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Plant Growth Regulation

, Volume 74, Issue 1, pp 65–71 | Cite as

Differential gene expression profiles in latex from Hevea brasiliensis between self-rooting juvenile and donor clones

  • Hui-Liang Li
  • Dong Guo
  • Shi-Qing Peng
Original paper

Abstract

Suppression subtractive hybridization method was used to profile gene changes in the latex between self-rooting juvenile clones (JCs) and donor clones (DCs) to better understand the molecular mechanism underlying the differences between these clones. 176 differentially expressed genes were identified, 95 of which were upregulated and 81 with reduced self-rooting JCs. These genes were classified as stress/defense response, rubber biosynthesis, metabolism and energy, protein metabolism, signal transduction transcription, and post-transcription. Systematic analyses of the differentially expressed genes between self-rooting JCs and DCs suggest that rubber biosynthesis, production, and scavenging of reactive oxygen species may have significant functions in high-yielding self-rooting JCs. This result contributes to better understanding on the molecular mechanism underlying the difference between self-rooting JCs and DCs.

Keywords

Differential gene Hevea brasiliensis Latex self-rooting juvenile clone 

Abbreviations

DCs

Donor clones

HMGR-CoA

3-Hydroxy-3-methylglutaryl coenzyme A reductase

JCs

Juvenile clones

ROS

Reactive oxygen species

REF

Rubber elongation factor

SRPP

Small rubber particle protein

SSH

Suppression subtractive hybridization

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Nos. 31170634), Hainan Sciences and Technology Major Projects (ZDZX2013023-1) and a Chinese National Nonprofit Institute Research Grant of CATAS-ITBB (ITBB110205). We deeply thank Professor Xiong-Ting Chen (Institute of Tropical Biosciences and Biotechnology, CATAS) for his collaboration in treatment of plant materials.

Supplementary material

10725_2014_9900_MOESM1_ESM.xls (68 kb)
Supplementary material 1 (XLS 68 kb)
10725_2014_9900_MOESM2_ESM.xls (62 kb)
Supplementary material 2 (XLS 61 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikouChina

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