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Plant Growth Regulation

, Volume 76, Issue 1, pp 61–70 | Cite as

Comparison of vitality between seedlings germinated from black-coated and yellow-coated seeds of a turnip rape (Brassica rapa L.) subjected to NaCl and CdCl2 stresses

  • Lijie Xuan
  • Nazim Hussain
  • Zhong Wang
  • Yuxiao Jiang
  • Mingxun Chen
  • Lixi Jiang
Original Paper

Abstract

Yellow-seeded (YS) rapeseed varieties have attracted considerable interests from cultivators because of their thin seed coat and high seed oil content. However, compared with black-seeded (BS) rapeseed, little is known about the response of YS rapeseed to abiotic stresses. In this study, we characterized the cellular structures of YS varieties and BS varieties and the physiological parameters of the YS and BS seedlings subjected to high-salt and/or high-cadmium conditions. We observed larger and denser (in arrangement) oilbodies in YS than in BS varieties. The BS variety seed coat was much thicker than that of the YS variety because of the existence of a palisade layer where pigments are deposited. Either at the eighth day or 1 month after sowing, YS seedlings showed higher sensitivity to NaCl and/or CdCl2 stress than BS seedlings, as reflected by the length of roots, biomass, and a variety of physiological parameters, including MDA, chlorophyll content, and antioxidant activities. Our results suggested that the more vigorous growth of BS seedlings is likely due to the higher flavonoid content in their vegetative tissues, and the poor performance of YS seedlings under stress treatment (especially with NaCl) could be attributed to its relatively low flavonoid content. Our findings raise some points that need further investigation to obtain an in-depth understanding of the molecular mechanisms involved.

Keywords

Yellow seeded rapeseeds Abiotic stress Seed oil content Brassica rapa 

Abbreviations

YS

Yellow seeded

BS

Black seeded

PAs

Proanthocyanidins

TT

Transparent Testa

TAGs

Triacylglycerols

SOD

Superoxide dismutase

POD

Peroxide

MDA

Malondialdehyde

Notes

Acknowledgments

The work of our lab was sponsored by the National Key Basic Research Project (abbreviated as 973 project, Code No. 2015CB150205) and Jiangsu Collaborative Innovation Center for Modern Crop Production. We thank Miss Mei Li for her technical assistance.

Supplementary material

10725_2014_19_MOESM1_ESM.pptx (4.9 mb)
Supplementary material 1 (PPTX 4977 kb)
10725_2014_19_MOESM2_ESM.doc (88 kb)
Supplementary material 2 (DOC 88 kb)
10725_2014_19_MOESM3_ESM.docx (12 kb)
Supplementary material 3 (DOCX 13 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.College of Agriculture and BiotechnologyZhejiang UniversityHangzhouPeople’s Republic of China

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