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Plant Growth Regulation

, Volume 67, Issue 3, pp 281–304 | Cite as

Regulation of metabolomics in Atriplex halimus growth under salt and drought stress

  • Mamdouh M. Nemat Alla
  • Abdel-Hamid A. Khedr
  • Mamdouh M. Serag
  • Amina Z. Abu-Alnaga
  • Reham M. Nada
Original paper

Abstract

Forty-day-old Atriplex halimus seedlings were treated with either NaCl (50, 300 and 550 mM) for the subsequent 30 days or PEG for the following 3, 6 and 10 days. Shoot fresh and dry weights were significantly increased by 50 mM NaCl; nevertheless, the other concentrations had no effect. However, the growth was reduced by drought only after 10 days. Meanwhile, Na+ was accumulated in treated plants; the magnitude of accumulation was highest with high NaCl concentration or PEG for 10 days. The metabolite profiles showed discrimination particularly up-regulation of the amino acids proline, valine, isoleucine, and methionine. Moreover, the macro analysis revealed that NaCl- and PEG-treated plants shared 10 % of the metabolites in the positive mode, however, 87 % were unique to NaCl and 46 % were unique to PEG whereas in the negative mode, 8 % were in share while 90 or 53 % were restricted to NaCl or PEG, respectively. Additionally, sucrose in particular was significantly increased up to threefold and fivefold by 300 and 550 mM NaCl, respectively and up to 2.5-fold by drought for 10 days, nevertheless, the other sugar fractions remained largely unchanged. Also, proline was significantly increased by only the high NaCl concentrations and the long-term drought, nonetheless, the other treatments led, if any, to decreases. These results conclude that NaCl affects the metabolite profiles more than PEG and these metabolites might contribute to osmotic adjustments to act as osmoprotectants rather than osmolytes. These changes of metabolomics might function in many resistance and stress responses.

Keywords

Atriplex halimus Drought Growth Metabolomics NaCl Proline Sugars 

References

  1. Ahuja I, de Vos R, Bone AM, Hall RD (2010) Plant molecular stress responses face climate change. Trends Plant Sci 15:664–674PubMedCrossRefGoogle Scholar
  2. Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216CrossRefGoogle Scholar
  3. Bajji M, Kinet JM, Lutts S (1998) Salt stress effects on roots and leaves of Atriplexhalimus L. and their corresponding callus cultures. Plant Sci 137:131–142CrossRefGoogle Scholar
  4. Barbier-Brygoo H, Joyard J (2004) Focus on plant proteomics. Plant Physiol Biochem 12:913–917CrossRefGoogle Scholar
  5. Bates LE (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207CrossRefGoogle Scholar
  6. Ben Hassine A, Ghanem ME, Bouzid S, Lutts S (2009) An inland and a coastal population of the Mediterranean xero-halophyte species Atriplex halimus L. differ in their ability to accumulate proline and glycinebetaine in response to salinity and water stress. J Exp Bot 59:1315–1326CrossRefGoogle Scholar
  7. Ben Salah I, Slatni T, Gruber M, Messedi D, Gandour M, Benzarti M, Haouala R, Zribi K, Ben Hamed K, Perez-Alfocea F, Abdelly C (2011) Relationship between symbiotic nitrogen fixation, sucrose synthesis and anti-oxidant activities in source leaves of two Medicago ciliaris lines cultivated under salt stress. Environ Exp Bot 70:166–173CrossRefGoogle Scholar
  8. Bino RJ, Hall RD, Fiehn O, Kopka J, Saito K, Draper J, Nikolau BJ, Mendes P, Roessner-Tunali U, Beale MH, Trethewey RN, Lange BM, Wurtele ES, Sumner LW (2004) Potential of metabolomics as a functional genomics tool. Trends Plant Sci 9:418–425PubMedCrossRefGoogle Scholar
  9. Bradley PM, Morris JT (1991) Relative importance of ion exclusion, secretion and accumulation in Spartina alterniflora Loisel. J Exp Bot 42:1525–1532CrossRefGoogle Scholar
  10. Cramer G, Ergul A, Grimplet J, Tillett R, Tattersall E, Bohlman M, Vincent D, Sonderegger J, Evans J, Osborne C, Quilici D, Schlauch K, Schooley D, Cushman J (2007) Water and salinity stress in grapevines: early and late changes in transcript and metabolite profiles. Funct Integr Genomics 7:111–134PubMedCrossRefGoogle Scholar
  11. Dao TT, Puig RC, Kim HK, Erkelens C, Lefeber AW, Linthorst HJ, Choi YH, Verpoorte R (2009) Effect of benzothiadiazole on the metabolome of Arabidopsis thaliana. Plant Physiol Biochem 47:146–152CrossRefGoogle Scholar
  12. Debez A, Chaıbi W, Bouzid S (2003) Physiological responses and structural modifications in Atriplex halimus L. plants exposed to salinity. In: Lieth H, Moschenko M (eds) Cash crop halophytes: recent studies: 10 years after the Al Ain meeting. Kluwer, Dordrecht, pp 19–30Google Scholar
  13. Fiehn O, Weckwerth W (2003) Deciphering metabolic networks. Eur J Biochem 270:579–588PubMedCrossRefGoogle Scholar
  14. Gagneul D, Ainouche A, Duhaze C, Lugan R, Lahrer FR, Bouchereau A (2007) A reassessment of the function of the so-called compatible solutes in the halophytic Plumbginaceae Limonium latifolium. Plant Physiol 144:1598–1611PubMedCrossRefGoogle Scholar
  15. Gao XP, Pan QH, Li MJ, Zhang LY, Wang XF, Shen YY, Lu YF, Chen SW, Liang Z, Zhang DP (2004) Abscisic acid is involved in the water-stress-induced betaine accumulation in pear leaves. Plant Cell Physiol 45:742–750PubMedCrossRefGoogle Scholar
  16. Glenn EP, Brown JJ (1998) Effects of soil salt levels on the growth and water use efficiency of Atriplex canescens (Chenopodiaceae) varieties in drying soil. Am J Bot 85:10–16PubMedCrossRefGoogle Scholar
  17. Gong Q, Li P, Ma S, Rupassara SI, Bohnert H (2005) Salinity stress adaptation competence in the exptremophile Thellungiella halophila in comparison with its relative Arabidopsis thaliana. Plant Physiol 44:826–839Google Scholar
  18. Hall R, Beale M, Fiehn O, Hardy N, Sumner L, Bino R (2002) Plant metabolomics: the missing link in functional genomics strategies. Plant Cell 14:1437–1440PubMedCrossRefGoogle Scholar
  19. Hansen EH, Munns DN (1988) Effect of CaSO4 and NaCl on mineral content of Leucaena leucocephala. Plant Soil 7:101–105CrossRefGoogle Scholar
  20. Hare PD, Cress WA (1997) Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regul 21:79–102CrossRefGoogle Scholar
  21. Hare PD, Cress WA, Staden JV (1998) Dissecting the roles of osmolytes accumulation during stress. Plant, Cell Environ 21:535–553CrossRefGoogle Scholar
  22. Iyer S, Caplan A (1998) Products of proline catabolism can induce osmotically regulated genes in rice. Plant Physiol 116:203–211CrossRefGoogle Scholar
  23. Jasiniski M, Kachlicki P, Rodziewicz P, Figlerowicz M, Stobiecki M (2009) Changes in the profile of flavonoid accumulation in Medicago truncatula leaves during infection with fungal pathogen Phoma medicaginis. Plant Physiol Biochem 47:847–853CrossRefGoogle Scholar
  24. Kavi Kishore P, Sangam S, Amrutha R, Laxmi P, Naidu K, Rao K, Rao S, Reddy K, Theriappan P, Sreenivasulu N (2005) Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Curr Sci 88:424–438Google Scholar
  25. Kefu Z, Hai F, San Z, Jie S (2003) Study on the salt and drought tolerance of Suaeda salsa and Kalanchoe claigremontiana under iso-osmotic salt and water stress. Plant Sci 165:837–844CrossRefGoogle Scholar
  26. Khan M, Ungar I, Showalter A (2000) Effects of salinity on growth, water relations and ion accumulation of the subtropical perennial halophyte, Atriplex griffithii var. stocksii. Ann Bot 85:225–232CrossRefGoogle Scholar
  27. Kim JK, Bamba T, Harada K, Fukusaki E, Kobayashi A (2007) Time-course metabolic profiling in Arabidopsis thaliana cell cultures after salt stress treatment. J Exp Bot 58:415–424PubMedCrossRefGoogle Scholar
  28. Kozlowski TT, Pallardy SG (2002) Acclimation and adaptive responses of woody plants to environmental stresses. Bot Rev 68:270–334CrossRefGoogle Scholar
  29. Larher FR, Lugan R, Gagneul D, Guyot S, Monnier C, Lespinasse Y, Bouchereau A (2009) A reassessment of the prevalent organic solutes constitutively accumulated and potentially involved in osmotic adjustment in pear leaves. Environ Exp Bot 66:230–241CrossRefGoogle Scholar
  30. Louis P, Galinski EA (1997) Characterization of genes for the biosynthesis of compatible solute ectoine from Marinococcus halophilus and osmoregulated expression in E.coli. Microbiol 143:1141–1149CrossRefGoogle Scholar
  31. Low NH, McLaughlin M, Hofsommer H, Hammond DA (1999) Capillary gas chromatographic detection of invert sugar in heated, adulterated, and adulterated and heated apple juice concentrates employing the equilibrium method. J Agric Food Chem 47:4261–4266PubMedCrossRefGoogle Scholar
  32. Martinez JP, Ledent JF, Bajji M, Kinet JM, Lutts S (2003) Effect of water stress on growth, Na and K accumulation and water use efficiency in relation to osmotic adjustment in two populations of Atriplex halimus L. Plant Growth Regul 41:63–73CrossRefGoogle Scholar
  33. Martinez JP, Kinet JM, Bajji M, Lutts S (2005) NaCl alleviates polyethylene glycol-induced water stress in the halophyte species Atriplex halimus L. J Exp Bot 56:2421–2431PubMedCrossRefGoogle Scholar
  34. Munns R (2002) Comparative physiology of salt andwater stress. Plant, Cell Environ 25:239–250CrossRefGoogle Scholar
  35. Munns R (2005) Genes and salt tolerance: bringing them together. New Phytol 167:645–663PubMedCrossRefGoogle Scholar
  36. Oliver D, Nikolau B, Wurtele ES (2002) Functional genomics: high throughput mRNA, protein, and metabolite analyses. Metab Eng 4:98–108PubMedCrossRefGoogle Scholar
  37. Overy SA, Walker HJ, Malone S, Howard TP, Baxter CJ, Sweetlove LJ, Hill SA, Quick WP (2005) Application of metabolite profiling to the identification of traits in a population of tomato introgression lines. J Exp Bot 56:287–296PubMedCrossRefGoogle Scholar
  38. Rhodes D, Nadolska-Orczyk A, Rich PJ (2002) Salinity, osmolytes and compatible solutes. In: Laüchli A, Lüttge U (eds) Salinity: environment–plant–molecules. Kluwer, The Netherlands, pp 181–204Google Scholar
  39. Sanchez DH, Siahpoosh MR, Roessner U, Udvardi M, Kopk J (2008) Plant metabolomics reveals conserved and divergent metabolic responses to salinity. Physiol Plant 132:209–219PubMedGoogle Scholar
  40. Schauer N, Fernie AR (2006) Plant metabolomics: towards biological function and mechanism. Trends Plant Sci 11:508–516PubMedCrossRefGoogle Scholar
  41. Silveira JA, Araujo SA, Lima GP, Viegas RA (2009) Roots and leaves display contrasting osmotic adjustment mechanisms in response to NaCl-salinity in Atriplex nummularia. Environ Exp Bot 66:1–8CrossRefGoogle Scholar
  42. Stitt M (1990) Fructose 2,6-bisphosphate. In: Lea PJ (ed) Methods in plant biochemistry. Academic Press, London, pp 87–92Google Scholar
  43. Sweetlove LJ, Last RL, Fernie AR (2003) Predictive metabolic engineering: a goal for systems biology. Plant Physiol 132:420–425PubMedCrossRefGoogle Scholar
  44. Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6:66–71PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Mamdouh M. Nemat Alla
    • 1
  • Abdel-Hamid A. Khedr
    • 1
  • Mamdouh M. Serag
    • 1
  • Amina Z. Abu-Alnaga
    • 1
  • Reham M. Nada
    • 1
  1. 1.Botany DepartmentFaculty of Science, Mansoura UniversityDamiettaEgypt

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