An analysis of transcripts and enzyme profiles in drought stressed jute (Corchorus capsularis) and rice (Oryza sativa) seedlings treated with CaCl2, hydroxyapatite nano-particle and β-amino butyric acid
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In the following work we have identified and isolated transcripts induced upon treatment of jute (JRC 412) seeds with as elicitors through the differential display analysis. We have tried an approach of pre-treatment of jute seeds with CaCl2 (calcium chloride), CaNP (hydroxyapatite nano-particle) and BABA (β-amino butyric acid) as elicitors to study the changes in both biochemical parameters and transcriptomic changes. Rice was chosen as the biological replicate model for the enzymatic changes involved. Several genes including the Late Embryogenesis Abundant protein, Dehydration Responsive Element along with the biochemical markers like proline and peroxidase associated with the priming and Systemic Acquired Resistance of the jute seedlings were found to be up-regulated under the effect of the elicitors. The significance of the isolated differentially expressed genes has been discussed in the light of these findings and there was distinct similarity in the enzyme profile that suggested that biosynthesis and control of proline levels in stressed plants was central to the survival strategy.
KeywordsDrought Elicitors CaCl2 (calcium chloride) CaNP (hydroxyapatite nano-particle) BABA (β-amino butyric acid) DD (differential display) SAR (Systemic Acquired Resistance)
This work has been funded by the Department of Biotechnology (WB-DBT) West Bengal, India and supported by the Council of Scientific and Industrial Research (CSIR), University Grants Commission (UGC) India, and the DBT-IPLS, University of Calcutta.
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