Universities (KYZ201302), Jiangsu Agriculture science and technology innovation fund (CX(12)1003-3, CX(15)1054). We wish to thank Prof. Zhongzhuan Lin, CAAS, China for providing the Japanese blast race Hoku1 and Prof. Zhiyi Chen, Prof. Yongfeng Liu, JAAS, China for providing six Chinese blast races, i.e., 191ZB13, 97-2ZC15, 35-1ZD1, 55-1ZE3, 42-2ZF1 and 113ZG1.
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