Plant Growth Regulation

, Volume 71, Issue 3, pp 295–299 | Cite as

Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings

  • Wei-Wei Deng
  • Yue Fei
  • Shuo Wang
  • Xiao-Chun Wan
  • Zheng-Zhu Zhang
  • Xiang-Yang Hu
Brief communication


Theanine synthetase (TS) is a key enzyme involved in theanine biosynthesis. In our recent study, it has been revealed that theanine biosynthesis derived from nitrogen metabolism in tea (Camellia sinensis) plants can be influenced by shading treatment. The expression patterns of CsTS protein in the roots and shoots of tea seedlings were examined by western blot using a self-prepared polyclonal antibody with high specificity and sensitivity. The effect of long-term shade treatment on the levels of theanine synthesis was also investigated using roots and shoots of tea seedlings. Levels of theanine and total free amino acids gradually increased in shoots, reaching their maximum after 22 days of treatment (DOT). The immunoblotting analysis suggested that CsTS protein levels increased gradually up to 22 DOT and expression remained at a high level, except after 1 DOT where levels were low in both roots and shoots. The increased theanine concentration we observed in the shading treatment may be due to increased nitrogen assimilation and reduced theanine catabolism under shade conditions.


Camellia sinensis Theanine synthetase Shade Theanine Amino acid 



This research was supported by the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT1101) and Natural Science Foundation of China (No. 31170283).

Supplementary material

10725_2013_9828_MOESM1_ESM.pdf (372 kb)
Supplementary material 1 (PDF 372 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Wei-Wei Deng
    • 1
  • Yue Fei
    • 1
  • Shuo Wang
    • 1
  • Xiao-Chun Wan
    • 1
  • Zheng-Zhu Zhang
    • 1
  • Xiang-Yang Hu
    • 2
  1. 1.Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Education and Ministry of AgricultureAnhui Agricultural UniversityHefeiChina
  2. 2.Key Laboratory of Biodiversity and BiogeographyKunming Institute of Botany, Institute of Tibet Plateau Research at Kunming, Chinese Academy of SciencesKunmingChina

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