Plant Growth Regulation

, Volume 68, Issue 3, pp 383–393 | Cite as

Maize (Zea mays L.) homologue of ABA-insensitive (ABI) 5 gene plays a negative regulatory role in abiotic stresses response

  • Fang Yan
  • Wei Deng
  • Xiaomin Wang
  • Chunwen Yang
  • Zhengguo Li
Original paper


Abscisic acid serves as an important hormone involved in response to environmental stresses. In this study, a homolog of ABA-insensitive (ABI) 5 gene, named as ZmABI5, has been isolated from maize (Zea mays L.). Expression of ZmABI5 is induced by a wide spectrum of stresses, including abscisic acid, salicylic acid, NaCl, high and low temperature, wounding and mannitol treatments. To identify the function of the ZmABI5 under stress conditions, a binary vector containing ZmABI5 driven by CaMV 35S promoter was constructed and the transgenic tobacco (Nicotiana tabacum) plants over-expressing ZmABI5 were produced by Agrobacterium-mediated transformation. When treated with NaCl, mannitol, high and low temperature, the transgenic plants display obvious stress-sensitive phenotypes. The activity of superoxide dismutase and peroxidase, content of proline in the transgenic plants were obviously lower than that of wild type plants. Malondialdehyde content was higher in the transgenic plants than that of wild type plants. Quantitative PCR analysis showed that over-expression of ZmABI5 altered the expression of GCC box-containing gene PR5, and drought-responsive element/C-repeat (DRE/CRT) genes (CAT1, APX and NtERD10A, B, C, D). These results demonstrate that over-expression of ZmABI5 makes the tobacco plants sensitive to salt, drought, high and low temperature stresses. It suggests that ZmABI5 plays a negative regulatory role in stresses response.


ABI5 Over-expression Stress Tobacco Tolerance 



Abscisic acid




ABA-responsive element


Basic leucine zipper


Dehydration responsive element


Quantitative RT-PCR


Reactive oxygen species


Salicylic acid



This study was supported by the Fundamental Research Funds for the Central Universities of China (CDJXS102300).


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Fang Yan
    • 1
    • 2
  • Wei Deng
    • 1
    • 2
  • Xiaomin Wang
    • 1
    • 2
  • Chunwen Yang
    • 1
    • 2
  • Zhengguo Li
    • 1
    • 2
  1. 1.Genetic Engineering Research Center, Bio-Engineering CollegeChongqing UniversityChongqingChina
  2. 2.Key Lab of Functional Gene and New Regulation Technologies Under Chongqing Municipal Education CommissionChongqingChina

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