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Plant Growth Regulation

, Volume 77, Issue 2, pp 133–146 | Cite as

Proteomic analysis of salt-stress responsive proteins in roots of tomato (Lycopersicon esculentum L.) plants towards silicon efficiency

  • Sowbiya Muneer
  • Byoung Ryong Jeong
Original paper

Abstract

Salt stress is a rising threat to crop productivity, among several notable consumed crops tomato is often under threat due to salt stress. In this study, the role of Si in restraining salinity stress responses in root proteome and genes involved in stress tolerance has been studied. Fifteen days old tomato (Lycopersicon esculentum L.) plants grown hydroponically with or without NaCl were fed with 2.5 mM Si in the form of potassium silicate [K2SiO3]. The response to a combined effect of NaCl and Si were studied 5 days after treatment. Proteomic analysis indicated that 40 proteins were differentially expressed under Si and/or salt stress treatments. Twenty-four of them were up-regulated by Si supplements (50 mM +NaCl/+Si) and down-regulated in salt-stressed roots (50 mM +NaCl/−Si), and these proteins were mostly associated with stress responses, plant hormones and transcriptional regulations. The rest of them belong to other secondary metabolites. Moreover, 17 differentially expressed proteins (25 mM +NaCl/+Si), up-regulated in the Si treatments, were mostly related to stress responses, plant hormones and cellular biosynthesis, and the rest of the proteins were related to transcriptional regulation, RNA binding and other secondary metabolisms. In addition, 17 protein spots were observed absent in salinity-stressed roots (25/or 50 mM +NaCl/−Si). Moreover, the important genes associated with salt stress responses (leDREB-1, leDREB-2 and leDREB-3), antioxidants (leAPX, leSOD and leCAT genes) and Si transport (leLsi-1, leLsi-2 and leLsi-3) were analyzed by the real-time polymerase chain reaction. The physiological data such as thiobarbituric acid reactive substances, superoxide dismutase as an oxidative stress marker and concentration of Si all correlated well with proteomic and gene expression data. The observed responses to Si supply in salt stressed plants indicate that the Si has a substantial role in alleviating the salinity stress responses by improving the root proteome and activating important genes responsible for stress tolerance.

Keywords

Antioxidant-genes DREB-genes Lsi-genes Lycopersicon esculentum Proteomics Salt stress Silicon 

Abbreviations

DREB

Dehydrative response element

Lsi

Low silicon

SOD

Superoxide dismutase

APX

Ascorbate peroxidase

CAT

Catalase

IEF

Isoelectric focusing

IPG

Immobilized pressure gradient

NL

Nonlinear

2-DE

Second dimension electrophoresis

SDS

Sodium dodecyl sulfate

MS

Mass spectrometer

MALDI-TOF

Matrix assisted laser desorption/ionization time of flight

Si

Silicon

NaCl

Sodium chloride

PCR

Polymerase chain reaction

Notes

Acknowledgments

This study was supported from the grants of BK21 Plus program (Brain Korea 21), Ministry of Education, South Korea.

Conflict of interest

Authors declare no conflict of interest.

Supplementary material

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Supplementary material 1 (PPTX 3761 kb)
10725_2015_45_MOESM2_ESM.docx (13 kb)
Supplementary material 2 (DOCX 13 kb)
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Supplementary material 3 (XLSX 10 kb)
10725_2015_45_MOESM4_ESM.xlsx (11 kb)
Supplementary material 4 (XLSX 10 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Division of Applied Life Science (BK21 Plus), Graduate SchoolGyeongsang National UniversityJinjuSouth Korea
  2. 2.Institute of Agriculture and Life ScienceGyeongsang National UniversityJinjuSouth Korea
  3. 3.Research Institute of Life ScienceGyeongsang National UniversityJinjuSouth Korea

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