Plant Growth Regulation

, Volume 79, Issue 3, pp 319–330 | Cite as

Effects of exogenous putrescine on glycolysis and Krebs cycle metabolism in cucumber leaves subjected to salt stress

  • Min Zhong
  • Yinghui Yuan
  • Sheng Shu
  • Jin Sun
  • Shirong Guo
  • Ruonan Yuan
  • Yuanyuan Tang
Original paper


The effects of exogenous putrescine (8 mmol L−1) on the carbohydrate content, glycolytic metabolism, citric acid cycle intermediates, key enzyme activities and their corresponding gene expression in cucumber (cucumis sativus L. cv. Jingyou NO.4) subjected to salt stress (75 mmol L−1 NaCl) in solution culture was studied. Phosphofructokinase (PFK), pyruvate kinase (PK) and phosphoenolpyruvate pyruvate kinase (PEPC) activities were significantly decreased in leaves when exposed to salt stress. NaCl stress caused the PFK and PK gene expression and the content of pyruvate to significantly decrease, while PEPC gene expression increased. Salt stress also significantly reduced isocitrate dehydrogenase, malate dehydrogenase and succinate dehydrogenase and their levels of transcription, causing decreases in the citric acid, succinic acid and malic acid content in leaves. Exogenous putrescine reversed the salt stress and increased gene expression and, in turn, the key enzymes involved in the glycolysis pathway and Krebs cycle, which significantly increased the amounts of organic acids and pyruvate produced, promoting the release of more energy currency (ATP and ADP). These results suggest that Put effectively participate glycolysis pathway and Krebs cycle, reducing the excessive accumulation of carbohydrates in the leaves, and providing more material and energy for the defense salt-induced injury, thus enhances cucumber seedling to salt stress tolerance.


Cucumber Putrescine Salt stress Glycolysis pathway Krebs cycle Gene expression 



This work was financially supported by the National Natural Science Foundation of China (Nos. 31471869, 31401919 and 31272209), the China Postdoctoral Science Foundation Funded Project (2014M561665), the China Earmarked Fund for Modern Agro-industry Technology Research System (CARS-25-C-03), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and sponsored by the Research Fund for the Doctoral Program of Higher Education (20130097120015)..

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10725_2015_136_MOESM1_ESM.docx (1.7 mb)
Supplementary material 1 (DOCX 1767 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Min Zhong
    • 1
  • Yinghui Yuan
    • 1
  • Sheng Shu
    • 1
  • Jin Sun
    • 1
    • 2
  • Shirong Guo
    • 1
    • 2
  • Ruonan Yuan
    • 1
  • Yuanyuan Tang
    • 1
  1. 1.Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, College of HorticultureNanjing Agricultural UniversityNanjingChina
  2. 2.Nanjing Agricultural University (Suqian) Academy of Protected HorticultureSuqianChina

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