Comparison of vitality between seedlings germinated from black-coated and yellow-coated seeds of a turnip rape (Brassica rapa L.) subjected to NaCl and CdCl2 stresses
- 376 Downloads
Yellow-seeded (YS) rapeseed varieties have attracted considerable interests from cultivators because of their thin seed coat and high seed oil content. However, compared with black-seeded (BS) rapeseed, little is known about the response of YS rapeseed to abiotic stresses. In this study, we characterized the cellular structures of YS varieties and BS varieties and the physiological parameters of the YS and BS seedlings subjected to high-salt and/or high-cadmium conditions. We observed larger and denser (in arrangement) oilbodies in YS than in BS varieties. The BS variety seed coat was much thicker than that of the YS variety because of the existence of a palisade layer where pigments are deposited. Either at the eighth day or 1 month after sowing, YS seedlings showed higher sensitivity to NaCl and/or CdCl2 stress than BS seedlings, as reflected by the length of roots, biomass, and a variety of physiological parameters, including MDA, chlorophyll content, and antioxidant activities. Our results suggested that the more vigorous growth of BS seedlings is likely due to the higher flavonoid content in their vegetative tissues, and the poor performance of YS seedlings under stress treatment (especially with NaCl) could be attributed to its relatively low flavonoid content. Our findings raise some points that need further investigation to obtain an in-depth understanding of the molecular mechanisms involved.
KeywordsYellow seeded rapeseeds Abiotic stress Seed oil content Brassica rapa
The work of our lab was sponsored by the National Key Basic Research Project (abbreviated as 973 project, Code No. 2015CB150205) and Jiangsu Collaborative Innovation Center for Modern Crop Production. We thank Miss Mei Li for her technical assistance.
- Dong JS, Shi DQ, Gao JQ, Li CL, Liu J, Qi CK, Yang WC (2009) Correlation between the quantity and the sum of areas of oil bodies and oil content in rapeseed (Brassica napus). Chin Bull Bot 44:79–85Google Scholar
- Lauchli A (1990) Calcium, salinity and the plasma membrane. In: Leonard RT, Hepler PK (eds) Calcium in plant growth and development. American Society of Plant Physiologists, Rockville, pp 26–35Google Scholar
- Qu C, Fu F, Lu K, Zhang K, Wang R, Xu X, Wang M, Lu J, Wan H, Zhanglin T, Li J (2013) Differential accumulation of phenolic compounds and expression of related genes in black- and yellow-seeded Brassica napus. J Exp Bot 64(10):2885–2898. doi: 10.1093/jxb/ert148 CrossRefPubMedCentralPubMedGoogle Scholar
- Sarry JE, Kuhn L, Ducruix C, Lafaye A, Junot C, Hugouvieux V, Jourdain A, Bastien O, Fievet JB, Vaihen D, Amekraz B, Moulin C, Ezan E, Garin J, Bourguignon J (2006) The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses. Proteomics 6(7):2180–2198CrossRefPubMedGoogle Scholar
- Velasco L, Fernández-Martínez JM, Haro AD (1997) Determination of the fatty acid composition of the oil in intact-seed mustard by near-infrared reflectance spectroscopy. JAOCS 74(12):1595–1602Google Scholar
- Zhu YN, Cao ZY, Xu F, Huang Y, Chen MX, Guo WL, Zhou WJ, Zhu J, Meng JL, Zou J, Jiang LX (2012) Analysis of gene expression profiles of two near-isogenic lines differing at a QTL region affecting oil content at high temperatures during seed maturation in oilseed rape (Brassica napus L.). TAG 124:515–531CrossRefPubMedGoogle Scholar