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Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings

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Abstract

Theanine synthetase (TS) is a key enzyme involved in theanine biosynthesis. In our recent study, it has been revealed that theanine biosynthesis derived from nitrogen metabolism in tea (Camellia sinensis) plants can be influenced by shading treatment. The expression patterns of CsTS protein in the roots and shoots of tea seedlings were examined by western blot using a self-prepared polyclonal antibody with high specificity and sensitivity. The effect of long-term shade treatment on the levels of theanine synthesis was also investigated using roots and shoots of tea seedlings. Levels of theanine and total free amino acids gradually increased in shoots, reaching their maximum after 22 days of treatment (DOT). The immunoblotting analysis suggested that CsTS protein levels increased gradually up to 22 DOT and expression remained at a high level, except after 1 DOT where levels were low in both roots and shoots. The increased theanine concentration we observed in the shading treatment may be due to increased nitrogen assimilation and reduced theanine catabolism under shade conditions.

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References

  • Crozier A, Yokota T, Jaganath IB, Marks SC, Saltmarsh M, Clifford MN (2006) Secondary metabolites in fruits, vegetables, beverages and other plant-based dietary components. In: Crozier A, Clifford MN, Ashihara H (eds) Plant secondary metabolites—occurrence, structure and role in the human diet. Blackwell, Oxford, pp 208–302

    Chapter  Google Scholar 

  • Deng WW, Ashihara H (2010) Profiles of purine metabolism in leaves and roots of Camellia sinensis seedlings. Plant Cell Physiol 51:2105–2118

    Article  PubMed  CAS  Google Scholar 

  • Deng WW, Ogita S, Ashihara H (2008) Biosynthesis of theanine (γ-ethylamino-l-glutamic acid) in seedlings of Camellia sinensis. Phytochem Lett 1:115–119

    Article  CAS  Google Scholar 

  • Deng WW, Wang S, Chen Q, Zhang ZZ, Hu XY (2012) Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings. Plant Physiol Biochem 56:35–40

    Article  PubMed  CAS  Google Scholar 

  • Jani D, Meena LS, Rizwan-ul-Haq QM, Singh Y, Sharma AK, Tyagi AK (2002) Expression of cholera toxin B subunit in transgenic tomato plants. Transgenic Res 11:447–454

    Article  PubMed  CAS  Google Scholar 

  • Kito M, Kokura H, Izaki J, Sadaoka K (1968) Theanine, a precursor of the phloroglucinol nucleus of catechins in tea plants. Phytochemistry 7:599–603

    Article  CAS  Google Scholar 

  • Kotaniguchi H, Kawakatsu M, Toyo’oka T, Imai K (1987) Automatic amino acid analysis utilizing 4-fluoro-7-nitrobenzo-2-oxa-1, 3-diazole. J Chromatogr 420:141–145

    PubMed  CAS  Google Scholar 

  • Ku K, Choi JN, Kim J, Kim JK, Yoo LG, Lee SJ, Hong YS, Lee CH (2010) Metabolomics analysis reveals the compositional differences of shade grown tea (Camellia sinensis L.). J Agric Food Chem 58:418–426

    Article  PubMed  CAS  Google Scholar 

  • Lam HM, Coschigano KT, Oliveira IC, Melo-Oliveira R, Coruzzi GM (1996) The molecular-genetics of nitrogen assimilation into amino acids in higher plants. Annu Rev Plant Physiol 47:569–593

    Article  CAS  Google Scholar 

  • Okano K, Chutani K, Matuso K (1997) Suitable level of nitrogen fertilizer for tea (Camellia sinensis L.) plants in relation to growth, photosynthesis, nitrogen uptake and accumulation of free amino acids. Jpn J Crop Sci 66:279–287

    Article  CAS  Google Scholar 

  • Sakai S (1975) Recent studies and problems of photosynthesis of tea plant. JARQ 9:101–106

    Google Scholar 

  • Sakato Y (1949) The chemical constituents of tea. III. A new amide theanine (in Japanese). Nippon Nogeikagaku Kaishi 23:262–267

    Article  Google Scholar 

  • Takeo T (1981) Nitrogen metabolism pertaining to biosynthesis of theanine in tea plants. JARQ 15:110–116

    Article  CAS  Google Scholar 

  • Tian L, Kong WF, Pan QH, Zhan JC, Wen PF, Chen JY, Wan SB, Huang WD (2006) Expression of the chalcone synthase gene from grape and preparation of an anti-CHS antibody. Protein Expres Purif 50:223–228

    Article  CAS  Google Scholar 

  • Tsushida T, Takeo T (1984) Ethylamine content of fresh tea shoots and made tea determined by high performance liquid chromatography. J Sci Food Agric 35:77–83

    Article  CAS  Google Scholar 

  • Vance NC, Zaerr JB (1990) Analysis by high-performance liquid chromatography of free amino acids extracted from needles of drought-stressed and shaded. Physiol Plantarum 79:23–30

    Article  CAS  Google Scholar 

  • Wojciechowska R, Siwek P (2006) The effect of shading on nitrate metabolism in stalks and blades of celery leaves (Apium graveolens L. var. dulce). Folia Horticulturae 18:25–35

    Google Scholar 

  • Yamaguchi S, Ninomiya K (2000) Umami and food palatability. J Nutr 130:921–926

    Google Scholar 

  • Zhang WJ, Liang YR, Zhang FZ, Chen CS, Zhang YG, Chen RB, Weng BQ (2004) Effects on the yield and quality of oolong tea by covering with shading. net. J Tea Sci 24:276–282 (in Chinese)

    Google Scholar 

Download references

Acknowledgments

This research was supported by the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT1101) and Natural Science Foundation of China (No. 31170283).

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Authors and Affiliations

  1. Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Education and Ministry of Agriculture, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, Anhui, China

    Wei-Wei Deng, Yue Fei, Shuo Wang, Xiao-Chun Wan & Zheng-Zhu Zhang

  2. Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Institute of Tibet Plateau Research at Kunming, Chinese Academy of Sciences, Kunming, 650204, China

    Xiang-Yang Hu

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  1. Wei-Wei Deng
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  2. Yue Fei
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  3. Shuo Wang
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  4. Xiao-Chun Wan
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  5. Zheng-Zhu Zhang
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  6. Xiang-Yang Hu
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Correspondence to Zheng-Zhu Zhang.

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Deng, WW., Fei, Y., Wang, S. et al. Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings. Plant Growth Regul 71, 295–299 (2013). https://doi.org/10.1007/s10725-013-9828-1

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